ID A0A0E3W3T6_9FIRM Unreviewed; 497 AA.
AC A0A0E3W3T6;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE SubName: Full=Radical SAM, alpha/beta horseshoe {ECO:0000313|EMBL:CFY03979.1};
GN ORFNames=2631 {ECO:0000313|EMBL:CFY03979.1};
OS Syntrophomonas zehnderi OL-4.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Syntrophomonadaceae;
OC Syntrophomonas.
OX NCBI_TaxID=690567 {ECO:0000313|EMBL:CFY03979.1, ECO:0000313|Proteomes:UP000045545};
RN [1] {ECO:0000313|EMBL:CFY03979.1, ECO:0000313|Proteomes:UP000045545}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OL-4 {ECO:0000313|EMBL:CFY03979.1,
RC ECO:0000313|Proteomes:UP000045545};
RA Murphy D.;
RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CGIH01000047; CFY03979.1; -; Genomic_DNA.
DR RefSeq; WP_046499753.1; NZ_CGIH01000047.1.
DR AlphaFoldDB; A0A0E3W3T6; -.
DR OrthoDB; 9801424at2; -.
DR Proteomes; UP000045545; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409:SF7; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000045545}.
FT DOMAIN 1..140
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 192..417
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 497 AA; 57061 MW; 1BBA564EDF88877F CRC64;
MKDTVMLIAF YNQKALGVRY LAEALKKNGY KVVAVFFKDF NSISPSKVSE KELQLLVELI
NKTEPSFIGL SVMSSLYLES VYLVNDTIKK YFNLPVVWGG VYATLFPDHA LEYADYVIRG
EGEEAIGELF DTLREGEDPS QMANLAYKDS SGQIVVNDVR PIVDVDELGF PSIGDLDFYS
INADKIAVGD PQMGGFTYEL TASRGCPYKC SYCSSINLRR IYKGKGKYMR FRSVDSVLAE
LNQAKKALPN LKVIHFWDEI FSNEPGWVEE FSQRYAQEIG IPFRVWGHPL MVNHRIISNL
VEAGLYQIVV GIQSGSPRIR KEVFQRNESQ EEIINCSRVL AECKVPKVIY DFMIHHPFES
LEELKETYYL CLELEPPFEL QIHGLHFLPG TDIVQMALDA GYYTYEEMNA KMYGSLEEQF
NSWYWDVSSS DNIDELTCWT SMIFLTQFPA LRPILKKLES NLQNNRGLKN IYRLHLIMNR
MLRLRNLGQK AKLVLKP
//
