ID A0A0L0MFT9_9BURK Unreviewed; 862 AA.
AC A0A0L0MFT9;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=phosphoenolpyruvate--protein phosphotransferase {ECO:0000256|ARBA:ARBA00012232};
DE EC=2.7.3.9 {ECO:0000256|ARBA:ARBA00012232};
GN ORFNames=BVER_00938c {ECO:0000313|EMBL:KND60839.1};
OS Candidatus Burkholderia verschuerenii.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia.
OX NCBI_TaxID=242163 {ECO:0000313|EMBL:KND60839.1, ECO:0000313|Proteomes:UP000036959};
RN [1] {ECO:0000313|Proteomes:UP000036959}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UZHbot4 {ECO:0000313|Proteomes:UP000036959};
RA Carlier A., Eberl L., Pinto-Carbo M.;
RT "Comparative genomics of Burkholderia leaf nodule symbionts.";
RL Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-
CC L-histidyl-[protein] + pyruvate; Xref=Rhea:RHEA:23880, Rhea:RHEA-
CC COMP:9745, Rhea:RHEA-COMP:9746, ChEBI:CHEBI:15361, ChEBI:CHEBI:29979,
CC ChEBI:CHEBI:58702, ChEBI:CHEBI:64837; EC=2.7.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00000683};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the PEP-utilizing enzyme family.
CC {ECO:0000256|ARBA:ARBA00007837}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KND60839.1}.
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DR EMBL; LFJJ01000042; KND60839.1; -; Genomic_DNA.
DR RefSeq; WP_050453256.1; NZ_LFJJ01000042.1.
DR AlphaFoldDB; A0A0L0MFT9; -.
DR PATRIC; fig|242163.4.peg.5126; -.
DR Proteomes; UP000036959; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:InterPro.
DR Gene3D; 2.70.70.10; Glucose Permease (Domain IIA); 1.
DR Gene3D; 3.30.1340.10; HPr-like; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR Gene3D; 3.50.30.10; Phosphohistidine domain; 1.
DR Gene3D; 1.10.274.10; PtsI, HPr-binding domain; 1.
DR InterPro; IPR011055; Dup_hybrid_motif.
DR InterPro; IPR000032; HPr-like.
DR InterPro; IPR035895; HPr-like_sf.
DR InterPro; IPR008279; PEP-util_enz_mobile_dom.
DR InterPro; IPR000121; PEP_util_C.
DR InterPro; IPR023151; PEP_util_CS.
DR InterPro; IPR036637; Phosphohistidine_dom_sf.
DR InterPro; IPR006318; PTS_EI-like.
DR InterPro; IPR001127; PTS_EIIA_1_perm.
DR InterPro; IPR008731; PTS_EIN.
DR InterPro; IPR001020; PTS_HPr_His_P_site.
DR InterPro; IPR036618; PtsI_HPr-bd_sf.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR NCBIfam; TIGR00830; PTBA; 1.
DR NCBIfam; TIGR01003; PTS_HPr_family; 1.
DR NCBIfam; TIGR01417; PTS_I_fam; 1.
DR PANTHER; PTHR46244; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR PANTHER; PTHR46244:SF3; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR Pfam; PF05524; PEP-utilisers_N; 1.
DR Pfam; PF00391; PEP-utilizers; 1.
DR Pfam; PF02896; PEP-utilizers_C; 1.
DR Pfam; PF00381; PTS-HPr; 1.
DR Pfam; PF00358; PTS_EIIA_1; 1.
DR PRINTS; PR00107; PHOSPHOCPHPR.
DR PRINTS; PR01736; PHPHTRNFRASE.
DR SUPFAM; SSF51261; Duplicated hybrid motif; 1.
DR SUPFAM; SSF47831; Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain; 1.
DR SUPFAM; SSF55594; HPr-like; 1.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF52009; Phosphohistidine domain; 1.
DR PROSITE; PS00742; PEP_ENZYMES_2; 1.
DR PROSITE; PS51093; PTS_EIIA_TYPE_1; 1.
DR PROSITE; PS00371; PTS_EIIA_TYPE_1_HIS; 1.
DR PROSITE; PS51350; PTS_HPR_DOM; 1.
DR PROSITE; PS00369; PTS_HPR_HIS; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Pyruvate {ECO:0000313|EMBL:KND60839.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000036959};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:KND60839.1};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 37..141
FT /note="PTS EIIA type-1"
FT /evidence="ECO:0000259|PROSITE:PS51093"
FT DOMAIN 185..273
FT /note="HPr"
FT /evidence="ECO:0000259|PROSITE:PS51350"
SQ SEQUENCE 862 AA; 90626 MW; ABAEB805643FCD20 CRC64;
MRRAEESLLN HSPDNANDVV LLAPFAGPVV PLADVPDPVF AQGMFGDGIG IDPLDNVLVA
PCDGVVTHLA RTHHAVTLRT PYGAEVLVHI GIDTVELGGE GFAPMVEQGA SVRAGQPLIE
FNADFVAQHA PSLVSVIAIA NGDAFDVIDR AGRGVLKAGA RLLVLRAKDG EKAEVARSGA
MVLEEARRSL TLGHAGGLHA RPAARAREAA RGFDAKVEVR YAGKKAPVES VVGLLGLGAG
EGSTIELVGI GSQAQQAVEA VAAELLRAAH GEVEESPARA KSPAPVAVAR ASGEPLPPNT
LAGVSASPGI AVGTLVRWDD QDIVPPEQAS GPSAAESRAL DKAIAQVDAE LDETVCTASQ
RGAVGEAGIF AVHRVLLEDP TLVDAARDLI SLGKSAGFAW REAIRAQIAL LNNVDDELLA
ERAADLRDIE KRVLRTLGLT NTAARELPAE AVLAAVEFTP SDLSSLDRQR VTALVMSRGG
AKSHAAIIAR QIGIPALVAI GDALHAIPDG TQVVVNASAG RLEHAPTELD VERARTERDR
LAGVREANRK LSKEAASTKD GRHIEVAANI ATLDDAKAAV ENGADAVGLL RTELLFIHRQ
AAPTVDEHAE SYQGIVDALQ ERTAIIRTLD VGADKEVDYL TLPPEENPAL GLRGIRLAQV
RPDLLDDQLR GLLAVKPLGA VRILLPMVTD SGELQRLRVR IDELAREMGR TEPIEVGVMI
EVPSAALLAD QLAKHADFLS IGTNDLTQYT LAMDRCQPDL AAQSDGLHPA VLRLIKAAVQ
GAEKHGKWVG VCGALGGDPV AAPLLVGLGV TELSVDPVSV PGIKARVRNL DYQLCRQRAE
DCLALESAQA VRAASREIWP QD
//
