ID A0A0L0BS05_LUCCU Unreviewed; 2379 AA.
AC A0A0L0BS05;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=DNA polymerase zeta catalytic subunit {ECO:0000256|ARBA:ARBA00021589};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=FF38_12064 {ECO:0000313|EMBL:KNC21994.1};
OS Lucilia cuprina (Green bottle fly) (Australian sheep blowfly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Oestroidea;
OC Calliphoridae; Luciliinae; Lucilia.
OX NCBI_TaxID=7375 {ECO:0000313|EMBL:KNC21994.1, ECO:0000313|Proteomes:UP000037069};
RN [1] {ECO:0000313|EMBL:KNC21994.1, ECO:0000313|Proteomes:UP000037069}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LS {ECO:0000313|EMBL:KNC21994.1,
RC ECO:0000313|Proteomes:UP000037069};
RC TISSUE=Full body {ECO:0000313|EMBL:KNC21994.1};
RX PubMed=26108605; DOI=10.1038/ncomms8344;
RA Anstead C.A., Korhonen P.K., Young N.D., Hall R.S., Jex A.R., Murali S.C.,
RA Hughes D.S., Lee S.F., Perry T., Stroehlein A.J., Ansell B.R.,
RA Breugelmans B., Hofmann A., Qu J., Dugan S., Lee S.L., Chao H., Dinh H.,
RA Han Y., Doddapaneni H.V., Worley K.C., Muzny D.M., Ioannidis P.,
RA Waterhouse R.M., Zdobnov E.M., James P.J., Bagnall N.H., Kotze A.C.,
RA Gibbs R.A., Richards S., Batterham P., Gasser R.B.;
RT "Lucilia cuprina genome unlocks parasitic fly biology to underpin future
RT interventions.";
RL Nat. Commun. 6:7344-7344(2015).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KNC21994.1}.
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DR EMBL; JRES01001567; KNC21994.1; -; Genomic_DNA.
DR STRING; 7375.A0A0L0BS05; -.
DR EnsemblMetazoa; KNC21994; KNC21994; FF38_12064.
DR OMA; CYSELRG; -.
DR Proteomes; UP000037069; Unassembled WGS sequence.
DR GO; GO:0016035; C:zeta DNA polymerase complex; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0019985; P:translesion synthesis; IEA:InterPro.
DR CDD; cd05778; DNA_polB_zeta_exo; 1.
DR CDD; cd05534; POLBc_zeta; 1.
DR Gene3D; 1.10.132.60; DNA polymerase family B, C-terminal domain; 1.
DR Gene3D; 3.30.342.10; DNA Polymerase, chain B, domain 1; 1.
DR Gene3D; 1.10.287.690; Helix hairpin bin; 1.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR030559; PolZ_Rev3.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR025687; Znf-C4pol.
DR PANTHER; PTHR45812; DNA POLYMERASE ZETA CATALYTIC SUBUNIT; 1.
DR PANTHER; PTHR45812:SF1; DNA POLYMERASE ZETA CATALYTIC SUBUNIT; 1.
DR Pfam; PF00136; DNA_pol_B; 1.
DR Pfam; PF03104; DNA_pol_B_exo1; 2.
DR Pfam; PF14260; zf-C4pol; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000037069};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 102..189
FT /note="DNA-directed DNA polymerase family B exonuclease"
FT /evidence="ECO:0000259|Pfam:PF03104"
FT DOMAIN 1551..1724
FT /note="DNA-directed DNA polymerase family B exonuclease"
FT /evidence="ECO:0000259|Pfam:PF03104"
FT DOMAIN 1788..2238
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
FT DOMAIN 2290..2356
FT /note="C4-type zinc-finger of DNA polymerase delta"
FT /evidence="ECO:0000259|Pfam:PF14260"
FT REGION 575..614
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 962..981
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1109..1128
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1362..1400
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1446..1482
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 576..600
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1112..1128
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1375..1392
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1446..1460
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1461..1482
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2379 AA; 270444 MW; 218F2DB70D51C92A CRC64;
MSGAEGIFSL RLVIADFYME KPVPGLDACY SEFRGKEIKR VPVIRIFGPN AQGQKTCMHV
HGVFPYFYIP YDQKDFESLN KAIYQIALNL DKAINISLSQ GSSSAQHVFK IQLVKGIPFY
GYHRSDHQFL KIYMFNPRFV RRAANLLQNG AILNKNFHPH ESHVPYILQF MIDYNLYGMS
FLHIPLEIVK FRRCDTAEVV PFRNLHETQM LDVNVVKKIS CSTLEIDIGS NFILNRFQLI
TKTNTTHTNP GIESIWNDER LRRQKLAKSV EDDEEKLNAI PRLEIPATQE RPDIQPTESE
IFYRTALLSK LLAMETSILE TTQISNQTLN SSLVSNVTLN PSNTSLNSKQ QKRTFNLTKI
LQNSVYPEEC TQNDNLINAS VIPNHLNNTS IFEQSLMTKS NETKEQEIIK TAEDESVFLE
DTIVDEELVL SLTQQQNRPP EEEHVPMNVT LREEDMELLD ILQQLEENNE DKIDLDSSLA
PMSQSHKNFP LSPQELDKET TNAALHKPST DTDSEDDQGN DDSIHDFSIA LENLDDLILK
LTQSQKQPLS PNENAALDVP QVDGADDLLR TPTKAAKTAA ANNRSTPPHT PTTPKNRRLT
MGSPCRSPRT PKTGHKYAAL PLVSIDRSTG KTKAVNDTTP LKQSSGERNS LPMLSLRKKL
ALSDMRRKSI SVDTDVEDHV TAVDERTSSG RRRSKRLVNL DKTHVTCALT PRRTNEKLLK
VYSLDNKDME TNVNEEESHN IPQECEVIKD HLKENLKMKE KLQITVANVK ESMENPAKTV
NKRVLKATRK FPEKPDFAVN VANNLEVQFK ETVANNLEAV KEVSSNSETD EEIIDSDETE
EPIKNLRKTP NLKRLRRSFI NANSAKRRKI TITNTSSIVN SSKFDYAIDE CKSNEIVKEN
HLKLKKCKVR LKKLLKSKEQ KDKNTEVVRK AAESNVETLE NANDALKYDM ELKDNTKQVL
KPKVSESVTT KEDNSGSSNL ISNKLEDLSS ALKMHFQTSL TSKVMPETIH SKMESIEMMY
KSPKDDKSKT NNHNIETQNS TIFENLENLQ DALKLLVPLK KNCKNNEVAI ENLTKTPSLD
IFPVSQIKES SDEEISETPL FDILSNTPNK ECSDEEITET PPQETNKDCK VKRLGSSKSI
NLQSPIIFTQ NSSPVLQAQS KTTTRSNSLQ LESYITISSS SNHSSITSET NIVLTPLDLP
PSREEVLKGC RKFQLPEYDF QQPFYSNPDD ITKHKELGFT VLQIPGNKLN DIEEFQSILG
TDVKGLTSWR RQQLIKLGGV EMLNKYRNPQ RIREYLANKK TVIITPQVNA PTRQEAKLWL
KARELLKTKG EEVEDMKMKN LEEDSPIKIR RQKVTMMMNA GDAGDADSDD GDNSLDCSSR
SLTLTPLTPG TPVANEHYTK GELKKSFTPV SRNLEKVPLK LDSSLRKRKN YRIFRRVAPN
KQLKEAFDKE ASKESSQELE TVKDSQGSVI PNSQNSETSV SSETALAELE RSSFMQQLKE
ADVTIGGFSE NSTINNTFGF KVALENLQQA KADVEFNYLT IVTLEVFVAT RDDLFPNPQI
DEIRCLFYAI ENSIPNDHID VDKKLPRKAC GYIMVHDSRD IVAKEGRIHG IDEDIEVTIV
QTEVEALEAL LGLCSRWDPD IYAGYEIEMS SWGFVIERAK YLCLNIAPLL SRVPTQKVRE
FVDEDRESFT DLDVEMKLCG RILLDVWRLL RSEIALTSYT FENVMYHIMH KRCPWHSYRN
LTKWFSSPCS RWIVIEYYLE RVRGTLALLD QLDLIGRTSE MAKLIGIQFY EVLSRGSQFR
VESMMLRIAK PKNLVPLSPS VQQRAHMRAP EYLALIMEPQ SRFYADPLIV LDFQSLYPSM
IIAYNYCFST CLGRVEHLGQ STPFEFGASQ LRVSPAMLRK LVDRDLITIS PCGVVFVKSA
VREGILPRML TEILDTRQMV KQSMKLHKSN SALQRILHSR QLGLKLMANV TYGYTAANFS
GRMPAVEVGD SVVSKGRETL ERAIKMVEAN DKWNVRVVYG DTDSMFVLVP GRNREEAFRI
GEEIAEAVTK QNPHPVKLKL EKVYQPCILQ TKKRYVGYMY ETPDQKEPVY EAKGIETVRR
DGCPMVAKML EKVLRILFET TDVSQVKHYV CRQFTKLLSG RANLQDLIFA KEFRGLNGYR
PTACVPALEL TRKWIQSDPR RIPRRGERVP FIITNGPPGV PLIRLVRHPH EVLSDEGLKI
NAVYYITKAI IPPLNRCLLL IGADVSEWFA NLPRKILLTP ALSTASEIIS CPNPKSKKST
ISQFFSTTNC IVDCGRQTKR GICMECSQNS TRSMIILQSK ISKIERSYLA TQHICQACCG
RVGDLKCGSL DCPVLYVLEV KRRDLQQIMH FRNIVEERF
//
