ID A0A0D8HHN9_9ACTN Unreviewed; 595 AA.
AC A0A0D8HHN9;
DT 27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT 27-MAY-2015, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE SubName: Full=Acetolactate synthase isozyme 3 large subunit {ECO:0000313|EMBL:KJF17428.1};
DE EC=2.2.1.6 {ECO:0000313|EMBL:KJF17428.1};
GN Name=ilvI {ECO:0000313|EMBL:KJF17428.1};
GN ORFNames=AXFE_17060 {ECO:0000313|EMBL:KJF17428.1};
OS Acidithrix ferrooxidans.
OC Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales;
OC Acidimicrobiaceae; Acidithrix.
OX NCBI_TaxID=1280514 {ECO:0000313|EMBL:KJF17428.1, ECO:0000313|Proteomes:UP000032360};
RN [1] {ECO:0000313|EMBL:KJF17428.1, ECO:0000313|Proteomes:UP000032360}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Py-F3 {ECO:0000313|EMBL:KJF17428.1,
RC ECO:0000313|Proteomes:UP000032360};
RA Poehlein A., Eisen S., Schloemann M., Johnson B.D., Daniel R., Muehling M.;
RT "Draft genome of the acidophilic iron oxidizer Acidithrix ferrooxidans
RT strain Py-F3.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937;
CC Evidence={ECO:0000256|ARBA:ARBA00001964};
CC -!- SIMILARITY: Belongs to the TPP enzyme family.
CC {ECO:0000256|ARBA:ARBA00007812, ECO:0000256|RuleBase:RU362132}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KJF17428.1}.
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DR EMBL; JXYS01000042; KJF17428.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0D8HHN9; -.
DR STRING; 1280514.AXFE_17060; -.
DR PATRIC; fig|1280514.3.peg.2237; -.
DR Proteomes; UP000032360; Unassembled WGS sequence.
DR GO; GO:0003984; F:acetolactate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
DR CDD; cd00568; TPP_enzymes; 1.
DR CDD; cd07035; TPP_PYR_POX_like; 1.
DR Gene3D; 3.40.50.970; -; 2.
DR Gene3D; 3.40.50.1220; TPP-binding domain; 1.
DR InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR InterPro; IPR029061; THDP-binding.
DR InterPro; IPR012000; Thiamin_PyroP_enz_cen_dom.
DR InterPro; IPR012001; Thiamin_PyroP_enz_TPP-bd_dom.
DR InterPro; IPR000399; TPP-bd_CS.
DR InterPro; IPR045229; TPP_enz.
DR InterPro; IPR011766; TPP_enzyme_TPP-bd.
DR PANTHER; PTHR18968:SF13; ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL; 1.
DR PANTHER; PTHR18968; THIAMINE PYROPHOSPHATE ENZYMES; 1.
DR Pfam; PF02775; TPP_enzyme_C; 1.
DR Pfam; PF00205; TPP_enzyme_M; 1.
DR Pfam; PF02776; TPP_enzyme_N; 1.
DR SUPFAM; SSF52467; DHS-like NAD/FAD-binding domain; 1.
DR SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 2.
DR PROSITE; PS00187; TPP_ENZYMES; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000032360};
KW Thiamine pyrophosphate {ECO:0000256|RuleBase:RU362132};
KW Transferase {ECO:0000313|EMBL:KJF17428.1}.
FT DOMAIN 18..131
FT /note="Thiamine pyrophosphate enzyme N-terminal TPP-
FT binding"
FT /evidence="ECO:0000259|Pfam:PF02776"
FT DOMAIN 215..349
FT /note="Thiamine pyrophosphate enzyme central"
FT /evidence="ECO:0000259|Pfam:PF00205"
FT DOMAIN 417..570
FT /note="Thiamine pyrophosphate enzyme TPP-binding"
FT /evidence="ECO:0000259|Pfam:PF02775"
SQ SEQUENCE 595 AA; 64357 MW; 1C1705094CDE2C33 CRC64;
MTVESEKVAS IDVTNPSMTG AEALVKQLEL YGVEYVFGLC GHTNIAVLDA LSRSPIEFII
NRHEQTAAHA ADGYARASGK PGVLLSHVGP GMINAATGVY TAALDSVPLI AIAGDVPSYY
YGRHPHQEIN IHNNASQADV YRPFVKRAWN VERVEDLVRF TERAFWTATS GRPGAVLLNV
PMDMFSRRLP STYNFPPPLP ARSTSPRLGY GVAESIATML VDANRPLIYL GGGIRTKEGL
AALVSLAEHL DIPIAHSLMG KGAVSDDHPL VIGMTGFWGS TFTNGYAREA DVVLGLATRF
AETDSSSWMP EYTWQFPPSR LIQIDIDPDE IGRNYPVEIG AVADVNQAVI EIEAALKIMA
KDGVNKPELR EDIKAMREGL WARTKENGSS DQFPLRPERI LQDLLETLPS DAVLVTDVGW
NKNGVAQGYK LPSQGKFITP GGASTMGFGP AGAIGVQIAL PDRTVIALIG DGGMSAQLPA
VPTAVEQGLP IIWVVMNNRA HGTIADLEYG NYGHSFGTIF QDRNGASYSP DFAAYARACG
ADGYAISSPQ EFKKSLLDAL ELRRPALLDV PMVNEPVPTP GHWNINDIYQ GRFFD
//
