ID   A0A0K9PNR8_ZOSMR        Unreviewed;       983 AA.
AC   A0A0K9PNR8;
DT   11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT   11-NOV-2015, sequence version 1.
DT   27-MAR-2024, entry version 28.
DE   SubName: Full=Ribonuclease E/G-like protein, chloroplastic {ECO:0000313|EMBL:KMZ70713.1};
GN   ORFNames=ZOSMA_195G00090 {ECO:0000313|EMBL:KMZ70713.1};
OS   Zostera marina (Eelgrass).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Zosteraceae; Zostera.
OX   NCBI_TaxID=29655 {ECO:0000313|EMBL:KMZ70713.1, ECO:0000313|Proteomes:UP000036987};
RN   [1] {ECO:0000313|Proteomes:UP000036987}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Finnish {ECO:0000313|Proteomes:UP000036987};
RX   PubMed=26814964; DOI=10.1038/nature16548;
RA   Olsen J.L., Rouze P., Verhelst B., Lin Y.-C., Bayer T., Collen J.,
RA   Dattolo E., De Paoli E., Dittami S., Maumus F., Michel G., Kersting A.,
RA   Lauritano C., Lohaus R., Toepel M., Tonon T., Vanneste K., Amirebrahimi M.,
RA   Brakel J., Bostroem C., Chovatia M., Grimwood J., Jenkins J.W.,
RA   Jueterbock A., Mraz A., Stam W.T., Tice H., Bornberg-Bauer E., Green P.J.,
RA   Pearson G.A., Procaccini G., Duarte C.M., Schmutz J., Reusch T.B.H.,
RA   Van de Peer Y.;
RT   "The genome of the seagrass Zostera marina reveals angiosperm adaptation to
RT   the sea.";
RL   Nature 530:331-335(2016).
CC   -!- FUNCTION: Involved in intercistronic processing of primary transcripts
CC       from chloroplast operons. The endonucleolytic activity of the enzyme
CC       depends on the number of phosphates at the 5' end, is inhibited by
CC       structured RNA, and preferentially cleaves A/U-rich sequences.
CC       {ECO:0000256|ARBA:ARBA00023436}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- SIMILARITY: Belongs to the RNase E/G family.
CC       {ECO:0000256|ARBA:ARBA00005522}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KMZ70713.1}.
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DR   EMBL; LFYR01000714; KMZ70713.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0K9PNR8; -.
DR   STRING; 29655.A0A0K9PNR8; -.
DR   OMA; VRDSWMR; -.
DR   OrthoDB; 1206148at2759; -.
DR   Proteomes; UP000036987; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004540; F:RNA nuclease activity; IBA:GO_Central.
DR   GO; GO:2001070; F:starch binding; IEA:InterPro.
DR   GO; GO:0006364; P:rRNA processing; IBA:GO_Central.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   Gene3D; 3.40.1260.20; Ribonuclease E, catalytic domain; 1.
DR   InterPro; IPR013784; Carb-bd-like_fold.
DR   InterPro; IPR002044; CBM_fam20.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR019307; RNA-bd_AU-1/RNase_E/G.
DR   InterPro; IPR004659; RNase_E/G.
DR   NCBIfam; TIGR00757; RNaseEG; 1.
DR   PANTHER; PTHR30001; RIBONUCLEASE; 1.
DR   PANTHER; PTHR30001:SF1; RIBONUCLEASE E_G-LIKE PROTEIN, CHLOROPLASTIC; 1.
DR   Pfam; PF00686; CBM_20; 1.
DR   Pfam; PF10150; RNase_E_G; 1.
DR   SMART; SM01065; CBM_2; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   SUPFAM; SSF49452; Starch-binding domain-like; 1.
DR   PROSITE; PS51166; CBM20; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000036987};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT   DOMAIN          75..185
FT                   /note="CBM20"
FT                   /evidence="ECO:0000259|PROSITE:PS51166"
SQ   SEQUENCE   983 AA;  110334 MW;  E9A9DB032689031E CRC64;
     MELREACRPP LLVFGRNGGS GLRSAGIRRS KISLLPPPHF CYGMPVTKYF KTTQMHIINY
     AHKSAASTLK GCSPIAFLGL CKVMWVIKAD VADGQILYIT GDPVALGCWN PEMAIPLSPS
     NGHAHLWNAE IKVPFGIHFK YNYFIKEKDK PSNDVVWRPG PEFSISIPFP SKQSEYITVS
     DLWLKKRIRN PVNSLWSSWI SDNDHSSKVL KMEDYQTSQT GEHTIQQSDR DHLIGEIFTN
     YSLTDSGNSR VGKNININGI DSRKSFSERD QPVEEPWLFG SIFPCIVDSP IASDASDHPK
     VAEVEVMEQE SNKKQCLVPA TEHQLVRIDE SVSTDILINS SVCTMQRIAI LENGKLVELL
     LEPVKNNVQC DNVYLGVVTK LVPHMGGAFV DIGISRPSLM DIKQNREPFA YPPFKSKIKG
     QKINGSIKIQ SEEHIDTEEH ELDVCVDENI DHDSLEADQH SSIHDVFDDH EVEDTIDISD
     SQESVSSETD YDGSEVELDA EHEENDHPIE SKVTEDTRSS KVKWVHVRKG TKVIVQVVKE
     GLGTKGPSLT ACPNLRSRFW ILISRCNRIG VSKKITGGER TRLRVIAKTL QPPGFGVTVR
     TVAAGHSLEE LQKDLEGLLS TWKGIVEHAK SAALAADDGV EGAVPVILHR AMGQTLSVVQ
     DYFNDKVKNM FVDSPRTYHE VSNYLQEIAP DLCNRVVLYK KSTPIFDDYG LEEEIDNILC
     KRVLLSNGGS LVIEQTEALV SIDVNGGHSM LGQGTSQEKA VLDVNLAAAK QIARELRLRD
     IGGIIVVDFI DMADESNKRL VYEEIKKAVE RDKSMVKVSE LSRHGLMEIT RKRVRPSVSF
     LISEPCICCH GTGRVEALET SFSKIEREIL RMLATWNQKT DPENAKSWPR FVLKVDRYMC
     NYLTSGKRTK LAILSSSLKV WILLKVARGF IRGEFEVKRF MDDKANNEDL VTISRLQSAE
     ERSYIPNSKL TLFPIKKWKS RGR
//