ID A0A0F4JLE9_9ACTN Unreviewed; 498 AA.
AC A0A0F4JLE9;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Methionyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00030904};
DE Flags: Fragment;
GN ORFNames=VR44_10965 {ECO:0000313|EMBL:KJY35005.1};
OS Streptomyces katrae.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=68223 {ECO:0000313|EMBL:KJY35005.1, ECO:0000313|Proteomes:UP000033551};
RN [1] {ECO:0000313|EMBL:KJY35005.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL ISP-5550 {ECO:0000313|EMBL:KJY35005.1};
RA Ju K.-S., Doroghazi J.R., Metcalf W.;
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-
CC methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667,
CC Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530,
CC ChEBI:CHEBI:456215; EC=6.1.1.10;
CC Evidence={ECO:0000256|ARBA:ARBA00001234};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|RuleBase:RU363039}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KJY35005.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JZWV01000248; KJY35005.1; -; Genomic_DNA.
DR RefSeq; WP_045947241.1; NZ_JZWV01000248.1.
DR STRING; 68223.GCA_002028425_01107; -.
DR OrthoDB; 9810191at2; -.
DR Proteomes; UP000033551; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:InterPro.
DR GO; GO:0006418; P:tRNA aminoacylation for protein translation; IEA:InterPro.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 2.20.28.20; Methionyl-tRNA synthetase, Zn-domain; 1.
DR InterPro; IPR023458; Met-tRNA_ligase_1.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR029038; MetRS_Zn.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR45765; METHIONINE--TRNA LIGASE; 1.
DR PANTHER; PTHR45765:SF1; METHIONINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363039};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363039};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363039};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363039};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363039}.
FT DOMAIN 6..371
FT /note="Methionyl/Leucyl tRNA synthetase"
FT /evidence="ECO:0000259|Pfam:PF09334"
FT NON_TER 498
FT /evidence="ECO:0000313|EMBL:KJY35005.1"
SQ SEQUENCE 498 AA; 54548 MW; 6FA7CE448102DC9D CRC64;
MSTPVWITAT PPATHGELHV GHLAGPYVAA DVLSRYLRAE GEPVLFTTGT ADHNSSVHAR
ALRAGRKPEE VAEGYRAAIT SDWLRSGVEF DHVVRPRQDK GYGRWLADLF RRLHAEGVIA
ARTRLTPYCE SCERWLHGAH VRGTCPHCAA PSDGGMCHEC ARPNDGGDLL EPRCAQCGST
AVPRRCRRLY VPLEPHRDAL AEYWAGTGLP PRLAALCESL VEDGLPDIAV AHPAEWGLPV
PVDGFPEHRI DGCFEAAAMH LFGYGDQALP ERAIHFCGFG HAFCHAVLLP VLLFAQGVKL
PQHFCVNEAY VVEDEALVWG SGQKAWALDL ITEYGSDTLR RHVLQARPLG RRTEFRPERL
AAARRELDES WNSWLSRLFA AVREECAGLA PAALPGGAGW EVLERRLLRG VEDLREAYSP
ESFDPRRAVA LLDEMVRSAA DFGHVNAHEA HRPTADSRHL PALAAQLAVA RALGAWARPV
MPEGADRLSA ALKAQPXG
//
