ID A0A0G1U5L4_9BACT Unreviewed; 691 AA.
AC A0A0G1U5L4;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=Isoleucine-tRNA ligase {ECO:0000313|EMBL:KKU89377.1};
GN ORFNames=UY19_C0015G0017 {ECO:0000313|EMBL:KKU89377.1};
OS Candidatus Wolfebacteria bacterium GW2011_GWA2_47_9b.
OC Bacteria; Candidatus Wolfebacteria.
OX NCBI_TaxID=1619005 {ECO:0000313|EMBL:KKU89377.1, ECO:0000313|Proteomes:UP000033882};
RN [1] {ECO:0000313|EMBL:KKU89377.1, ECO:0000313|Proteomes:UP000033882}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Brown C.T., Hug L.A., Thomas B.C., Sharon I., Castelle C.J., Singh A.,
RA Wilkins M.J., Williams K.H., Banfield J.F.;
RT "rRNA introns, odd ribosomes, and small enigmatic genomes across a large
RT radiation of phyla.";
RL Nature 0:0-0(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KKU89377.1}.
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DR EMBL; LCPB01000015; KKU89377.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0G1U5L4; -.
DR PATRIC; fig|1619005.3.peg.862; -.
DR Proteomes; UP000033882; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:InterPro.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006418; P:tRNA aminoacylation for protein translation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd07067; HP_PGM_like; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 3.40.50.1240; Phosphoglycerate mutase-like; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR013078; His_Pase_superF_clade-1.
DR InterPro; IPR029033; His_PPase_superfam.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00300; His_Phos_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR SMART; SM00855; PGAM; 1.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF53254; Phosphoglycerate mutase-like; 1.
PE 4: Predicted;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:KKU89377.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917}.
FT DOMAIN 220..357
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 406..554
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT BINDING 23..30
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR613078-2"
FT BINDING 79
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR613078-2"
SQ SEQUENCE 691 AA; 78016 MW; 4F0D0ABCA86ADDF6 CRC64;
MVGSVAEIKQ NTPKSGNMYF TLRHGQSENN ALNLVSSNPK NTYHLTEKGK GQVARSTKAF
AKQLKGKKID VIFSSDYARA QETAEIAAKT LGYEAKIIID KRLREINCGI FDNRPISEYH
AYFASLEEKF AKVAPKGESL TDVKKRMTEF VYDIDAKYKG KNILIVSHEY PIWALFAGVQ
GFDGPKAVAM RKGNKDFVLN AEIKKLDFSI IPHNKNYELD LHRPYIDRVD LVCTKGHAMK
RVPDVFDCWF ESGSMPYGQA HYPFEGKKKF EKNFPAEFIA EAVDQTRGWF YTLMVLSTGL
FGKPAFKNVF ATGLVLAEDG QKMSKKLKNY PDPMTIVDKY GADALRLYLV SSPIVRGEVL
NFSEKGVDEL YKKVISRLWN VYSFYDMYGQ QQKVIARPKG RVTELDKWML GRLDELVAEV
TGAMGKYELD RAVRPIGQFV DDLSTWYVRR SRRRFQKPDD KKDWELASKT LAYILMETSK
VLAPFTPFFT DALYKSLDQK KNASVHLSAW PKSAALAVLK TNKKMGVMMA EVRNLASIAL
AKRASLGIKV RQPLATLTVQ SSVVGLKTNK ELLAILADEV NVKKIVVKAN VEGIVEFDTT
ITPALLEEGI VRESVRMVQG LRQDAGYEPK DRILLFVDSA ALGDVMKKYE DLLKREVGAK
AVVFAPEAEH LDAYAELVLD QDRIWFGLRK A
//
