ID A0A0D3DFI9_BRAOL Unreviewed; 2185 AA.
AC A0A0D3DFI9;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=separase {ECO:0000256|ARBA:ARBA00012489};
DE EC=3.4.22.49 {ECO:0000256|ARBA:ARBA00012489};
OS Brassica oleracea var. oleracea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo7g108380.1, ECO:0000313|Proteomes:UP000032141};
RN [1] {ECO:0000313|EnsemblPlants:Bo7g108380.1, ECO:0000313|Proteomes:UP000032141}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F., Belcram H.,
RA Links M.G., Just J., Clarke C., Bender T., Huebert T., Mason A.S.,
RA Pires J.C., Barker G., Moore J., Walley P.G., Manoli S., Batley J.,
RA Edwards D., Nelson M.N., Wang X., Paterson A.H., King G., Bancroft I.,
RA Chalhoub B., Sharpe A.G.;
RT "Transcriptome and methylome profiling reveals relics of genome dominance
RT in the mesopolyploid Brassica oleracea.";
RL Genome Biol. 15:R77-R77(2014).
RN [2] {ECO:0000313|EnsemblPlants:Bo7g108380.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=All bonds known to be hydrolyzed by this endopeptidase have
CC arginine in P1 and an acidic residue in P4. P6 is often occupied by
CC an acidic residue or by a hydroxy-amino-acid residue, the
CC phosphorylation of which enhances cleavage.; EC=3.4.22.49;
CC Evidence={ECO:0000256|ARBA:ARBA00000451};
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DR STRING; 109376.A0A0D3DFI9; -.
DR EnsemblPlants; Bo7g108380.1; Bo7g108380.1; Bo7g108380.
DR Gramene; Bo7g108380.1; Bo7g108380.1; Bo7g108380.
DR eggNOG; KOG1849; Eukaryota.
DR HOGENOM; CLU_001952_0_0_1; -.
DR OMA; SKWIETE; -.
DR Proteomes; UP000032141; Chromosome C7.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0098813; P:nuclear chromosome segregation; IEA:UniProt.
DR GO; GO:0000280; P:nuclear division; IEA:UniProt.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR InterPro; IPR005314; Peptidase_C50.
DR InterPro; IPR030397; SEPARIN_core_dom.
DR PANTHER; PTHR12792; EXTRA SPINDLE POLES 1-RELATED; 1.
DR PANTHER; PTHR12792:SF0; SEPARIN; 1.
DR Pfam; PF03568; Peptidase_C50; 1.
DR PROSITE; PS51700; SEPARIN; 1.
PE 4: Predicted;
KW Chromosome partition {ECO:0000256|ARBA:ARBA00022829};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000032141}.
FT DOMAIN 1926..2029
FT /note="Peptidase C50"
FT /evidence="ECO:0000259|PROSITE:PS51700"
FT REGION 2104..2131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2185 AA; 245266 MW; EBB35AE520FE4577 CRC64;
MSSDDHHRLL SLIESSDDIF TSFSDYLRPF TSPQPSSRSL GKQFLPFLNK SISLFPKRLS
ATANPQARES AGDLFRAYEL CLDCLESFSA QLACKPHTLQ LQRLRMIYCL DAWGFHESVI
VQAFKVVEKL RGGEVGSKLL PEVKEGEAEL AMVLVEAVAA VFKGVAMCKQ VDDEPYRTVL
LMVEEVRDWF RVLDAKAYEK LHRVLVTNLG KCALSLVREA ARFDGGLVRS FCDATVKEHY
KFALSKDRIY KFARDVLSVL FGFKDRRMSM TIDISMSVLR SLTCNFEVKT NEDIVDFVEH
VSYCAHKFRS AGDVYCIQVS KRLNEMAAIF SEAIPQLNLI LRLYSSGLSI MVYDSRESKV
KDATDDWKIQ ALLDDETRWQ NLVSLLGMVD QTDLGNQTDL SLVGGHKSYL SKTNDSCSGI
NMKNWWLQYV DALKFLCLPL ATLITSVKRK IVLGTEMSCA SAHLSTIHDA FLQFCDGCLF
LQRCTSEKGG SETDNNKALL NVAMAAFIVS LRTQQKLEIS VHLVENVIAS PWIGSQELKY
ILASLYNVGV VLYRNKELKK ACEALKLCSK ASWRCVELDC QIFVNQSSSP ELSEDAIMDF
VGETCNRSAF YLDVLQQCSR CKIRQTIVHI LENWLSAEHL MRRLPGPAAI VKQWVKIERG
CHTNLDMVDS CTTLYSLLSS SKKKSNRAIG KILQQELLAY DEMFSLSSKL GQQTRIEIAD
ILLKNVYVTE DMHVERARIL VWKARITRAS GTEHLADCIR FLSEAISILS EVHHGPNKEG
VSSSYQLPIA YCLRAFCTQE AEPNSKKVFQ DISTSLNLWL RIPSLDDSED SLPTENIVPL
LYNMFDLMSV KGCTELHHHI YQLIFRLFKR KHVKLEVCLG MMWESRRLSH ALCPSPISDA
FILGLSENWG DKSTCIDFWI DCLKDSKARL IGFQQNFHVL QNDFLRSSKK DKGHFHSDIT
IDDITDAASE LISSASLSGQ SSFTAAYLYY DLSERLISFG KLSEALSYAK EAYRIRTLLF
QEKFKYTAEK QFEKYNDAGK ISEIRSYSIT DFQVYRSLAT NFWPCGNFSW DINHCYLSRW
NVLQCYLEST LQVGIANELI GNGLEAESLL SWGKAISCSQ SLFPFVVAFS SALGIFYHKK
QSLDLAEKEI QNAKEILVTN QREFSCIKCK LRVEVTLDKQ LGDISRTQID RVSQTDGFLH
AESLFSAALG KICCSAWKSC IRSHGEDITD GTAIDKNGGE VLEHKARKTK LCVNKEPTES
KGSRKGRRAK ASQTCVSKEH YLISEPTSRL TRSMRQSRKD QCQNCSMPEV VSKKPSFCDL
SDYSGAESVL LDTKNTVHGF CICYKGKCMQ CLSVKVLASG SLNSLISLKW ELCHRRLASS
ILVNLGKCLA DSGRVHLAHE ALLHSISVLF KSNRSSHNQP SVSELLEFIG QEATMDVFAI
DRAIILYNLC WFSLRNYHCR ESRFICCDLS QIPFPKLVSW LTLAFILSRE VPTVFQKVSR
LLASLYMLSS SSDEFSFLCD GKELSTSHWV SYFHQASLGT HISYQFISNL SRGHKPQCLS
DKECTEATCS SCMVPEELDL PRLAPERTQD LVQFAKEFFS NLPSSTIICI SLLGGALNEL
LQELMQIRSP VCAWVLVSRL TLKSQPVATL LPVDSVLEDM SDDDSATISS TEATQVKNLE
RRWLCPWGST VVDDVAPAFK SILEESHISS GSPVEDTREH RNSWWKKRKT LDHHLKKFLR
NLEASWLGPW RCLLLGDLSN FKLPDSVQKK LVKDLKSKCK MEVNEMLLKV LLGGGIENFE
GEACVAQLSL SNGCYVGRGG YLYEEDSCKT PTAASTSESR HGLALQLIRE AATKLEQHDG
CDNREPIILV LDPEVQMLPW ENIPILRKQE VYRMPSVGSI FSVLKKRCLQ GEPAKSQAAS
FPLIDPLDSF YLLNPGGDLS ETQVEFESWF RDQNFEGKAG SVPSAEELTE ALKSHDLFLY
FGHGSDLLLY LILAGSQYLS SREIEKLENC CATFLMGCSS GSLWLKGCYI PQGMPLSYLL
AGSPAIVANL WDVTDRDIDR FGKALLEAWL RERSDSPSPF SSEGGCSQCE SLTNELAAMN
LKGNTTKRTR KPSSRNKPAQ SNADGSGKME CNHNHGRKIG SFIAAAREVC TLPYLIGAAP
VCYGVPTGIT RKKGIEALLP SSSSC
//
