ID A0A0G2K481_RAT Unreviewed; 2079 AA.
AC A0A0G2K481;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 25-MAY-2022, sequence version 2.
DT 27-MAR-2024, entry version 40.
DE SubName: Full=Sperm flagellar 2 {ECO:0000313|Ensembl:ENSRNOP00000072940.2};
GN Name=Spef2 {ECO:0000313|Ensembl:ENSRNOP00000072940.2,
GN ECO:0000313|RGD:620450};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116 {ECO:0000313|Ensembl:ENSRNOP00000072940.2, ECO:0000313|Proteomes:UP000002494};
RN [1] {ECO:0000313|Ensembl:ENSRNOP00000072940.2, ECO:0000313|Proteomes:UP000002494}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000072940.2,
RC ECO:0000313|Proteomes:UP000002494};
RX PubMed=15057822; DOI=10.1038/nature02426;
RG Rat Genome Sequencing Project Consortium;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2] {ECO:0007829|PubMed:22673903}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
RN [3] {ECO:0000313|Ensembl:ENSRNOP00000072940.2}
RP IDENTIFICATION.
RC STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000072940.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
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DR jPOST; A0A0G2K481; -.
DR Ensembl; ENSRNOT00000088701.2; ENSRNOP00000072940.2; ENSRNOG00000058275.2.
DR RGD; 620450; Spef2.
DR VEuPathDB; HostDB:ENSRNOG00000058275; -.
DR GeneTree; ENSGT00390000008160; -.
DR Proteomes; UP000002494; Chromosome 2.
DR Bgee; ENSRNOG00000058275; Expressed in testis and 4 other cell types or tissues.
DR ExpressionAtlas; A0A0G2K481; baseline and differential.
DR GO; GO:0036126; C:sperm flagellum; IEA:Ensembl.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0007288; P:sperm axoneme assembly; IEA:Ensembl.
DR Gene3D; 1.10.418.10; Calponin-like domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR010441; CH_2.
DR InterPro; IPR001715; CH_dom.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR008906; HATC_C_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR012337; RNaseH-like_sf.
DR PANTHER; PTHR14919; KPL2-RELATED; 1.
DR PANTHER; PTHR14919:SF0; SPERM FLAGELLAR PROTEIN 2; 1.
DR Pfam; PF00406; ADK; 1.
DR Pfam; PF06294; CH_2; 1.
DR Pfam; PF05699; Dimer_Tnp_hAT; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS50021; CH; 1.
PE 1: Evidence at protein level;
KW Reference proteome {ECO:0000313|Proteomes:UP000002494}.
FT DOMAIN 1..105
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000259|PROSITE:PS50021"
FT REGION 547..602
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 826..892
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1176..1227
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2047..2079
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 551..576
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 577..602
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1176..1222
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2047..2065
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2079 AA; 238610 MW; E764D681402C58AC CRC64;
MSEILCQWLN QELKVSRTVS PKSFAKTFSN GYLIGEVLFK FELQSDFSEF SESRVSTAKL
NNFSRLQPTL HLLGVQFDQN VAQSIITEKP GAATKLLYQL YIALQKKKKT GLTGLEIQTM
QPQTNQRLQA LKSEAFREQR LNRRRQNEIM AKIQAAIIQI PKPEPNRTLK AIEAQKLMKK
KKEAEDVANE IKKFEALIKK DLQTRESISK TSLDTIDQTA AELLNTYSDD DYIKKIQKRL
EEDAFAREQR EKRRRRLLMD QLIAHEAQEE AYREEQLIHR LMRQSQQERR IAVQLMHVRH
EKEVLWQNRI FREKQFEERR LKDFQDALDR EAALTKQAKI DFAEQTLRAK EIHEKIAMER
AQQRYEKHYG ICAEILDQIL DLCTKVADYR LLTNNLIPHK MMRDWKELFF SGNPIYEQTS
HTHGQTESTE DHHVELNKRN LLDSKDYEDY KNMTGEWALP EDMANSSPPS NNNILGHVLL
RLIENSYPSE KEVADTELPS FAVKGCLLGK TLSGKTTILK ALQNDIPIQV LSIDTLVQEA
IQAFNDGEKS SKAPSMHEDV KGDPEVDQKE MIIPQDKNEL TDTQVPGEAT PQKEGTKSSD
FEQFRPSNSF LSLSMRAQLG AKSELMLRRG KSVPDVLLVN ILVNAIKKIP VNQSWVLDGF
PMTVNQAKLL EEALTGYKRK SLQLKKKKAQ MPTLALSPTT SKEVPLLPSA FDFAILLDIS
DNSSLARIND IIAKEISHEI SHENVSGPTI TSSEEKSEDR NLRDHIQHRI VGFLDNWPLL
EEWFTEPRNI LKKVNAEIDE ALLCQKIKEI FATEVSNKKI KVEKKLEEKE TEKKSAVSPT
EPPTPTPPLA SEAEKEKEVH QAKPSGKGKT QSVSPKGKAQ GGKISVKKSP IDSTEVSPTL
IAMPTTKPGS EEWVYVTEPI PEELPSFLVP YWELIEKTYI SHIKTVLRQL RESQHTVLSY
LYETRTSFQE FLRRPDHKQD FVSQWQADFN SVPEDLWEDE ETKAELHQRV NDLRDRLWDI
CEARKEEAEQ ERLDIINESW LQDYIGITMN HFFSLMQAEV NRFQDTKRLL QDYYRAMESK
IPLEDNKKFT RIPLVQLDGK EISEHQLRIP LVPRISSSPE NVSMRPKVKA LLKGRFDPPL
ESLEANFEGD EKIVLDTWQQ ASLAISQMVA AEVHQRLAEE EKEQPQLDPK EKSPQSGANK
KAKKEKEPPK KKMAEKKGKG KSSPVVEVSP VTITPEEMAE MERKNELKLK IKEEHLAALQ
TEEQATQFRL ELIKSKALSI LEDLVTKVIE VYRFMEKWLG KRYLNEMASI QKLTEVARYH
IETATKIQNE IYLSQEDFYI NGDIKVFPDP PPPTRPPPVE KEENGTLTIE QLDNLRDQFL
DMAPKGKDFP DAVISVTKKQ KKSRKVDSYL PERNHQFLKI WLKKYPWLVY DELLNLMFCV
LCRKHGVMSG GSTVSFFYGT DNFRAEFLSA HHFSEAHAKA SLMEATSDSP VNRAATELMV
RTMSKVTLGR VENLFRSCHA LVKTGHSLRD FIWMCKLDDM KGVDIGPVFR TNKSARTFTY
FIAEVERKNL REKLEKSNFF SVITDGTVDS LGKEAKMVYV QFAHAGKVQC QIVGIQPVER
KDPLTIKNAI EKTLETNLHL KLSSQDLVKK LVGFGSDDYP GIEGENRVSL LLREMQPCVQ
SMYCFAHHLE LSYREMFQTV PLYNNLEALL TDIYHFYHNS PLLKSSLITA FRGLHLQPVM
PSQIRGRRWF HGMQAALQNF LKGYPAIAQQ LHSVGKGKDD TSQQKANRLL ELILRADIIK
FAHFLTDILS ILSLLSHASR NRNSSIADVS ASLESTLEML QIYQSRPGPK ERRVESTTHF
HGNSLRGKEN ISTVRKAVLT HLIERLRGCF RDASLDVVRA TVIGSFRLWP TKMNQEFGVK
EISILISHYQ PVLEAASVKT DEVHTEWSTL KLEIYARFQN IRKLTWDFVN STYSHKYPNV
LMLVDLVLAL PASSAEPERG WSQMKCTHSR MEEKIKTETM TDILTIQLNS PNISSFDPRK
AIHLWNSQTQ SRSAHRGPNL DSPSTSESED ESDEQCDLG
//
