ID A0A0J9EAW0_9RHOB Unreviewed; 730 AA.
AC A0A0J9EAW0;
DT 14-OCT-2015, integrated into UniProtKB/TrEMBL.
DT 14-OCT-2015, sequence version 1.
DT 24-JAN-2024, entry version 33.
DE SubName: Full=Enoyl-CoA hydratase {ECO:0000313|EMBL:KMW59761.1};
DE EC=1.1.1.35 {ECO:0000313|EMBL:KMW59761.1};
DE EC=4.2.1.17 {ECO:0000313|EMBL:KMW59761.1};
DE EC=5.1.2.3 {ECO:0000313|EMBL:KMW59761.1};
GN ORFNames=AIOL_004744 {ECO:0000313|EMBL:KMW59761.1};
OS Candidatus Rhodobacter lobularis.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Paracoccaceae; Rhodobacter.
OX NCBI_TaxID=1675527 {ECO:0000313|EMBL:KMW59761.1, ECO:0000313|Proteomes:UP000037178};
RN [1] {ECO:0000313|EMBL:KMW59761.1, ECO:0000313|Proteomes:UP000037178}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IGS {ECO:0000313|EMBL:KMW59761.1};
RA Jourda C., Santini S., Claverie J.-M.;
RT "Draft genome sequence of an Alphaproteobacteria species associated to the
RT Mediterranean sponge Oscarella lobularis.";
RL Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00023693};
CC -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC {ECO:0000256|ARBA:ARBA00005005}.
CC -!- SIMILARITY: In the central section; belongs to the 3-hydroxyacyl-CoA
CC dehydrogenase family. {ECO:0000256|ARBA:ARBA00007005}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KMW59761.1}.
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DR EMBL; LFTY01000002; KMW59761.1; -; Genomic_DNA.
DR RefSeq; WP_049645191.1; NZ_LFTY01000002.1.
DR AlphaFoldDB; A0A0J9EAW0; -.
DR STRING; 1675527.AIOL_004744; -.
DR PATRIC; fig|1675527.3.peg.4979; -.
DR OrthoDB; 9771883at2; -.
DR UniPathway; UPA00659; -.
DR Proteomes; UP000037178; Unassembled WGS sequence.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
DR CDD; cd06558; crotonase-like; 1.
DR Gene3D; 1.10.1040.50; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR InterPro; IPR006108; 3HC_DH_C.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR43612; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA; 1.
DR PANTHER; PTHR43612:SF3; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA, MITOCHONDRIAL; 1.
DR Pfam; PF00725; 3HCDH; 1.
DR Pfam; PF02737; 3HCDH_N; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 2.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Isomerase {ECO:0000313|EMBL:KMW59761.1};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:KMW59761.1};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000313|EMBL:KMW59761.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000037178}.
FT DOMAIN 327..505
FT /note="3-hydroxyacyl-CoA dehydrogenase NAD binding"
FT /evidence="ECO:0000259|Pfam:PF02737"
FT DOMAIN 509..604
FT /note="3-hydroxyacyl-CoA dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00725"
SQ SEQUENCE 730 AA; 77868 MW; 853F8CB72635E75C CRC64;
MADFIMTKGD DGVAVITWDV PGKSMNVMSR EAMPLLGNLV DDALADEAVT GIIITSGKKD
FAGGMDLNVL GSISAEAGDN PAQALFDFTM AGHHGLRKIE RAGMDPKTNK GGKPIACVLP
GTALGIGFEI PLACHRIFAA DNPKAKIGLP EILVGLFPGA GGTTRLVRKL GAMAASPFLL
EGKLSDPKKA KAAGLIDEVS EDPMAAAREW VLGATDADIV KPWDAKGYKM PGGMPYHPSG
FMTFVGAAAM VHGKTQGVYP AAKALLAAAY EGAQVPFETA LKIEARHFTS VLMSPTSSAM
IRSLFVNKEA LEKGANRPDV ADETVKKVGV LGAGMMGAGI ALVSAQAGIE VVLIDQTQEA
ADKGRAYTEA YMDKGIARRK ATPEKKEQML ARITATTDLS ALADCDLIVE AVFEDPAVKA
EMTKKVEAVI PADCIFASNT STLPITELAK ASSRPEQFIG IHFFSPVERM MLVEIIKGAE
TGDRAVAKSL DYVRQIRKTP IVVNDARFFY ANRCIIPYIN EGVRMVGEGV EPALVENAAK
MLGFPVGPLQ LNDETSIDLG VKIAKATKAA LGDAYDAGAA DEVLFWLFDQ GRMGRKSSAG
YYDYDEKGKR TGIWPGLRDK YTKDDHPDLT EVQHRLMMAQ VLEAVRAIEE DVLLDIREGD
VGAILGWGFA PWSGGPLSWL DMIGATKAVE ICDGLEAKFG KRFAAPALLK DIAAKGESFY
GRFTETKAAA
//
