ID   A0A0D6DXQ0_9LACT        Unreviewed;       757 AA.
AC   A0A0D6DXQ0;
DT   27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT   27-MAY-2015, sequence version 1.
DT   27-MAR-2024, entry version 42.
DE   RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU364053};
DE            EC=5.6.2.4 {ECO:0000256|RuleBase:RU364053};
GN   Name=pcrA {ECO:0000313|EMBL:CEN28543.1};
GN   ORFNames=LACPI_1343 {ECO:0000313|EMBL:CEN28543.1};
OS   Lactococcus piscium MKFS47.
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=297352 {ECO:0000313|EMBL:CEN28543.1, ECO:0000313|Proteomes:UP000033166};
RN   [1] {ECO:0000313|Proteomes:UP000033166}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKFS47 {ECO:0000313|Proteomes:UP000033166};
RA   Andreevskaya M.;
RL   Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00034618,
CC         ECO:0000256|RuleBase:RU364053};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC         translocating in the 3'-5' direction.; EC=5.6.2.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00034617};
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000256|ARBA:ARBA00009922, ECO:0000256|RuleBase:RU364053}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; LN774769; CEN28543.1; -; Genomic_DNA.
DR   RefSeq; WP_047915660.1; NZ_LN774769.1.
DR   AlphaFoldDB; A0A0D6DXQ0; -.
DR   STRING; 1364.LP2241_30355; -.
DR   KEGG; lpk:LACPI_1343; -.
DR   HOGENOM; CLU_004585_5_2_9; -.
DR   Proteomes; UP000033166; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR   CDD; cd17932; DEXQc_UvrD; 1.
DR   CDD; cd18807; SF1_C_UvrD; 1.
DR   Gene3D; 1.10.10.160; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   NCBIfam; TIGR01073; pcrA; 1.
DR   PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR   PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR   Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW   ProRule:PRU00560}; DNA-binding {ECO:0000256|RuleBase:RU364053};
KW   Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU00560}; Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW   ProRule:PRU00560}.
FT   DOMAIN          6..284
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51198"
FT   DOMAIN          285..568
FT                   /note="UvrD-like helicase C-terminal"
FT                   /evidence="ECO:0000259|PROSITE:PS51217"
FT   BINDING         27..34
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ   SEQUENCE   757 AA;  85356 MW;  06D8A5523BB20302 CRC64;
     MNPLLQGMNA RQAEAVEATE GPLLIMAGAG SGKTRVLTHR IAYMIEEKEV NPWNILAITF
     TNKAAREMRE RAINLNPRAQ DSLIATFHSM CVRILRRDAD HIGYNRNFTI VDPGEQRTLM
     KRILKELNVD PKQFTERTIL GTISNSKNEL VTAEIYEKNA DDLYTNIVAK AYKRYETELK
     RSESMDFDDL IMNTILLFDK NPDVLAYYQG KFQYIHVDEY QDTNHAQYQM VQLLAKRFKN
     LCVVGDADQS IYGWRGADMQ NILDFEKDYP QAKVVLLEEN YRSTKTILQA ANEVINHNKN
     RRPKELWTQN ADGEMIVYNR SGDEREEANF VAGQINYEQD QHNRSYADFA VLYRTNAQSR
     AIEEALLKSN IPYTMVGGTK FYSRKEIRDV VAYLNVIANS RDNISFERIV NEPKRGVGPK
     ALETLRDFAT QQNSSLLDAS RDVTLSQLKG KAAAELFNLA HVLLNLADDV NQFTITELVE
     AVLDKTGYRQ ALALQTTSLE AQNRLENIEE FLTVTQNFDK KEAEAPAEDE TGLDKLSRFL
     NDLSLVADTD SYEETAQVTL MTLHAAKGLE FPVVFLVGME EGVFPLSRQA EDADELEEER
     RLAYVGITRA EAVLYLTNAS TRMLFGRTSY NQPSRFLREI DDSLLTYTGQ ARKANTSFNA
     TYKSSVSQSQ FGSGVSLSDA IKGRKSATSS VKQALGQKPK AVADWGIGDI AQHKKWGDGT
     VLDVSGTGSN MELKIAFPEV GLKRLLAQMA PITKVEK
//