ID A0A0L9UPZ1_PHAAN Unreviewed; 660 AA.
AC A0A0L9UPZ1;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=beta-fructofuranosidase {ECO:0000256|ARBA:ARBA00012758};
DE EC=3.2.1.26 {ECO:0000256|ARBA:ARBA00012758};
GN ORFNames=LR48_Vigan06g009200 {ECO:0000313|EMBL:KOM44786.1};
OS Phaseolus angularis (Azuki bean) (Vigna angularis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Vigna.
OX NCBI_TaxID=3914 {ECO:0000313|EMBL:KOM44786.1, ECO:0000313|Proteomes:UP000053144};
RN [1] {ECO:0000313|Proteomes:UP000053144}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Jingnong 6 {ECO:0000313|Proteomes:UP000053144};
RX PubMed=26460024; DOI=10.1073/pnas.1420949112;
RA Yang K., Tian Z., Chen C., Luo L., Zhao B., Wang Z., Yu L., Li Y., Sun Y.,
RA Li W., Chen Y., Li Y., Zhang Y., Ai D., Zhao J., Shang C., Ma Y., Wu B.,
RA Wang M., Gao L., Sun D., Zhang P., Guo F., Wang W., Li Y., Wang J.,
RA Varshney R.K., Wang J., Ling H.Q., Wan P.;
RT "Genome sequencing of adzuki bean (Vigna angularis) provides insight into
RT high starch and low fat accumulation and domestication.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:13213-13218(2015).
CC -!- PATHWAY: Glycan biosynthesis; sucrose metabolism.
CC {ECO:0000256|ARBA:ARBA00004914}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004606}; Single-
CC pass type II membrane protein {ECO:0000256|ARBA:ARBA00004606}. Vacuole
CC lumen {ECO:0000256|ARBA:ARBA00004410}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC {ECO:0000256|ARBA:ARBA00009902, ECO:0000256|RuleBase:RU362110}.
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DR EMBL; CM003376; KOM44786.1; -; Genomic_DNA.
DR RefSeq; XP_017426043.1; XM_017570554.1.
DR AlphaFoldDB; A0A0L9UPZ1; -.
DR STRING; 3914.A0A0L9UPZ1; -.
DR EnsemblPlants; KOM44786; KOM44786; LR48_Vigan06g009200.
DR GeneID; 108334657; -.
DR Gramene; KOM44786; KOM44786; LR48_Vigan06g009200.
DR KEGG; var:108334657; -.
DR OMA; ALWEMPD; -.
DR OrthoDB; 315037at2759; -.
DR Proteomes; UP000053144; Chromosome 6.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005775; C:vacuolar lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18624; GH32_Fruct1-like; 1.
DR InterPro; IPR021792; Beta-fructofuranosidase_N.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR018053; Glyco_hydro_32_AS.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR31953:SF1; ACID BETA-FRUCTOFURANOSIDASE 4, VACUOLAR; 1.
DR PANTHER; PTHR31953; BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED; 1.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR Pfam; PF11837; INV_N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU362110};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362110};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000053144};
KW Signal-anchor {ECO:0000256|ARBA:ARBA00022968};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Vacuole {ECO:0000256|ARBA:ARBA00022554};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT TRANSMEM 36..57
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 14..123
FT /note="Beta-fructofuranosidase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF11837"
FT DOMAIN 131..449
FT /note="Glycosyl hydrolase family 32 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00251"
FT DOMAIN 452..643
FT /note="Glycosyl hydrolase family 32 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08244"
SQ SEQUENCE 660 AA; 73159 MW; 3DCF107F2E67C701 CRC64;
MARSLSSHSE LPCSYVPIPD GSYSPAEPPR HVTKKVLLIS SVLLLLLLSL VTFNAYYPHS
SGDGDRASLT PTTPDEFEFS PHTVPSSMLK PFSRGVSSGV SEKSSWMLKS SNGESYPWSN
SMLSWQRTAY HFQPEKNWMN DPNGPMYYKG WYHFFYQYNP KGAVWGTIVW GHAVSRDLIH
WLHLPLAMVA DQWYDQNGVW TGSATILPDG QVIMLYTGST NESVQVQNLA YPADPSDPLL
VDWIKYPSNP VLFPPPGIDT KDFRDPTTAW LTSEGKWRIS IGSKLNKTGI ALVYDTSDFK
TYERVEGLLR AVPGTGMWEC VDFFPVSSEG ENGLDASING ENVKHVVKVS LDDDRHDYYA
VGTYDEKNAK FTPDDLKNDV GIGLRYDYGI FYASKTFYDQ SKGRRVLWSW IGESDSEYAD
VAKGWASIQG IPRTVTLDKK TGSSLLQWPV AEVESLRLGS NEFENLKVKP GSVVPLEVGT
AAQLDIVAEF EIDNKALEMT GQSNKAYECS TSGGAAERGA LGPFGLLVLA DDDLSEYTPT
YFYVVKGNDG QLKTSFCSDQ LRSSLATDVG KKIFGSSVTV LEDEKLSVRI LVDHSVVESF
AQGGRTCVTS RVYPTKAIYG AARLFLFNNA TEVTVTASVK VWQMNSAFIR PFNPQQSNSI
//
