ID A0A0D3CWI9_BRAOL Unreviewed; 1044 AA.
AC A0A0D3CWI9;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=glycine--tRNA ligase {ECO:0000256|ARBA:ARBA00012829};
DE EC=6.1.1.14 {ECO:0000256|ARBA:ARBA00012829};
OS Brassica oleracea var. oleracea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo6g086520.1, ECO:0000313|Proteomes:UP000032141};
RN [1] {ECO:0000313|EnsemblPlants:Bo6g086520.1, ECO:0000313|Proteomes:UP000032141}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F., Belcram H.,
RA Links M.G., Just J., Clarke C., Bender T., Huebert T., Mason A.S.,
RA Pires J.C., Barker G., Moore J., Walley P.G., Manoli S., Batley J.,
RA Edwards D., Nelson M.N., Wang X., Paterson A.H., King G., Bancroft I.,
RA Chalhoub B., Sharpe A.G.;
RT "Transcriptome and methylome profiling reveals relics of genome dominance
RT in the mesopolyploid Brassica oleracea.";
RL Genome Biol. 15:R77-R77(2014).
RN [2] {ECO:0000313|EnsemblPlants:Bo6g086520.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC EC=6.1.1.14; Evidence={ECO:0000256|ARBA:ARBA00000201};
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00008226}.
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DR AlphaFoldDB; A0A0D3CWI9; -.
DR STRING; 109376.A0A0D3CWI9; -.
DR EnsemblPlants; Bo6g086520.1; Bo6g086520.1; Bo6g086520.
DR Gramene; Bo6g086520.1; Bo6g086520.1; Bo6g086520.
DR eggNOG; ENOG502QS5T; Eukaryota.
DR HOGENOM; CLU_007220_0_0_1; -.
DR OMA; LPIPKRM; -.
DR Proteomes; UP000032141; Chromosome C6.
DR GO; GO:0009507; C:chloroplast; IEA:EnsemblPlants.
DR GO; GO:0005739; C:mitochondrion; IEA:EnsemblPlants.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IEA:EnsemblPlants.
DR GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:InterPro.
DR GO; GO:0045995; P:regulation of embryonic development; IEA:EnsemblPlants.
DR CDD; cd00733; GlyRS_alpha_core; 1.
DR HAMAP; MF_00254; Gly_tRNA_synth_alpha; 1.
DR HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR InterPro; IPR002310; Gly-tRNA_ligase_asu.
DR NCBIfam; TIGR00388; glyQ; 1.
DR NCBIfam; TIGR00211; glyS; 1.
DR PANTHER; PTHR30075:SF2; GLYCINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2; 1.
DR PANTHER; PTHR30075; GLYCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF02091; tRNA-synt_2e; 1.
DR Pfam; PF02092; tRNA_synt_2f; 2.
DR PRINTS; PR01044; TRNASYNTHGA.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 2.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000032141}.
FT REGION 44..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 444..465
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1044 AA; 117304 MW; 4CFD8DED332BDB61 CRC64;
MAILSISFPL IISSFLRPHA SPRFLHLPRY LPRSPYRRFH RTAVSSNVHH HPSHRKPDDN
DSNSVSIPTF QQAIQRLQEY WASVGCAVMQ PSNTEVGAGT MNPSTFLRVL GPEPWNVAYV
EPSIRPDDSR YGENPNRLQR HTQFQVILKP DPGNSQDLFI NSLSALGIDV TQHDIRFVED
NWESPVLGAW GLGWEIWMDG MEITQFTYFQ QAGSLQLSPV SVEITYGLER ILMLLQEVDH
FKKILYADGI TYGELFLENE KEMSSYYLEH ASVNRLQKHF DSFDEEARSL LALGLPIPAY
DQLLKTSHAF NILDARGFVG VTERARYFGR MRSLARQCAQ LWLKTRESLG HPLGVASEHI
PPIHHGAALE NVAEKVPEDP RSFIIEIGTE EMPPPDVINA SEQLRVLVVQ LLENQRLRHG
VVKAFGTPRR LVVLVDAMSP KQLEEEVEVR GPPASKAFDD QGNPTKAADG FSRRYGVPLE
KLYRKVDGKT EYVHARVTQP ARLALEVLSE ELPGILAKIS FPKSMRWNSS HVMFSRPVRW
VMALHGDLVV PFCFAGNTSG NVSFGLRNTA SATLLERKKI ILDKSNALAK SVNGRVVVQQ
NLLNEVANLV EAPVPLIGKF KESFLELPDE LLTIVMQKHQ KYFSITDESG KLLPYFIAVA
NGAINEDVVR KGNEAVLRAR YEDAKFFYEV DTRKRFSEFR DQLKGILFHE KLGTMLDKMN
RLEKMVTKLC LALEVDEDLL PVVEEAASLA LSDLATSVVT EFTSLSGIMA RHYALRDGYS
EQIAEALLEI TLPRFSGDVI PKTDAGMVLA IGDRLDSLVG LFAAGCQPSS TNDPFGLRRI
SYGLVQILVE KDKNVNFKHA LDIAASVQPI KVEANTLDDV YQFVTRRLEQ LLVDNGVSPE
VVRSVLAERG NDPCLAARTA YKMEKLSKGE IFPKIVEAYS RPTRIVRGKD VDVGVEVDES
VFETSQEKEL WSIYTSIKDR IHSGIGIEEF TEISTQLVEP LEDFFNNVFV MVEEERVRKN
RLALLNNIAS LPSGIADLSF LPGF
//
