ID A0A0M0J6H2_9EUKA Unreviewed; 708 AA.
AC A0A0M0J6H2;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=S-formylglutathione hydrolase {ECO:0000256|ARBA:ARBA00016774};
DE EC=3.1.2.12 {ECO:0000256|ARBA:ARBA00012479};
GN ORFNames=Ctob_002947 {ECO:0000313|EMBL:KOO22040.1};
OS Chrysochromulina tobinii.
OC Eukaryota; Haptista; Haptophyta; Prymnesiophyceae; Prymnesiales;
OC Chrysochromulinaceae; Chrysochromulina.
OX NCBI_TaxID=1460289 {ECO:0000313|EMBL:KOO22040.1, ECO:0000313|Proteomes:UP000037460};
RN [1] {ECO:0000313|Proteomes:UP000037460}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CCMP291 {ECO:0000313|Proteomes:UP000037460};
RX PubMed=26397803; DOI=10.1371/journal.pgen.1005469;
RA Hovde B.T., Deodato C.R., Hunsperger H.M., Ryken S.A., Yost W., Jha R.K.,
RA Patterson J., Monnat R.J. Jr., Barlow S.B., Starkenburg S.R.,
RA Cattolico R.A.;
RT "Genome Sequence and Transcriptome Analyses of Chrysochromulina tobin:
RT Metabolic Tools for Enhanced Algal Fitness in the Prominent Order
RT Prymnesiales (Haptophyceae).";
RL PLoS Genet. 11:e1005469-e1005469(2015).
CC -!- SIMILARITY: Belongs to the esterase D family.
CC {ECO:0000256|ARBA:ARBA00005622}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KOO22040.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JWZX01003315; KOO22040.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0M0J6H2; -.
DR OrthoDB; 1077476at2759; -.
DR Proteomes; UP000037460; Unassembled WGS sequence.
DR GO; GO:0051903; F:S-(hydroxymethyl)glutathione dehydrogenase activity; IEA:InterPro.
DR GO; GO:0018738; F:S-formylglutathione hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006069; P:ethanol oxidation; IEA:InterPro.
DR GO; GO:0046294; P:formaldehyde catabolic process; IEA:InterPro.
DR CDD; cd08300; alcohol_DH_class_III; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR013149; ADH-like_C.
DR InterPro; IPR013154; ADH-like_N.
DR InterPro; IPR014183; ADH_3.
DR InterPro; IPR000801; Esterase-like.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR014186; S-formylglutathione_hydrol.
DR NCBIfam; TIGR02818; adh_III_F_hyde; 1.
DR NCBIfam; TIGR02821; fghA_ester_D; 1.
DR PANTHER; PTHR43880; ALCOHOL DEHYDROGENASE; 1.
DR PANTHER; PTHR43880:SF12; ALCOHOL DEHYDROGENASE CLASS-3; 1.
DR Pfam; PF08240; ADH_N; 1.
DR Pfam; PF00107; ADH_zinc_N; 1.
DR Pfam; PF00756; Esterase; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR SUPFAM; SSF50129; GroES-like; 2.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000037460}.
FT DOMAIN 363..449
FT /note="Alcohol dehydrogenase-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08240"
FT DOMAIN 535..667
FT /note="Alcohol dehydrogenase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00107"
FT ACT_SITE 168
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR614186-1"
FT ACT_SITE 246
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR614186-1"
FT ACT_SITE 279
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR614186-1"
SQ SEQUENCE 708 AA; 75433 MW; D9CEF0951E68EA7D CRC64;
MLIWHLLPTL ASALVAPGSR IEILKRISVA GGTLTRFSHS SEETKTPMVA SVFIPPGLEY
ANEIPALYWL SGLTCTDENF CQKSGAFAHA ARHRIAVIVP DTSPRGAGVE GEDQDYDLGT
GAGFYVDATA APWSANYRMH SYITKELPAL VEREFKISPK LRAIFGHSMG GHGALTIAFK
DPEGWASVSA FAPICNPTEC DWGKKAFGAY FGAVSDGAAH DASELLRKHG PFPSLGTVLV
DQGTDDEFLG KGQLRPEALE EAAASVSQPL MLRRREGDHS YYTISTYIGE HVAFHADALK
VKAKAMRAAA AAAAPKRVGA SPLLPVVQPE TAGKAITCKA AVALAPKQPL SIETIIVDPP
KAGEVRVRVI ANALCHTDVY TWEGSDPEGL FPCILGHEAG AIVESVGEGV SSVKPGDHVV
PCYTPQCNEP DCIFCRSPKT NLCPRIRGTQ GKGLMPDGTT RFKRADGTPL YHFMGCSTFS
EYTVLAAISC AKVHTAAPLE KMCLLGCGVS TGWGAVWNTC KVENGASVAV FGCGAVGLSV
IQAAKLSGAS RIIAIDLNPQ KFTAARAFGA TDFVNPKDIE GPIQQHIIKM TQWGVDYSFD
CTGNTEVMRA ALECAHRGWG TSCVIGVAAS GKEISTRPFQ LVTGRKWVGT AFGGWKSRSD
VPKLVERYLD GELEVDAYIT HTFKGVGATN DAFEALHGGD CLRAVVVY
//
