ID A0A0N0CSL9_9LACO Unreviewed; 465 AA.
AC A0A0N0CSL9;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE RecName: Full=Argininosuccinate lyase {ECO:0000256|ARBA:ARBA00012338, ECO:0000256|HAMAP-Rule:MF_00006};
DE Short=ASAL {ECO:0000256|HAMAP-Rule:MF_00006};
DE EC=4.3.2.1 {ECO:0000256|ARBA:ARBA00012338, ECO:0000256|HAMAP-Rule:MF_00006};
DE AltName: Full=Arginosuccinase {ECO:0000256|HAMAP-Rule:MF_00006};
GN Name=argH {ECO:0000256|HAMAP-Rule:MF_00006,
GN ECO:0000313|EMBL:KOY76015.1};
GN ORFNames=RZ71_04490 {ECO:0000313|EMBL:KOY76015.1};
OS Apilactobacillus kunkeei.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Apilactobacillus.
OX NCBI_TaxID=148814 {ECO:0000313|EMBL:KOY76015.1, ECO:0000313|Proteomes:UP000037778};
RN [1] {ECO:0000313|EMBL:KOY76015.1, ECO:0000313|Proteomes:UP000037778}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LAko {ECO:0000313|EMBL:KOY76015.1,
RC ECO:0000313|Proteomes:UP000037778};
RX PubMed=25953738; DOI=10.1093/gbe/evv079;
RA Tamarit D., Ellegaard K.M., Wikander J., Olofsson T., Vasquez A.,
RA Andersson S.G.;
RT "Functionally Structured Genomes in Lactobacillus kunkeei Colonizing the
RT Honey Crop and Food Products of Honeybees and Stingless Bees.";
RL Genome Biol. Evol. 7:1455-1473(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(N(omega)-L-arginino)succinate = fumarate + L-arginine;
CC Xref=Rhea:RHEA:24020, ChEBI:CHEBI:29806, ChEBI:CHEBI:32682,
CC ChEBI:CHEBI:57472; EC=4.3.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000985, ECO:0000256|HAMAP-
CC Rule:MF_00006};
CC -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine
CC from L-ornithine and carbamoyl phosphate: step 3/3.
CC {ECO:0000256|ARBA:ARBA00004941, ECO:0000256|HAMAP-Rule:MF_00006}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00006}.
CC -!- SIMILARITY: Belongs to the lyase 1 family. Argininosuccinate lyase
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_00006}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KOY76015.1}.
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DR EMBL; JXCY01000007; KOY76015.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0N0CSL9; -.
DR PATRIC; fig|148814.8.peg.1375; -.
DR UniPathway; UPA00068; UER00114.
DR Proteomes; UP000037778; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004056; F:argininosuccinate lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0042450; P:arginine biosynthetic process via ornithine; IEA:InterPro.
DR CDD; cd01359; Argininosuccinate_lyase; 1.
DR Gene3D; 1.10.40.30; Fumarase/aspartase (C-terminal domain); 1.
DR Gene3D; 1.20.200.10; Fumarase/aspartase (Central domain); 1.
DR Gene3D; 1.10.275.10; Fumarase/aspartase (N-terminal domain); 1.
DR HAMAP; MF_00006; Arg_succ_lyase; 1.
DR InterPro; IPR029419; Arg_succ_lyase_C.
DR InterPro; IPR009049; Argininosuccinate_lyase.
DR InterPro; IPR024083; Fumarase/histidase_N.
DR InterPro; IPR020557; Fumarate_lyase_CS.
DR InterPro; IPR000362; Fumarate_lyase_fam.
DR InterPro; IPR022761; Fumarate_lyase_N.
DR InterPro; IPR008948; L-Aspartase-like.
DR NCBIfam; TIGR00838; argH; 1.
DR PANTHER; PTHR43814; ARGININOSUCCINATE LYASE; 1.
DR PANTHER; PTHR43814:SF1; ARGININOSUCCINATE LYASE; 1.
DR Pfam; PF14698; ASL_C2; 1.
DR Pfam; PF00206; Lyase_1; 1.
DR PRINTS; PR00145; ARGSUCLYASE.
DR PRINTS; PR00149; FUMRATELYASE.
DR SUPFAM; SSF48557; L-aspartase-like; 1.
DR PROSITE; PS00163; FUMARATE_LYASES; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis {ECO:0000256|HAMAP-Rule:MF_00006};
KW Arginine biosynthesis {ECO:0000256|ARBA:ARBA00022571, ECO:0000256|HAMAP-
KW Rule:MF_00006}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00006};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00006};
KW Reference proteome {ECO:0000313|Proteomes:UP000037778}.
FT DOMAIN 8..302
FT /note="Fumarate lyase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00206"
FT DOMAIN 365..432
FT /note="Argininosuccinate lyase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF14698"
SQ SEQUENCE 465 AA; 52816 MW; FC1E11F8E64BA84B CRC64;
MSEKLWGGRF NGDANDLFLR LGASINVDIK MANEDIEGSI AHATMLSHVG IITEAEKDQI
VNGLKEIKEE FNEGKLHFTY QNEDIHMNVE TVLTEKIGAV AGKLHTARSR NDQVALDFHL
YLKKRLPQIL ELIRSFQSEL VNLADENLET IMPGYTHLQH AQPISFAHYL MAYYEMLDRD
YDRFEFNQKH TNMSPIGAAA LAGTTFPIDP HFTANELGLD APYNNSIDAV SDRDFAIEFL
SNASILMMHL SRLCEEICLW SSYEFKYVEL SDAFSTGSSI MPQKKNPDMC ELIRGKTGVV
YGHLNALLAT MKSLPLAYNK DLQEDKAGVF DTVENIEPIL QLMTEMIATM QVNKDRMLQA
VEKDFSNATE LADYLSAKGL PFRQAHRIAG ELVAKCINEN CYLQDLSLEQ LHNYSDLFEE
DIYEDLKPHV AIQRRKSYGS TGFNEVHKQI EKAKEKLKGE MNHEE
//
