ID A0A0H1BN46_9EURO Unreviewed; 860 AA.
AC A0A0H1BN46;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 24-JAN-2024, entry version 28.
DE RecName: Full=Beta-mannosidase B {ECO:0000256|ARBA:ARBA00041069};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Mannanase B {ECO:0000256|ARBA:ARBA00041614};
GN ORFNames=EMPG_12545 {ECO:0000313|EMBL:KLJ12427.1};
OS Blastomyces silverae.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Onygenales; Ajellomycetaceae; Blastomyces.
OX NCBI_TaxID=2060906 {ECO:0000313|EMBL:KLJ12427.1, ECO:0000313|Proteomes:UP000053573};
RN [1] {ECO:0000313|Proteomes:UP000053573}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UAMH 139 {ECO:0000313|Proteomes:UP000053573};
RX PubMed=26439490; DOI=10.1371/journal.pgen.1005493;
RA Munoz J.F., Gauthier G.M., Desjardins C.A., Gallo J.E., Holder J.,
RA Sullivan T.D., Marty A.J., Carmen J.C., Chen Z., Ding L., Gujja S.,
RA Magrini V., Misas E., Mitreva M., Priest M., Saif S., Whiston E.A.,
RA Young S., Zeng Q., Goldman W.E., Mardis E.R., Taylor J.W., McEwen J.G.,
RA Clay O.K., Klein B.S., Cuomo C.A.;
RT "The dynamic genome and transcriptome of the human fungal pathogen
RT Blastomyces and close relative Emmonsia.";
RL PLoS Genet. 11:E1005493-E1005493(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC mannosidase B subfamily. {ECO:0000256|ARBA:ARBA00038429}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KLJ12427.1}.
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DR EMBL; LDEV01000926; KLJ12427.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0H1BN46; -.
DR STRING; 2060906.A0A0H1BN46; -.
DR OrthoDB; 2504097at2759; -.
DR UniPathway; UPA00280; -.
DR Proteomes; UP000053573; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000053573};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 195..297
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 685..782
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 791..843
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 860 AA; 98083 MW; 7C9AB8EEA1C48758 CRC64;
MSERSVIALN AGWVFKQADD EKAQFLSVSQ FPTNVHLDLM HHGLIPDPFI GKNELAVQWV
GEKSWVYQTS FDNPPLRDGE KAVLAFDGLD THATVLLNGK KILETENMFI PERVDVTELL
RESKGSNVLN ITFASTFLLG KKIKEQYPEH RWGCWNGDCS RLAVRKAQYH YGWDWGPTLM
TCGPWRPVHL EIYKARISDL HFRQNVDKSL KRAELVVTAE IEGNADNVAF HISLGGEEKG
SETVDIKDGI ASVTFHVQDP QLWYPSNYGE QPLYALSATL SSNRVKLDST TKRVGLRRAE
LIQRKMDDAE GTSFFFEVNN IPVFCGGSNW IPGDNFIPRI PPQKYYDWVK LMVAGNQMMV
RVWGGGLFEE QAFYDACDEL GVLVWQDFLF ACGNYPVFPN FLENVKREAI ENVKLLRHHP
SIVLWAGNNE DYQFAESENL DWDPSNNDPD SWLKSEFPAR YIYEKILVDV TKDLIPDTYY
HFGSPWGGKT SADPTVGDIH QWNVWHGTQE RYQDFDKLGG RFVSEFGLQG FPDIRTIDGY
LTGGKADPER HAQSSTVDFH NKAAGHERRT ALYLAENIKY NLEPLEQYIY ATQLIQAECL
SSAYRLWKRQ WKGPGREYCA GVLVWQMNDC WPVTSWALVD YHLRPKHAFY AVKRELASIT
IGLKRTSHTE YADKYTNVYY KTTHKVEMWA TNLTVKPRNV SVILKAWDVI TGREIYSRTL
HDRFVLEENR STEIAELEIP TNDEKEETKD GEEANRTVVA AYLFENGEQV ARYVNWPEPL
RYVHLQAPKE LQVALSADGK QVELSAEVPV KGVAVYADGD EVVFDDNCVD LVPGETVYIG
VKGLDKGQGD RISVKYLGSS
//
