ID A0A0E0QEI3_ORYRU Unreviewed; 1214 AA.
AC A0A0E0QEI3;
DT 27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT 27-MAY-2015, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
OS Oryza rufipogon (Brownbeard rice) (Asian wild rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX NCBI_TaxID=4529 {ECO:0000313|EnsemblPlants:ORUFI08G03710.5, ECO:0000313|Proteomes:UP000008022};
RN [1] {ECO:0000313|Proteomes:UP000008022}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. W1943 {ECO:0000313|Proteomes:UP000008022};
RA Zhao Q.;
RL Submitted (JUN-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblPlants:ORUFI08G03710.5}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (JUN-2015) to UniProtKB.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460, ECO:0000256|RuleBase:RU004279}.
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DR AlphaFoldDB; A0A0E0QEI3; -.
DR EnsemblPlants; ORUFI08G03710.5; ORUFI08G03710.5; ORUFI08G03710.
DR Gramene; ORUFI08G03710.5; ORUFI08G03710.5; ORUFI08G03710.
DR Proteomes; UP000008022; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IEA:InterPro.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.1360.140; -; 1.
DR Gene3D; 4.10.320.40; -; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 2.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR000684; RNA_pol_II_repeat_euk.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007073; RNA_pol_Rpb1_7.
DR InterPro; IPR038593; RNA_pol_Rpb1_7_sf.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF37; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 3.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04990; RNA_pol_Rpb1_7; 1.
DR Pfam; PF05001; RNA_pol_Rpb1_R; 7.
DR PRINTS; PR01217; PRICHEXTENSN.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 2.
DR PROSITE; PS00115; RNA_POL_II_REPEAT; 8.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000008022};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 249..459
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 164..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 949..1043
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1164..1214
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..30
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 960..1043
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1164..1204
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1214 AA; 133724 MW; 9B3B8A19011B073E CRC64;
MAPFPAAADV GGKDDDDSGG GGEQHDGAAD DAGVDYAPPK THPPWWAAAA GEMDARFPYS
PAKVAKVQLV QFGILSPDEI RQMSVVQIEH AETMERGKPK PGGLSDPRLG TIDQKIKCET
CMAGMAECPG HFGHLELAKP MFHIGFIKTS KKVCAGGDDL DVQEQQDTDE PVKKRGGCGA
QQPNITVDGM KMVAEFKAPK KKNDDQEQLP EPVERKQILS AERVLNVLKR ISDEDCLLLG
LNPKFARPDW MILQVLPIPP PPVRPSVMMD TSSRSEDDLT HQLAMIIRHN ENLRRQERNG
APAHIITELK ELVEYGPHPP PGKTGAKYII REDGQRLDLR YVKKSSDQHL ELGYKVERHL
NDGDFVLFNR QPSLHKMSIM GHRIKIMPYS TFRLNLSVTS PYNADFDGDE MNMHVPQSFE
TRAEVLELMM VPKCIVSPQS NRPVMGIVQD TLLGCRKITK RDTLIEKDVF MNILMWWEDF
DGKVPAPAIL KPRPIWTGKQ VFNLIIPKPI NLIRFSAWHS EAETGFITPG DTMVRIEKGE
LLSDDVKELI KQAYDRQLEA EPGRTMMESF ENRVNQVLNK ARDDAGSSAQ KSLSESNNLK
AMVTAGSKGS FINISQMTAC VGQQNVEGKR IPFAFIDRTL PHFTKDDYGP ESRGLREIIN
VAKKIKTPSL SVYLKPEVNK KKELAKNVQC ALEYTTLRSV THATEIWYDP DPLGTIIEED
VEFWGEMDAR FPYSPAEVAK VQLVQFGILS PDEIRQMSVV QIEHAETMER GKPKPGGLSD
PRLGTIDRKI KCETCMAGMA ECPGHFGHLE LAKPMFHIGF IKTVLSIMRC VCFNCSKILV
DELAPIGTGG CALYLNDQML QQAIELQLPS YVEGLDFGMT PARSPISGTP YHEGMMSPSY
LLSPNIRASP ITDAQFSPYV GGMAFSPVPS PGYTPSSGGG YSPSSPVYSP GPGYSPTSPS
YSPASPSYSP TSPSYTPGSP TYSPTSPSYS PTSPSYSPTS PSYSPTSPSY SPTSPSYSPT
SPSYSPTSPS YSPTSPSYSP TSPPHITILQ PHITIIQSNV SVLQPHFPLI QSDITLIQSD
ISCIQPYITW LQPDISKLQP NFTKLQSNFT KLQSFFGQVQ SFPRVLSKQP KAEPLQSDFT
KLQPNIADIL TYLTIIFTTN PYTTSGGPSP DYSPTSPNYS PSGSYSPTAP GYSPSSTGQQ
FSPQAADKDK EKAK
//
