ID   A0A0L0KRJ7_9ACTN        Unreviewed;       667 AA.
AC   A0A0L0KRJ7;
DT   11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT   11-NOV-2015, sequence version 1.
DT   27-MAR-2024, entry version 26.
DE   RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE            EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN   ORFNames=IQ63_00300 {ECO:0000313|EMBL:KND40335.1};
OS   Streptomyces acidiscabies.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=42234 {ECO:0000313|EMBL:KND40335.1, ECO:0000313|Proteomes:UP000037151};
RN   [1] {ECO:0000313|Proteomes:UP000037151}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCPPB 4445 {ECO:0000313|Proteomes:UP000037151};
RA   Harrison J., Sapp M., Thwaites R., Studholme D.J.;
RT   "Genome sequencing of plant-pathogenic Streptomyces species.";
RL   Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC         N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC         COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC         Evidence={ECO:0000256|ARBA:ARBA00001279};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KND40335.1}.
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DR   EMBL; JPPY01000002; KND40335.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0L0KRJ7; -.
DR   PATRIC; fig|42234.21.peg.66; -.
DR   OrthoDB; 9784823at2; -.
DR   Proteomes; UP000037151; Unassembled WGS sequence.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 3.90.220.20; DNA methylase specificity domains; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR003356; DNA_methylase_A-5.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR044946; Restrct_endonuc_typeI_TRD_sf.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR42998:SF1; TYPE I RESTRICTION ENZYME HINDI METHYLASE SUBUNIT; 1.
DR   PANTHER; PTHR42998; TYPE I RESTRICTION ENZYME HINDVIIP M PROTEIN-RELATED; 1.
DR   Pfam; PF02384; N6_Mtase; 1.
DR   PRINTS; PR00507; N12N6MTFRASE.
DR   SUPFAM; SSF116734; DNA methylase specificity domain; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   4: Predicted;
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   Methyltransferase {ECO:0000313|EMBL:KND40335.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000037151};
KW   Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW   Transferase {ECO:0000313|EMBL:KND40335.1}.
FT   DOMAIN          153..371
FT                   /note="DNA methylase adenine-specific"
FT                   /evidence="ECO:0000259|Pfam:PF02384"
FT   REGION          491..517
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        491..508
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   667 AA;  69902 MW;  5C0ECAFD5F042E72 CRC64;
     MTAAEISRLA GVTRATVSNW RRRHDDFPSP TGGTDSSPLY DWGSVRVWLA MRGHTAAANP
     REEFRTALRL HAQGGVALLP VILVASRHTP EDLTALMDLT DADLATRVTA EAPDTAALEP
     ADGAVLRALL RCVRDEGGRA ALGILAEREL EDSAASGVYR TPAPLADLAA RLLPQEACRV
     LDPACGSGTL LEAAARYGAT ELYGQDVLPV QVQRSTVGLL LEDPDAEITI KSGDSLRSDA
     FPGLMADAVL CNPPYGDRDW GHDELAYDPR WAYGVPARGE SELAWVQHAL AHLAPGGWAV
     LILPPATATR VAGRRIRAEL VRGGAVRAVI ALPAGAAPPL HVGLQIWALQ RPEPGGAERK
     SVLFVDTTEA GAEVALPHWR AFTASPDVFT GESGVARAVP VVDLLDDVVD LTPARRVRVS
     RTDVDPAELA AVVDAGRREL AGAAESLVRG VGLTEWEAKG TSARGWRTAT ASDLARGGAL
     TLLRTVPGTK GQTADSGAVL TSSDIGGGTA PSGDPAEVGV ENAPIVAAGD VLMRSVAGGT
     GPMARVADES DVGALLGPNV HLLRPDPVRL DPWFLVGFLD SADNIAGAST GSTVLHVAPG
     RLRIPLMPLE EQRRYGEAFR RLYVLRADAR RTAQLAEETA GLLAEGLTCG ALLPPSSARD
     SPDVDSS
//