ID A0A0J1DI05_9BURK Unreviewed; 388 AA.
AC A0A0J1DI05;
DT 14-OCT-2015, integrated into UniProtKB/TrEMBL.
DT 14-OCT-2015, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE SubName: Full=Mandelate racemase {ECO:0000313|EMBL:KLU38634.1};
GN ORFNames=AB595_01955 {ECO:0000313|EMBL:KLU38634.1};
OS Massilia sp. WF1.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Oxalobacteraceae; Telluria group; Massilia.
OX NCBI_TaxID=1406431 {ECO:0000313|EMBL:KLU38634.1, ECO:0000313|Proteomes:UP000036336};
RN [1] {ECO:0000313|Proteomes:UP000036336}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WF1 {ECO:0000313|Proteomes:UP000036336};
RA Lou J., Gu H., Wang H., Xu J.;
RT "High quality genome sequence of a efficient PHE-degrading Massilia sp.
RT strain WF1, isolated from a polycyclic aromatic hydrocarbons (PAHs)
RT contaminated soil.";
RL Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR634611-3};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR634611-3};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KLU38634.1}.
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DR EMBL; LELH02000001; KLU38634.1; -; Genomic_DNA.
DR RefSeq; WP_047821773.1; NZ_LELH02000001.1.
DR AlphaFoldDB; A0A0J1DI05; -.
DR PATRIC; fig|1406431.3.peg.406; -.
DR Proteomes; UP000036336; Unassembled WGS sequence.
DR GO; GO:0047808; F:D(-)-tartrate dehydratase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd03326; MR_like_1; 1.
DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR InterPro; IPR034611; D-tartrate_dehydratase.
DR InterPro; IPR034593; DgoD-like.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR029065; Enolase_C-like.
DR InterPro; IPR013342; Mandelate_racemase_C.
DR InterPro; IPR013341; Mandelate_racemase_N_dom.
DR PANTHER; PTHR48080:SF5; D(-)-TARTRATE DEHYDRATASE; 1.
DR PANTHER; PTHR48080; D-GALACTONATE DEHYDRATASE-RELATED; 1.
DR Pfam; PF13378; MR_MLE_C; 1.
DR Pfam; PF02746; MR_MLE_N; 1.
DR SFLD; SFLDF00118; D-tartrate_dehydratase; 1.
DR SFLD; SFLDG00179; mandelate_racemase; 1.
DR SMART; SM00922; MR_MLE; 1.
DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
PE 4: Predicted;
KW Magnesium {ECO:0000256|PIRSR:PIRSR634611-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR634611-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000036336}.
FT DOMAIN 162..259
FT /note="Mandelate racemase/muconate lactonizing enzyme C-
FT terminal"
FT /evidence="ECO:0000259|SMART:SM00922"
FT ACT_SITE 183
FT /note="acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-1"
FT ACT_SITE 321
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-1"
FT BINDING 19
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 53
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 100
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 155
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 181
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 212
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
FT BINDING 238
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
FT BINDING 238
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 264
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
FT BINDING 264
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 321
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT SITE 53
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
FT SITE 181
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
FT SITE 291
FT /note="Increases basicity of active site His"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
FT SITE 340
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
SQ SEQUENCE 388 AA; 43513 MW; A39610C98F2428F9 CRC64;
MKIVEIREQT LPISSPIRNA YIDFSKMTLS LVAVITDVIR DGKPVVGYGF NSNGRYGQGK
LMRERFIPRI LEADPASLVN DKGDNLDPHK IWDCMFTNEK PGGHGERSVA IGTIDMAVWD
AVAKIEGVPL FQLLADRYGN GQPDRKVFVY AAGGYYHPGQ DHEALKNEMR SYIDRGYTVV
KKKIGGASLD EDLRRIDSIL SVLQDGQKLA VDANGRFDLD TAIKYAKALS QYDLFWYEEA
GDPLDFELQA TLRNYYDKPM ATGENLFSMQ DARNLIRYGG MRPDRDWLQF DCALSYGLVE
YLRTLDMLHQ HGWSRKRCIP HGGHQMSLNI AAGLGLGGNE SYPDLFQPFG GFPDGVKVDN
GYLTMPDLPG IGFEGKADLY REMQKLSA
//
