ID A0A0E3WTM3_9EURY Unreviewed; 1017 AA.
AC A0A0E3WTM3;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=protein-glutamate O-methyltransferase {ECO:0000256|ARBA:ARBA00012534};
DE EC=2.1.1.80 {ECO:0000256|ARBA:ARBA00012534};
GN ORFNames=MSLAZ_1639 {ECO:0000313|EMBL:AKB74900.1};
OS Methanosarcina lacustris Z-7289.
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=1434111 {ECO:0000313|EMBL:AKB74900.1, ECO:0000313|Proteomes:UP000033072};
RN [1] {ECO:0000313|EMBL:AKB74900.1, ECO:0000313|Proteomes:UP000033072}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Z-7289 {ECO:0000313|EMBL:AKB74900.1,
RC ECO:0000313|Proteomes:UP000033072};
RA Henriksen J.R., Luke J., Reinhart S., Benedict M.N., Youngblut N.D.,
RA Metcalf M.E., Whitaker R.J., Metcalf W.W.;
RT "Methanogenic archaea and the global carbon cycle.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-
CC glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:24452, Rhea:RHEA-COMP:10208, Rhea:RHEA-COMP:10311,
CC ChEBI:CHEBI:29973, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:82795; EC=2.1.1.80;
CC Evidence={ECO:0000256|ARBA:ARBA00001541};
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DR EMBL; CP009515; AKB74900.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0E3WTM3; -.
DR STRING; 1434111.MSLAZ_1639; -.
DR KEGG; mls:MSLAZ_1639; -.
DR PATRIC; fig|1434111.4.peg.2138; -.
DR HOGENOM; CLU_000892_0_1_2; -.
DR Proteomes; UP000033072; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro.
DR GO; GO:0008984; F:protein-glutamate methylesterase activity; IEA:InterPro.
DR GO; GO:0008983; F:protein-glutamate O-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR GO; GO:0034641; P:cellular nitrogen compound metabolic process; IEA:UniProt.
DR GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-UniRule.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:1901576; P:organic substance biosynthetic process; IEA:UniProt.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR CDD; cd16434; CheB-CheR_fusion; 1.
DR CDD; cd00130; PAS; 2.
DR Gene3D; 1.10.155.10; Chemotaxis receptor methyltransferase CheR, N-terminal domain; 1.
DR Gene3D; 3.40.50.180; Methylesterase CheB, C-terminal domain; 1.
DR Gene3D; 3.30.450.20; PAS domain; 2.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR035909; CheB_C.
DR InterPro; IPR022642; CheR_C.
DR InterPro; IPR000780; CheR_MeTrfase.
DR InterPro; IPR022641; CheR_N.
DR InterPro; IPR036804; CheR_N_sf.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR000700; PAS-assoc_C.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR013767; PAS_fold.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR000673; Sig_transdc_resp-reg_Me-estase.
DR NCBIfam; TIGR00229; sensory_box; 1.
DR PANTHER; PTHR24422; CHEMOTAXIS PROTEIN METHYLTRANSFERASE; 1.
DR PANTHER; PTHR24422:SF25; CHEMOTAXIS PROTEIN METHYLTRANSFERASE; 1.
DR Pfam; PF01339; CheB_methylest; 1.
DR Pfam; PF01739; CheR; 1.
DR Pfam; PF03705; CheR_N; 1.
DR Pfam; PF00989; PAS; 1.
DR Pfam; PF13596; PAS_10; 1.
DR PRINTS; PR00996; CHERMTFRASE.
DR SMART; SM00138; MeTrc; 1.
DR SMART; SM00091; PAS; 2.
DR SUPFAM; SSF47757; Chemotaxis receptor methyltransferase CheR, N-terminal domain; 1.
DR SUPFAM; SSF52738; Methylesterase CheB, C-terminal domain; 1.
DR SUPFAM; SSF55785; PYP-like sensor domain (PAS domain); 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS50122; CHEB; 1.
DR PROSITE; PS50123; CHER; 1.
DR PROSITE; PS50113; PAC; 1.
DR PROSITE; PS50112; PAS; 1.
PE 4: Predicted;
KW Chemotaxis {ECO:0000256|PROSITE-ProRule:PRU00050};
KW Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00050};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:AKB74900.1};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:AKB74900.1}.
FT DOMAIN 36..227
FT /note="CheB-type methylesterase"
FT /evidence="ECO:0000259|PROSITE:PS50122"
FT DOMAIN 230..506
FT /note="CheR-type methyltransferase"
FT /evidence="ECO:0000259|PROSITE:PS50123"
FT DOMAIN 840..890
FT /note="PAC"
FT /evidence="ECO:0000259|PROSITE:PS50113"
FT DOMAIN 893..936
FT /note="PAS"
FT /evidence="ECO:0000259|PROSITE:PS50112"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 703..729
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 703..718
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 48
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
FT ACT_SITE 77
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
FT ACT_SITE 169
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
SQ SEQUENCE 1017 AA; 113999 MW; 95D92D29B429C207 CRC64;
MIKKKEDTGK EGQVHSAEET AVNEQPAPDA DSNKIPKEDF PIVGIGASAG GLAAFEAFFS
AMPNDTNPGM AFVLVQHLDP NHKSLLTDLI GRYTRMPVYE IKDGMVVQPD CVYVIPPNHD
MIFQDGKLQL LEPSEPRGHR LPIDIFFRSL AQSKQELAIG IILSGTGSDG TLGVRAIKAE
GGMVMTQTPE SSEYDGMPRS AIATGLVDYP LPPVEMPAQL IAYVTKAFGK RPYSIPSTED
AMKNIFSLLR TQTGHDFSHY KQNTINRRIE RRMAVQNIQS VDEYVRYLEQ KPAEVEALFS
DLLIGVTSFF RNPTAFETLQ KKLIPDLFTN RRADSAIRIW VAGCSTGEEA YSIGILLQEQ
MEMLKKNFKV QIFATDIDSR AIGEARSGVY PASISIDISP ERLDRFFTQD SGGDYRIRKS
IRDMVVFSEQ DIIKDPPFSK LDMLSCRNVL IYMDRELQKK LIPLFHYALN PGGFLFLGPS
ETLGEFDNLF DMLDRKSKIY LKKYVDSGHP PMGIFNSPGL ESRTRETKRS SDKALVEIKP
QMRELTEQII LQNYAPVSVL VNQHGDILYL HGRTGMYLEP APGEAGMNIL KMAREGLRQE
LATGLYKVAV NKKPVFYPGL RVKTNGDFTT VNLTLRPVAA DPDAASGPDL FLVTFEKPPE
GEQSKTGKTT AIGAGEGAFE SAMEDDVRIL ELKRELQVKE EHLKASNEEL ETSNEELKSS
NEEMQSINEE LQSTNEELET SKEELQSVNE ELATVNTELQ NKVTDLSQAI NDMNNLLGGT
GIGTIFVDHQ LRILRFTPVV TQIINLIPTD VGRPVGHIVS NLLGYDRLVI DIKEVLDNLI
PKDIEVQTQD GSWYLLRIRP YRTLENVIKG AVITFTEISE MKRAREILKE SEVMHRLAVV
VQDASDAITL QDMEGHILAW NPKAENMYGW SEAEALKMNI SSLVPASRKE GELVTLKKLS
HSEVMEPYRT QRLAEDGRIM DVWLTATSLV NKDNEIYAIA TTEREIKPEN REKEIKE
//
