ID   A0A0N0MWS0_9ACTN        Unreviewed;       859 AA.
AC   A0A0N0MWS0;
DT   09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT   09-DEC-2015, sequence version 1.
DT   24-JAN-2024, entry version 25.
DE   SubName: Full=Polynucleotide kinase-phosphatase, bacterial {ECO:0000313|EMBL:KPI10528.1};
DE            EC=3.6.1.17 {ECO:0000313|EMBL:KPI10528.1};
GN   ORFNames=OK074_0163 {ECO:0000313|EMBL:KPI10528.1};
OS   Actinobacteria bacterium OK074.
OC   Bacteria; Actinomycetota; Actinomycetes.
OX   NCBI_TaxID=1592327 {ECO:0000313|EMBL:KPI10528.1, ECO:0000313|Proteomes:UP000037991};
RN   [1] {ECO:0000313|EMBL:KPI10528.1, ECO:0000313|Proteomes:UP000037991}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OK074 {ECO:0000313|EMBL:KPI10528.1,
RC   ECO:0000313|Proteomes:UP000037991};
RA   Brown S.D., Utturkar S.M., Klingeman D.M., Pelletier D.;
RT   "Draft genome sequences for four actinobacteria strains OK006 OK074 OV450
RT   and OV320.";
RL   Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KPI10528.1}.
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DR   EMBL; LJCV01000221; KPI10528.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0N0MWS0; -.
DR   PATRIC; fig|1592327.3.peg.3527; -.
DR   OrthoDB; 9807890at2; -.
DR   Proteomes; UP000037991; Unassembled WGS sequence.
DR   GO; GO:0004081; F:bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity; IEA:UniProtKB-EC.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   CDD; cd07423; MPP_Prp_like; 1.
DR   Gene3D; 3.60.21.10; -; 1.
DR   Gene3D; 3.30.470.30; DNA ligase/mRNA capping enzyme; 2.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR   InterPro; IPR029052; Metallo-depent_PP-like.
DR   InterPro; IPR041780; MPP_PrpE-like.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR024028; PNKP_bac.
DR   InterPro; IPR032380; PNKP_ligase_dom.
DR   NCBIfam; TIGR04075; bacter_Pnkp; 1.
DR   PANTHER; PTHR12435:SF2; PROTEIN KTI12 HOMOLOG; 1.
DR   PANTHER; PTHR12435; UNCHARACTERIZED; 1.
DR   Pfam; PF13671; AAA_33; 1.
DR   Pfam; PF00149; Metallophos; 1.
DR   Pfam; PF16542; PNKP_ligase; 1.
DR   SUPFAM; SSF56091; DNA ligase/mRNA capping enzyme, catalytic domain; 1.
DR   SUPFAM; SSF56300; Metallo-dependent phosphatases; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE   4: Predicted;
KW   Hydrolase {ECO:0000313|EMBL:KPI10528.1};
KW   Kinase {ECO:0000313|EMBL:KPI10528.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000037991};
KW   Transferase {ECO:0000313|EMBL:KPI10528.1}.
FT   DOMAIN          202..388
FT                   /note="Calcineurin-like phosphoesterase"
FT                   /evidence="ECO:0000259|Pfam:PF00149"
FT   DOMAIN          476..854
FT                   /note="Polynucleotide kinase-phosphatase ligase"
FT                   /evidence="ECO:0000259|Pfam:PF16542"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..19
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   859 AA;  94443 MW;  91182CED79652851 CRC64;
     MTENETETQA QAQTQPRHKK RTLPVTDLSL VVLIGASGSG KSTFARRHFK PTEVISSDFC
     RGLVADDEND QSASGDAFDV LHYIAGKRLA AGRRTVVDAT SVQSDSRRQL VELARQYDVL
     PIAIVLDVSE EVCAERNAAR ADRAAMPRRV ITRHTRELRR SLRHLEREGF RKVHVLRGVE
     EVETASVVTE RRFNDLTHLT GPFDIVGDVH GCAAELETLL GQLGYADGVH PDGRTAVFVG
     DLVDRGPDSP GVLRRVMSMV ASGNALCVSG NHENKFGRYL KGRQVQHTHG LAQTIAQMAE
     ESDEFRSLVR EFVDGLISHY VLDGGRLVVC HAGLPEKYHG RTSGRVRSHA LYGDTTGETD
     EFGLPVRYPW AEDYRGRAAV VYGHTPVPEA TWLNNTICLD TGAVFGGRLT ALRWPEREVV
     DVPAERVWYE PARPLRTEAP GGADGRPLDL ADVHGRRVVE TRYAGRVTVR EENAAAALEV
     MSRFAVDPRL LPYLPPTMAP TPTSQVEGYL EHPAEAFAQY AADGVARVVC EEKHMGSRAV
     ALVCRDAEAA RRRFGVDGPT GALYTRTGRP FLSDAAVTEE LLDRVRKSAD AAGLWDELAT
     DWLLLDAELM PWSLKASGLL RAQYAAVGAA SGAVFPGALA ALDRAAARGT DVRDLLARQR
     ERAADAAAFT DAYRRYCWPT DGLEGVRLAP FQLLAVQGRS LAALPHAEQL ALLDRLVEHD
     GSGLLQTTRR LYVDTGDAES IGAGTDWWLE LTARGGEGMV VKPLDALVRD GRGRLVQPGV
     KCRGREYLRI IYGPEYTRPD NLARLRHRSL NHKRSLAIRE YALGTEALDR LAEGEPLWRV
     HEAVFAVLAL ESEPVDPRL
//