ID A0A0D9QKE9_PLAFR Unreviewed; 1614 AA.
AC A0A0D9QKE9;
DT 27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT 27-MAY-2015, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE RecName: Full=SAM-dependent methyltransferase TRM5/TYW2-type domain-containing protein {ECO:0000259|PROSITE:PS51684};
GN ORFNames=AK88_04157 {ECO:0000313|EMBL:KJP86186.1};
OS Plasmodium fragile.
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Plasmodium).
OX NCBI_TaxID=5857 {ECO:0000313|EMBL:KJP86186.1, ECO:0000313|Proteomes:UP000054561};
RN [1] {ECO:0000313|EMBL:KJP86186.1, ECO:0000313|Proteomes:UP000054561}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=nilgiri {ECO:0000313|Proteomes:UP000054561};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Neafsey D., Duraisingh M., Young S.K., Zeng Q., Gargeya S., Abouelleil A.,
RA Alvarado L., Chapman S.B., Gainer-Dewar J., Goldberg J., Griggs A.,
RA Gujja S., Hansen M., Howarth C., Imamovic A., Larimer J., Pearson M.,
RA Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The Genome Sequence of Plasmodium fragile nilgiri.";
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in
CC tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-
CC carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:36635, Rhea:RHEA-COMP:10378, Rhea:RHEA-
CC COMP:10379, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73543, ChEBI:CHEBI:73550; EC=2.1.1.282;
CC Evidence={ECO:0000256|ARBA:ARBA00001168};
CC -!- PATHWAY: tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004797}.
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DR EMBL; KQ001699; KJP86186.1; -; Genomic_DNA.
DR RefSeq; XP_012337190.1; XM_012481767.1.
DR EnsemblProtists; KJP86186; KJP86186; AK88_04157.
DR GeneID; 24269471; -.
DR VEuPathDB; PlasmoDB:AK88_04157; -.
DR OMA; KCMFCSG; -.
DR OrthoDB; 122704at2759; -.
DR UniPathway; UPA00375; -.
DR Proteomes; UP000054561; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0102522; F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 2.120.10.80; Kelch-type beta propeller; 2.
DR Gene3D; 3.30.1960.10; tRNA wybutosine-synthesizing-like; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR011043; Gal_Oxase/kelch_b-propeller.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR030382; MeTrfase_TRM5/TYW2.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR003827; tRNA_yW-synthesising.
DR InterPro; IPR036602; tRNA_yW-synthesising-like_sf.
DR PANTHER; PTHR23245; TRNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR23245:SF31; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3 HOMOLOG; 1.
DR Pfam; PF13418; Kelch_4; 1.
DR Pfam; PF02475; Met_10; 1.
DR Pfam; PF02676; TYW3; 1.
DR SUPFAM; SSF50965; Galactose oxidase, central domain; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR SUPFAM; SSF111278; SSo0622-like; 1.
DR PROSITE; PS51684; SAM_MT_TRM5_TYW2; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 1248..1606
FT /note="SAM-dependent methyltransferase TRM5/TYW2-type"
FT /evidence="ECO:0000259|PROSITE:PS51684"
FT REGION 427..474
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 926..949
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1516..1541
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1614 AA; 184337 MW; 2D4C5306F1EF646F CRC64;
MEAFLRKKKK AEKVVDMERE LHRYTCIYEN CKEDVCNIYL NIYRKCKEGV EEKAEEVLEE
NPRGMEEMVK RVVEQKNIND DGEDKSIKKS IDLLITPCIY EINRSPDYFT SSCCSGRVVI
FGEVGGVREN RNSCSGAMCN RSGSVVGGAD LGDNANGKTK RTIEQDAFIS YLKRSYHEEG
CHSYDECDPP VQGRNISPER TKKKHRKNVH MYYSSHMHQN LREDPKVVKR ILTQCLNGVT
TSPLVSYEKE EDSVVVNTKW GSPSEARKEL PRGHHSREEL IYTNDEDVTK MSRKKKKTRI
FFKFEPFIIH VKCVNLVSAL RLLKVAQMAG LKQSGLLNFN RDVTVAIRGS MRLEHYVDDA
LSLEENSIAK LLDVCNGKMN HNLRQLVGFY HCYRESMVGG GSKWQPLHLS QLQCEKCAQM
CGCSQREEHP PQQSIKNDLA GSGESEEKNG SPITLPDTQW EKNRSTKEKK KKKKEKEKGE
IYLNKYDIEL SDEGHIRTGT CPMGADSSLE WKLLSSEGDL DKLFVWGHDM FIEEGKIYLF
GGFAKGVRSR QLKVFDVGRK HLRTYETPLP ALSYHSFFRL DSNYACVFGG RRSPESCTSD
VWLYDMRRNA WCDVKWRSSG EKRHGDGCGN SEDSSQPCGR YRHACALVRR YRKKQKEVYV
FYVHGGVTEK NEVLNDLWEG KLTVQVDTSM GTTAFIEWER KHSSEKATEG STEAPSPCLK
NHTMVYNKRR NLIYIVGGCS SSRAAGAHSS QACNEDRMEY LYTYDVKRDC FFYVPCGGSG
EDGPAALPLN RFSHATCAWG HNDFVLMGGL NMHRTLNDVW LFRMKQNKWY RLGVFPFHSM
YVRAKVASEG DYLYVVGGGC TVFTFGSFFD LPVVANCRRA LLEQGVGMGV DQGVSEWKHK
EVDNPADNSA DQEAGEAPDL ATSYFSLQQR RQRPRRTSPR SASQIRMDTH AGKKTQQDVL
YLIAKDRIYV KEVKTALERI GVFDKRRKIE VLKGRTSGEK DEDSFLVPVL EKIETRKNYE
QVRRVFPFEK VQLTKEGHIF YIHRSSKGVE EKTSLKQCLT NLFYNFANGM VKKYLSESER
KLIVRASAKY EVVGGIVIFH YKHVGPILHL YRTCVEGIAL KYTDRGTGKY SRLRTLLKKC
RRMKVISYVD ALWLDVRDAF NRFRFRGGHT TGAARRKERL RMRCNGTFLK FSSLARRRTV
LRSVAASSLW SSASPCRGTE QCSCGGREKC HSWGREKVCL LNHPWCSLTY VGRRAVKVEL
VAQIKKQLKD VRESKVGGKN NPAGNRIKAI TIYERIEGEK RKNRIHLVLG RNAKTIHVEN
NVVYKLDLQK CMFCSGNGTE KERMQNLYLH RANMVVSQEN VADLFCGVGY FTLPLLKFVG
EGKIKEYFAC DINGDSLRLL KSAVGLNKIK KTHLHILRQN SFVVTKNAQL VRRCHRVLLG
LLPHSVEAWC NAFHLLDGTV GGTLHIHGVG ENVFEEVFVS RVYTYDYVQS VKRVSETDVR
NMSLTELVQH GLHVTDGPQS ATTSIEQSCP RGGAKLGKKA KDPYRGNNVP ASLHFAQFVL
LEFFKLALMD YLAHKKHWVV SILHVERVKS YAPRLFHYVV DVRCVPEERV GEEV
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