ID A0A0F3IYU3_9PROT Unreviewed; 890 AA.
AC A0A0F3IYU3;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=Aconitate hydratase {ECO:0000256|RuleBase:RU361275};
DE Short=Aconitase {ECO:0000256|RuleBase:RU361275};
DE EC=4.2.1.3 {ECO:0000256|RuleBase:RU361275};
GN ORFNames=VZ95_02765 {ECO:0000313|EMBL:KJV10769.1};
OS Elstera litoralis.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Rhodospirillaceae; Elstera.
OX NCBI_TaxID=552518 {ECO:0000313|EMBL:KJV10769.1, ECO:0000313|Proteomes:UP000033774};
RN [1] {ECO:0000313|EMBL:KJV10769.1, ECO:0000313|Proteomes:UP000033774}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Dia-1 {ECO:0000313|EMBL:KJV10769.1,
RC ECO:0000313|Proteomes:UP000033774};
RA Rahalkar M.C., Dhakephalkar P.K., Pore S.D., Arora P., Kapse N.G.,
RA Pandit P.S.;
RT "Draft genome sequence of Elstera litoralis.";
RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the isomerization of citrate to isocitrate via cis-
CC aconitate. {ECO:0000256|RuleBase:RU361275}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501,
CC ECO:0000256|RuleBase:RU361275};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185, ECO:0000256|RuleBase:RU361275}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KJV10769.1}.
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DR EMBL; LAJY01000049; KJV10769.1; -; Genomic_DNA.
DR RefSeq; WP_045774518.1; NZ_LAJY01000049.1.
DR AlphaFoldDB; A0A0F3IYU3; -.
DR PATRIC; fig|552518.3.peg.3163; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000033774; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd01586; AcnA_IRP; 1.
DR CDD; cd01580; AcnA_IRP_Swivel; 1.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR044137; AcnA_IRP_Swivel.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01341; aconitase_1; 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR PANTHER; PTHR11670:SF54; CYTOPLASMIC ACONITATE HYDRATASE; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|RuleBase:RU361275};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU361275};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|RuleBase:RU361275};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|RuleBase:RU361275};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000033774}.
FT DOMAIN 76..559
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 694..816
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 890 AA; 95687 MW; 5AF908CAF0C50C14 CRC64;
MTVGIDTLKT RRTLSVDGVS FDYFSLAALV EAGHTNVTRL PMSLKVLLEN LLRYQDERTV
SVADVKSLAD WQPSGEEKEI AYRPARVLMQ DFTGVPAVVD LAAMRDAMVA LGGDPQKINP
LSPVDLVIDH SVMVDNFGTE HAFADNVKLE FERNHERYAF LRWGQSAFNN FRVVPPGTGI
CHQVNVEYLA QVVWTATEDG KTVAYPDTCV GTDSHTTMVN GLAVLGWGVG GIEAEAAMLG
QPVSMLVPDV VGFKLTGKLP EGATATDLVL TVTQMLRKLG VVGKFVEFYG PGLDHLSLAD
RSTIANMAPE YGATCGFFPI DAETIRYLNF TGREPARVKL VEAYAKAQGM WRDATTPDPV
FTTTLELDLA SVLPSMAGPK RPQDRVLLSD ANTSFSKTLT DSGVPAVRKV AVEGSNYELT
DGDVVITAIT SCTNTSNPSV LIAAGLLAKK AVELGLERKP WVKSSLAPGS QVVTDYLDKA
GLTPYLDKLG FNLVGYGCTT CIGNSGPLAD PIAAAVEKGD LTVAAVLSGN RNFEGRISPH
VKANYLASPP LVVAYALAGT MTVNVATDPL GTGKDGQPVY LKDIWPSNAD IQAALEASLT
SEMFKARYGN VFHGPDEWRA IETTTGETYV WDGGSTYVQN PPYFVGMAKE PGAVSDIRKA
RPLAILGDSI TTDHISPAGS IKKDSPGGAY LLEHQVRPID FNSYGARRGN HEVMMRGTFA
NIRLKNEMAP GTSGGVTVFQ PTGEPMFIYD AAQKYKAEGT PLVIVAGKEY GTGSSRDWAA
KGTLLLGVKA VIAESFERIH RSNLVGMGIL PLQFKDGMTR QTLGLDGTEV FDISGVEAGL
KPRQDLTVTL TRANGTVEVI QVLSRIDTLD EVEYYRHGGI LPYVLRSLAS
//
