ID   A0A0F0L934_9MICO        Unreviewed;       736 AA.
AC   A0A0F0L934;
DT   24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT   24-JUN-2015, sequence version 1.
DT   27-MAR-2024, entry version 43.
DE   SubName: Full=Glycogen debranching enzyme {ECO:0000313|EMBL:KJL29663.1};
DE            EC=3.2.1.- {ECO:0000313|EMBL:KJL29663.1};
GN   Name=glgX_1 {ECO:0000313|EMBL:KJL29663.1};
GN   ORFNames=RS83_01685 {ECO:0000313|EMBL:KJL29663.1};
OS   Microbacterium oxydans.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Microbacterium.
OX   NCBI_TaxID=82380 {ECO:0000313|EMBL:KJL29663.1, ECO:0000313|Proteomes:UP000033640};
RN   [1] {ECO:0000313|EMBL:KJL29663.1, ECO:0000313|Proteomes:UP000033640}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BEL4b {ECO:0000313|EMBL:KJL29663.1,
RC   ECO:0000313|Proteomes:UP000033640};
RA   Corretto E.;
RT   "Draft genome sequences of ten Microbacterium spp. with emphasis on heavy
RT   metal contaminated environments.";
RL   Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KJL29663.1}.
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DR   EMBL; JYIW01000023; KJL29663.1; -; Genomic_DNA.
DR   RefSeq; WP_045279046.1; NZ_JYIW01000023.1.
DR   AlphaFoldDB; A0A0F0L934; -.
DR   PATRIC; fig|82380.11.peg.1722; -.
DR   OrthoDB; 3236218at2; -.
DR   Proteomes; UP000033640; Unassembled WGS sequence.
DR   GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR011837; Glycogen_debranch_GlgX.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   NCBIfam; TIGR02100; glgX_debranch; 1.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 2.
DR   Pfam; PF02922; CBM_48; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000313|EMBL:KJL29663.1};
KW   Hydrolase {ECO:0000313|EMBL:KJL29663.1}.
FT   DOMAIN          156..567
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   REGION          465..494
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        465..479
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   736 AA;  82263 MW;  4104A2E96090AF89 CRC64;
     MSHEVWPGSP YPLGATFDGQ GTNFALFSEG AERVELCLFD EDGSEERVPL TEFDAFVWHG
     FLPSVQPGQL YGYRVYGPDK PTEGKRYNPH KLLLDPYAKA VSGQIEWGQS LFGYDFGDPD
     SRNEEDSAAA MVKGVVINPF FEWAGDRLPK TPYAQTVIYE SHVKGLTFRH PDIPEELRGT
     YAGIAHPAII EHLVHLGITA IELMPVHQFV HDSTLEEKGL SNYWGYNTLA FFAPHSEYSS
     SGKLGQQVQE FKAMVRALHA AGIEVILDVV YNHTAEGNHL GPTLSMRGID NEAYYRLEED
     RRYYTDYTGT GNSLNAGSPH ALQLIMDSLR YWVTEMHVDG FRFDLASTLA REFYDVDKLS
     TFFELVQQDP VVSQVKLIAE PWDVGPGGYQ VGNFPPQWTE WNGKYRDTVR DFWRGEPQAL
     GEFASRLTGS ADLYEHSGRR PVASINFVTA HDGFTLRDLV SYNEKHNDAN GEDNNDGESH
     NRSSNMGVEG PTDDESINRA RARQQRNFLA TLLLSQGVPM ISHGDELGRT QGGNNNGYAQ
     DNEITWVDWD AADHPLIEFT AAVTRLRRTH PTFRRSRFFD GRPVRAEDGE RIPDVVWLRP
     DAVPMAPEDW DSGFGLSVGM FLNGHGIREK DLRGRPVTDQ NFLVYFNSGD EAVDLTLPDD
     RHGDSWQIMI DTAGERGNES AVPAGDSIEL QAKSLLVLCE AEPVEIVVDD SVNASLRVQS
     EQSVLSAPAP TAEHRA
//