ID A0A0E9MMT2_9SPHN Unreviewed; 756 AA.
AC A0A0E9MMT2;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=phosphoenolpyruvate--protein phosphotransferase {ECO:0000256|ARBA:ARBA00012232};
DE EC=2.7.3.9 {ECO:0000256|ARBA:ARBA00012232};
GN Name=ptsP {ECO:0000313|EMBL:GAO39082.1};
GN ORFNames=SCH01S_25_00620 {ECO:0000313|EMBL:GAO39082.1};
OS Sphingomonas changbaiensis NBRC 104936.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingomonas.
OX NCBI_TaxID=1219043 {ECO:0000313|EMBL:GAO39082.1, ECO:0000313|Proteomes:UP000033202};
RN [1] {ECO:0000313|EMBL:GAO39082.1, ECO:0000313|Proteomes:UP000033202}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 104936 {ECO:0000313|EMBL:GAO39082.1,
RC ECO:0000313|Proteomes:UP000033202};
RA Katano-Makiyama Y., Hosoyama A., Hashimoto M., Noguchi M., Tsuchikane K.,
RA Ohji S., Yamazoe A., Ichikawa N., Kimura A., Fujita N.;
RT "Whole genome shotgun sequence of Sphingomonas changbaiensis NBRC 104936.";
RL Submitted (APR-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-
CC L-histidyl-[protein] + pyruvate; Xref=Rhea:RHEA:23880, Rhea:RHEA-
CC COMP:9745, Rhea:RHEA-COMP:9746, ChEBI:CHEBI:15361, ChEBI:CHEBI:29979,
CC ChEBI:CHEBI:58702, ChEBI:CHEBI:64837; EC=2.7.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00000683};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the PEP-utilizing enzyme family.
CC {ECO:0000256|ARBA:ARBA00007837}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAO39082.1}.
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DR EMBL; BBWU01000025; GAO39082.1; -; Genomic_DNA.
DR RefSeq; WP_046347910.1; NZ_BBWU01000025.1.
DR AlphaFoldDB; A0A0E9MMT2; -.
DR STRING; 1219043.SCH01S_25_00620; -.
DR OrthoDB; 9765468at2; -.
DR Proteomes; UP000033202; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:InterPro.
DR Gene3D; 3.30.450.40; -; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR Gene3D; 3.50.30.10; Phosphohistidine domain; 1.
DR Gene3D; 1.10.274.10; PtsI, HPr-binding domain; 1.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR008279; PEP-util_enz_mobile_dom.
DR InterPro; IPR000121; PEP_util_C.
DR InterPro; IPR023151; PEP_util_CS.
DR InterPro; IPR036637; Phosphohistidine_dom_sf.
DR InterPro; IPR006318; PTS_EI-like.
DR InterPro; IPR008731; PTS_EIN.
DR InterPro; IPR036618; PtsI_HPr-bd_sf.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR NCBIfam; TIGR01417; PTS_I_fam; 1.
DR PANTHER; PTHR46244; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR PANTHER; PTHR46244:SF3; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF05524; PEP-utilisers_N; 1.
DR Pfam; PF00391; PEP-utilizers; 1.
DR Pfam; PF02896; PEP-utilizers_C; 1.
DR PRINTS; PR01736; PHPHTRNFRASE.
DR SMART; SM00065; GAF; 1.
DR SUPFAM; SSF47831; Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain; 1.
DR SUPFAM; SSF55781; GAF domain-like; 1.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF52009; Phosphohistidine domain; 1.
DR PROSITE; PS00742; PEP_ENZYMES_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Pyruvate {ECO:0000313|EMBL:GAO39082.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000033202};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:GAO39082.1};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 25..170
FT /note="GAF"
FT /evidence="ECO:0000259|SMART:SM00065"
SQ SEQUENCE 756 AA; 83417 MW; D33C5AD7FF3EB87A CRC64;
MAVSTASSAR EILTRLHDLM ASRANAQTKL NQVVGIIGEA TSSEVCSVYL LRDGALELFA
TRGLKEEAVH VTKLALGEGL VGSIAERVEM LNLDEAAAHP DFAYKPETGE ERFHSFAGVP
IIRRERAVGV LCVQHTEPRR YEDVEIEALQ TVAMVLSELI ANAGLIDGPA VEARPASTAA
LRMGGQKLVD GMARGIAVYH QPRITIEHTV AEDIEAERRR VYSAFTKMRE QIDSMAAQAE
FGVGGEHDEV LETYKMFAYD EGWSRRINEA IDSGLTAEAA IERVQQRTRM RMRQIDDPLL
ADRMHDLEDL SNRLIRIVSG QLGTAAQMGL RQDAILIARN LGPAELLEYD RRRLKGVILE
EGSLTAHVTI VARAMGVPVL GRVHNVRRLI GEGDHLLLDA SQSAVYVRPS PSMEEAFEAK
LSSSAKRRAA YAALRGLPPI TKDGQRITVM VNAGLRDDAA ALDLTGADGI GLFRTEFQFL
VSATLPQRER QQRLYRDVLD AARDRPVVFR TVDIGGDKAL PYMRAEELGE ENPAMGWRAL
RVALERNGLM KAQARALLEA AAGRTLSVMF PMVSEPWEFD AAKKIFDAQR DWIASRHRPL
PAQIRYGAML EVPALAECLD LLLPRLDFLS IGTNDLTQFL FAADRAHPKL AERYDWLSPA
ILRFLRRVSQ PAKERGIPVT VCGEMGGRTL EAMALLGLGI ERLSITPIAV GPIKAMVRSL
DLAKLRAEMD GWLVRPPANL RETLLSWANA HAVELA
//
