ID A0A0F7GBW7_9CHLR Unreviewed; 489 AA.
AC A0A0F7GBW7;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:AKG52812.1};
GN ORFNames=DGWBC_0122 {ECO:0000313|EMBL:AKG52812.1};
OS Dehalogenimonas sp. WBC-2.
OC Bacteria; Chloroflexota; Dehalococcoidia; Dehalogenimonas.
OX NCBI_TaxID=943347 {ECO:0000313|EMBL:AKG52812.1, ECO:0000313|Proteomes:UP000034106};
RN [1] {ECO:0000313|EMBL:AKG52812.1, ECO:0000313|Proteomes:UP000034106}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WBC-2 {ECO:0000313|EMBL:AKG52812.1,
RC ECO:0000313|Proteomes:UP000034106};
RX PubMed=26452554; DOI=10.1128/AEM.02017-15;
RA Molenda O., Quaile A.T., Edwards E.A.;
RT "Dehalogenimonas sp. Strain WBC-2 Genome and Identification of Its trans-
RT Dichloroethene Reductive Dehalogenase, TdrA.";
RL Appl. Environ. Microbiol. 82:40-50(2015).
RN [2] {ECO:0000313|Proteomes:UP000034106}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WBC-2 {ECO:0000313|Proteomes:UP000034106};
RA Molenda O., Edwards E.A.;
RT "Identification of trans-1,2-dichloroethene reductive dehalogenase (TdrA)
RT in Dehalogenimonas sp. WBC-2.";
RL Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; CP011392; AKG52812.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0F7GBW7; -.
DR STRING; 943347.DGWBC_0122; -.
DR KEGG; dew:DGWBC_0122; -.
DR PATRIC; fig|943347.4.peg.120; -.
DR Proteomes; UP000034106; Chromosome.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409:SF7; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485}.
FT DOMAIN 1..132
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 169..397
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 489 AA; 55472 MW; 48724B7F1BEE06D4 CRC64;
MRILLSIPLR DGVYTKFPDE LLSIAAVLEQ HGHEVMLHDS NLGDRTAESF IDFNPSIVGF
SVSTGCIADS IKKSVEFKKV FPGIKTAWGF RHPSALPEQT LIEKYVDYVI IGAGEYTLLE
LAEYIEKGIG RLADIKGLAW KSGGDIVINE PRPFLKNLDE LPDPAWHLVD PKKYWDVSII
TSRGCPYTCT FCADATFYKG NVTDLSAERI ASQSERMHKE FGINYIMYTG DNFGINKPRL
HEFCHIMIKK KLSLKWNCQI SGNVTQEDAK LMSRAGCTAV ILGAESGSQK ILDLLTKGSV
KEFEQTFWNL VKQRIIPTLF IQYGFPTETA ADFKETLDFI KRLDNPPYLF MKFVPYPKTV
LFDQCVDKKL VTVPSSLADW SGFQFYHATQ ANLSRIPDKL MDEAMANFRT TFATRRFRFM
LRHNPGYFIN AIKAPKEFWG SLSYLIKNYL NALMDAANGK ESWLVRFRRA LGMKDAKQKS
SSVLAAKET
//
