ID   A0A0E0NJ00_ORYRU        Unreviewed;       595 AA.
AC   A0A0E0NJ00;
DT   27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT   27-MAY-2015, sequence version 1.
DT   27-MAR-2024, entry version 35.
DE   RecName: Full=Pseudouridine synthase I TruA alpha/beta domain-containing protein {ECO:0000259|Pfam:PF01416};
OS   Oryza rufipogon (Brownbeard rice) (Asian wild rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX   NCBI_TaxID=4529 {ECO:0000313|EnsemblPlants:ORUFI02G28710.1, ECO:0000313|Proteomes:UP000008022};
RN   [1] {ECO:0000313|Proteomes:UP000008022}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. W1943 {ECO:0000313|Proteomes:UP000008022};
RA   Zhao Q.;
RL   Submitted (JUN-2013) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EnsemblPlants:ORUFI02G28710.1}
RP   IDENTIFICATION.
RG   EnsemblPlants;
RL   Submitted (JUN-2015) to UniProtKB.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC       {ECO:0000256|ARBA:ARBA00009375}.
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DR   AlphaFoldDB; A0A0E0NJ00; -.
DR   STRING; 4529.A0A0E0NJ00; -.
DR   EnsemblPlants; ORUFI02G28710.1; ORUFI02G28710.1; ORUFI02G28710.
DR   Gramene; ORUFI02G28710.1; ORUFI02G28710.1; ORUFI02G28710.
DR   eggNOG; KOG2553; Eukaryota.
DR   HOGENOM; CLU_021971_2_0_1; -.
DR   OMA; NKAFDCR; -.
DR   Proteomes; UP000008022; Unassembled WGS sequence.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IEA:UniProt.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:InterPro.
DR   CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR   Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR   Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   InterPro; IPR041708; PUS1/PUS2-like.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR   PANTHER; PTHR11142:SF4; PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 1.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE   3: Inferred from homology;
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008022};
KW   tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT   DOMAIN          385..490
FT                   /note="Pseudouridine synthase I TruA alpha/beta"
FT                   /evidence="ECO:0000259|Pfam:PF01416"
FT   REGION          1..45
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          208..260
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        208..236
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        108
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR641708-1"
FT   BINDING         164
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR641708-2"
SQ   SEQUENCE   595 AA;  65413 MW;  F6E1BA0FA71B9DAC CRC64;
     MAAASPPASP PHPKRRKMSS SSDPDQEPTS PSAAGADGAQ PRAPRYKRRK VAILLGYCGA
     GYQGMQKNPG ARTIEGDLEE ALYRAGAVPE ADRAAPRRYD WARAARTDKG VSAAAQVVSG
     RFYVDPPVFT DRLNAQLAPQ IRAFGYVRVT NSFNAKKFCD RRRYVYLLPV FALDPSAHPD
     REAVMASVGS GSELTKCLEC SERGRKVPGV MGREGKVPDP ALQEEKSVEA SREEAAVGSN
     EDANCDPAST GDRSVDLPIP DNETNTQITE MRSNGAEKCD VQLSGGTLCS EAVPASNSET
     VCPILNSDSN TNSDSVDVDP SFVAEKKGSI ETVVTEDGKK QAMDVVTEDE KKQDVDYEGS
     SLEEMPLRSA FSYTHEVKER FNRILKKYVG THNFHNFTTR TKAEDPAAKR FIISFAADRV
     VNLDGIDFIR CEVVGQSFML HQIRKMIGLA VAVMRNCAPE SIYDVAFRKD VNLNVPTAPE
     VGLYLDECMF TSYNKKWKDS HEAVSMEPYS EEAEEFKLKY IFPHIAAMEH KEGAVALWLH
     SLNSRNYPDF RYMDNAGTEA SIAAAVESVD EGTAVSWQVM TPEKCFCNVC LLKLS
//