ID A0A0M8VIV8_9ACTN Unreviewed; 573 AA.
AC A0A0M8VIV8;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE RecName: Full=Neutral metalloproteinase {ECO:0000256|RuleBase:RU366073};
DE EC=3.4.24.- {ECO:0000256|RuleBase:RU366073};
GN ORFNames=ADK64_17585 {ECO:0000313|EMBL:KOV64267.1};
OS Streptomyces sp. MMG1121.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1415544 {ECO:0000313|EMBL:KOV64267.1, ECO:0000313|Proteomes:UP000037687};
RN [1] {ECO:0000313|Proteomes:UP000037687}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MMG1121 {ECO:0000313|Proteomes:UP000037687};
RA Ju K.-S., Doroghazi J.R., Metcalf W.W.;
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Extracellular zinc metalloprotease.
CC {ECO:0000256|RuleBase:RU366073}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947,
CC ECO:0000256|RuleBase:RU366073};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|RuleBase:RU366073}.
CC -!- SIMILARITY: Belongs to the peptidase M4 family.
CC {ECO:0000256|ARBA:ARBA00009388, ECO:0000256|RuleBase:RU366073}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KOV64267.1}.
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DR EMBL; LGDV01000186; KOV64267.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0M8VIV8; -.
DR STRING; 1415544.ADK64_17585; -.
DR PATRIC; fig|1415544.3.peg.3769; -.
DR Proteomes; UP000037687; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09597; M4_TLP; 1.
DR Gene3D; 3.10.170.10; -; 1.
DR Gene3D; 3.10.450.490; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR InterPro; IPR011096; FTP_domain.
DR InterPro; IPR025711; PepSY.
DR InterPro; IPR023612; Peptidase_M4.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR InterPro; IPR001570; Peptidase_M4_C_domain.
DR InterPro; IPR013856; Peptidase_M4_domain.
DR PANTHER; PTHR33794; BACILLOLYSIN; 1.
DR PANTHER; PTHR33794:SF1; BACILLOLYSIN; 1.
DR Pfam; PF07504; FTP; 1.
DR Pfam; PF03413; PepSY; 1.
DR Pfam; PF01447; Peptidase_M4; 1.
DR Pfam; PF02868; Peptidase_M4_C; 1.
DR PRINTS; PR00730; THERMOLYSIN.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Cell projection {ECO:0000313|EMBL:KOV64267.1};
KW Cilium {ECO:0000313|EMBL:KOV64267.1};
KW Flagellum {ECO:0000313|EMBL:KOV64267.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU366073};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000256|RuleBase:RU366073};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU366073};
KW Reference proteome {ECO:0000313|Proteomes:UP000037687};
KW Secreted {ECO:0000256|RuleBase:RU366073};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366073};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT DOMAIN 49..88
FT /note="FTP"
FT /evidence="ECO:0000259|Pfam:PF07504"
FT DOMAIN 110..169
FT /note="PepSY"
FT /evidence="ECO:0000259|Pfam:PF03413"
FT DOMAIN 225..376
FT /note="Peptidase M4"
FT /evidence="ECO:0000259|Pfam:PF01447"
FT DOMAIN 395..563
FT /note="Peptidase M4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02868"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 370
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
FT ACT_SITE 466
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
SQ SEQUENCE 573 AA; 60129 MW; B572ACF0DA26E197 CRC64;
MAGTAFAGPD TGVERSAASA DTNATTVVTA ARTAAFAHAS ATGVSQGDEL HAQDVMIDPE
GARHVRFVRT HNSLPVLGGD LVVHLDRKLA YTGVTRAADH AVEPAGRARL TADQAAAKAA
QVAKGDAGTA QLVVDARGGS SALAYQVAVT DEQGTSTVVV DAVTGKVRSN TPDSDEFLSP
KVLDNLRKHG ETADPATGTA AADPAAGLLG SGVSGVTKYP SAAKGTGKTL YVGSVGLTTT
ATSKGHYQLK DPSRYGTETR DAKGSTTEKF SAGAKFTSTT DVWGNGATSS RASAAADAQY
GITKTLDFYK NTFGRKGIAN NSKAAQGMVH WGSKVANAFW DPTCNCMLYG DGDGKTFKKP
LVVLDVTGHE LTHGVVDATA KLEPTYVDPN GNQYGEPGAL NESLADIFGS NVEFYANNKK
DTPDYLIGEK LGLAQKFLRR LDHPSLDKLE GTIDYWSPDT YYTEVHAGSG VSSHAYYLLA
EGSGKKTING VAYDSPTYNH STVKGIGRTK ATAIFYRALT RYMVSTTDFH DARKATLQAA
KDLYGANSTE YKTVDKAWAA VDVTVANTPA ARH
//
