ID   A0A0N0P530_LEPSE        Unreviewed;       972 AA.
AC   A0A0N0P530;
DT   09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT   09-DEC-2015, sequence version 1.
DT   27-MAR-2024, entry version 27.
DE   RecName: Full=sphingosine kinase {ECO:0000256|ARBA:ARBA00044037};
DE            EC=2.7.1.91 {ECO:0000256|ARBA:ARBA00044037};
GN   ORFNames=ABL78_4948 {ECO:0000313|EMBL:KPI85986.1};
OS   Leptomonas seymouri.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Leishmaniinae; Leptomonas.
OX   NCBI_TaxID=5684 {ECO:0000313|EMBL:KPI85986.1, ECO:0000313|Proteomes:UP000038009};
RN   [1] {ECO:0000313|EMBL:KPI85986.1, ECO:0000313|Proteomes:UP000038009}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 30220 {ECO:0000313|EMBL:KPI85986.1,
RC   ECO:0000313|Proteomes:UP000038009};
RX   PubMed=26317207;
RA   Kraeva N., Butenko A., Hlavacova J., Kostygov A., Myskova J., Grybchuk D.,
RA   Lestinova T., Votypka J., Volf P., Opperdoes F., Flegontov P., Lukes J.,
RA   Yurchenko V.;
RT   "Leptomonas seymouri: Adaptations to the Dixenous Life Cycle Analyzed by
RT   Genome Sequencing, Transcriptome Profiling and Co-infection with Leishmania
RT   donovani.";
RL   PLoS Pathog. 11:E1005127-E1005127(2015).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a sphingoid base + ATP = a sphingoid 1-phosphate + ADP + H(+);
CC         Xref=Rhea:RHEA:51496, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:76941, ChEBI:CHEBI:84410, ChEBI:CHEBI:456216;
CC         EC=2.7.1.91; Evidence={ECO:0000256|ARBA:ARBA00043822};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KPI85986.1}.
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DR   EMBL; LJSK01000155; KPI85986.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0N0P530; -.
DR   EnsemblProtists; KPI85986; KPI85986; ABL78_4948.
DR   VEuPathDB; TriTrypDB:Lsey_0155_0070; -.
DR   OMA; HDCEDYV; -.
DR   OrthoDB; 374620at2759; -.
DR   Proteomes; UP000038009; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0017050; F:D-erythro-sphingosine kinase activity; IEA:UniProt.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.200.40; -; 1.
DR   InterPro; IPR017438; ATP-NAD_kinase_N.
DR   InterPro; IPR001206; Diacylglycerol_kinase_cat_dom.
DR   InterPro; IPR016064; NAD/diacylglycerol_kinase_sf.
DR   InterPro; IPR045540; YegS/DAGK_C.
DR   PANTHER; PTHR12358:SF31; LD11247P-RELATED; 1.
DR   PANTHER; PTHR12358; SPHINGOSINE KINASE; 1.
DR   Pfam; PF00781; DAGK_cat; 1.
DR   Pfam; PF19279; YegS_C; 1.
DR   SUPFAM; SSF111331; NAD kinase/diacylglycerol kinase-like; 2.
DR   PROSITE; PS50146; DAGK; 1.
PE   4: Predicted;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:KPI85986.1};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          343..427
FT                   /note="DAGKc"
FT                   /evidence="ECO:0000259|PROSITE:PS50146"
FT   REGION          1..65
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          86..148
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          475..497
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          636..666
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..15
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        16..34
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        641..666
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   972 AA;  105964 MW;  8D6ED292E977E524 CRC64;
     MPSQSAVATS SSLHKPPTAH RRRRQVPTAR PKPKSKLPKK PDGVPAVPTT AEPDSSTAAD
     IASPPSLDCV ASTHVDPTMP VCSIDAGADA TRPSVQSGSR AGVKDSMRAD SSKFSPLRLM
     QPGNESYGPA TPPPHRDPCR AGPAGGRTSA DMTAVVHAKG HTCKLTYSPT KDSLHIARTS
     KSGNIRTVLN IPVHMIINIE TAAEKEDRQR ARQTNDEAVK LVFADSSGGA GLLCTHPTGT
     TTCDAAPLVF AHSRVNTDIS ASGSCSYGGV LFQQNALTSP LGVCNWVTSP LATPATQIRC
     YVHYVRQRNR ERLSIHTLEL LSHGPAEHMQ TVVNALVQRI YRKGPKHILI FISPKSGKGK
     GEQSFDKHVR PLLHFSRHTY QTYITQRAHD CEDYVADLSH PMNANTVVAA VGGDGMVNEV
     VNGLHRRKLA YVQWLRSITV DVLPCNLTED SSQRLSDGGS SNAWGLDPQA LATMSDGTSS
     SAFPKAASSP DPQHANPTIA ATMTAAGALN RQPELEENKR CRCASPDYAA AVEAWRFAQR
     LVEEGWDALM PLIATVPTGS ACGMAKSLDV LSIAQSAMAL VHLTTVHMDL LHMCFTPNKE
     LKECQKKKLS LRKLTAAKED FSKYCKNYAA ELHERAEAET TAHQQQQSQE GEGREEGSSS
     PISALSAADT KSPFLKDGSN VYTDEVNYAL KMPYFANRVA FMSLSFGTAN DIDHGSEPLR
     WMGNTRFHVY GGYLILLGLR CYRGVLRYLP WRSKSGKTME KLHSRHTMPS TDDFPLCTMR
     DDCPHCQDYT FTHCGQFSLA SSNVTEEKHT VNTPRSGRER AGARCRSSAA ATLAPYTDAE
     LLDEDSVDFS DPNMPWVTIR GEFCTALICN VRDIAQDMLM APLSHLSDGA IDVVYCRIDP
     CTGKGGRIEM VKFFMALESG KHVDLDFVNY VKARAVEMKV DEGILMSDGE LMPLSSVRIT
     KLRSSIQVVR NE
//