ID A0A0G4J5G1_PLABS Unreviewed; 1041 AA.
AC A0A0G4J5G1;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=DNA replication ATP-dependent helicase/nuclease {ECO:0000256|RuleBase:RU367041};
DE EC=3.1.-.- {ECO:0000256|RuleBase:RU367041};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU367041};
GN ORFNames=PBRA_009125 {ECO:0000313|EMBL:CEP02541.1};
OS Plasmodiophora brassicae (Clubroot disease agent).
OC Eukaryota; Sar; Rhizaria; Endomyxa; Phytomyxea; Plasmodiophorida;
OC Plasmodiophoridae; Plasmodiophora.
OX NCBI_TaxID=37360 {ECO:0000313|EMBL:CEP02541.1, ECO:0000313|Proteomes:UP000039324};
RN [1] {ECO:0000313|EMBL:CEP02541.1, ECO:0000313|Proteomes:UP000039324}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=E3 {ECO:0000313|EMBL:CEP02541.1};
RA Chooi Y.-H.;
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Key enzyme involved in DNA replication and DNA repair.
CC Involved in Okazaki fragments processing by cleaving long flaps that
CC escape FEN1: flaps that are longer than 27 nucleotides are coated by
CC replication protein A complex (RPA), leading to recruit DNA2 which
CC cleaves the flap until it is too short to bind RPA and becomes a
CC substrate for FEN1. Also involved in 5'-end resection of DNA during
CC double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA.
CC {ECO:0000256|RuleBase:RU367041}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665,
CC ECO:0000256|RuleBase:RU367041};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU367041}.
CC Chromosome {ECO:0000256|RuleBase:RU367041}.
CC -!- SIMILARITY: Belongs to the DNA2/NAM7 helicase family.
CC {ECO:0000256|ARBA:ARBA00007913, ECO:0000256|RuleBase:RU367041}.
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DR EMBL; CDSF01000131; CEP02541.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0G4J5G1; -.
DR STRING; 37360.A0A0G4J5G1; -.
DR EnsemblProtists; CEP02541; CEP02541; PBRA_009125.
DR OMA; NYCEAAI; -.
DR OrthoDB; 170190at2759; -.
DR Proteomes; UP000039324; Unassembled WGS sequence.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0017108; F:5'-flap endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0017116; F:single-stranded DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0033567; P:DNA replication, Okazaki fragment processing; IEA:UniProtKB-UniRule.
DR CDD; cd18041; DEXXQc_DNA2; 1.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR026851; Dna2/JHS1_DEXXQ-box.
DR InterPro; IPR045055; DNA2/NAM7-like.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR014808; DNA_replication_fac_Dna2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR047187; SF1_C_Upf1.
DR PANTHER; PTHR10887:SF433; DNA REPLICATION ATP-DEPENDENT HELICASE_NUCLEASE DNA2; 1.
DR PANTHER; PTHR10887; DNA2/NAM7 HELICASE FAMILY; 1.
DR Pfam; PF13086; AAA_11; 1.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF08696; Dna2; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|RuleBase:RU367041};
KW ATP-binding {ECO:0000256|RuleBase:RU367041};
KW Chromosome {ECO:0000256|RuleBase:RU367041};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU367041};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU367041};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU367041};
KW DNA-binding {ECO:0000256|RuleBase:RU367041};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|RuleBase:RU367041};
KW Hydrolase {ECO:0000256|RuleBase:RU367041};
KW Iron {ECO:0000256|RuleBase:RU367041};
KW Iron-sulfur {ECO:0000256|RuleBase:RU367041};
KW Metal-binding {ECO:0000256|RuleBase:RU367041};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268,
KW ECO:0000256|RuleBase:RU367041}; Nuclease {ECO:0000256|RuleBase:RU367041};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU367041};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU367041};
KW Reference proteome {ECO:0000313|Proteomes:UP000039324}.
FT DOMAIN 47..246
FT /note="DNA replication factor Dna2 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08696"
FT DOMAIN 697..763
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 773..981
FT /note="DNA2/NAM7 helicase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13087"
SQ SEQUENCE 1041 AA; 116041 MW; 8E9702DD291E5D2D CRC64;
MGLLDAEFDA ALVALGDLEL PAPCTRFLVL EAGVDSAGTV LRLFHEGHGA ERVCRVRGDW
RECFIDVGDY VNVIGEAGSD GEYVVDNENN MIIVHPDRLV SGTRVGSSVA CMRRAVLSER
VNAPSSSVKA IRGTLAHQLF QACLRSGNFS ERFARDQVAD VVRDAYLDLI LFEETEQDTV
HYLTSLIPQL QAWSSTFCGR AASADSEVHF ADKRVSTRIV DVLDIEDQVL APRYGLQGKI
DATVRAECRE LGPLSAAVPR VLPLELKTGK PRTADNAQLS LYTLLLSERY SPERVESGLL
LYLTAQSVAM KGASRTGLVC SSVRMTRLVG IVHRHGELRQ LIIKRNIIAS YAVGSEMHGR
SGALPALIDN DHYCRNCFQA DSCMLFRKAY ESSDDRDPHP LDDIVGHLNE NHMRYFRFWN
QMIDLDSREA RQRQSRIWTE TSATCQAHGR CVSNVCLDSE QVIGEMFFCT FKASLDSTTN
FTSLYFEEGD KVAISTEDGQ VGIATGFVVN VDERSVVVRI YNRRLSVRFQ HCRFRIDKDE
YISRYANLRG NLVALCAQAP DSDRLRDLIV DLRSPLFDIE RDLSVSDDDL RAAYFNDLNE
DQRKAVRHVM ATQDYALILG FPGTGKTTLI VFLVRLMVFL GQPVLLCCHT NTSLDNILQK
LVPIGVPFIR LGIMNRIQRS LHDHTLEEQR FQSLQEAREF CASQPVVGCT CLGASQTMLG
RRRFDWVICD EASQATEIAM LAPLRLGKRF VLVGDHYQLP PLVRDPVAKR SGMDTSLFLR
LSEAHPHAVV QLKSQYRMNE AILSLSNSLI YSNALVCGTD KVASARLHLP APKRLPRVTV
PGATFDWLGR ALDPERPVLF LNTDAVPAPD GHRGEQNETE AELVLALLDA LSGAGVPGSH
IGVISPYRSQ LQLLRRRLGA RRGDVEVESV DKFQGRDKDV IIMSLVRSNP KNRVGELLQD
WRRINVALTR SRYKLVLVGS QTTLRHSPIM AALIDVIHRQ HTVVDLPARA HLFYRQEEAD
EGAGAAVPQA KRLRLGPPGD G
//
