ID A0A0N1ADT2_9PROT Unreviewed; 943 AA.
AC A0A0N1ADT2;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN ORFNames=IP80_06560 {ECO:0000313|EMBL:KPF49772.1};
OS beta proteobacterium AAP65.
OC Bacteria; Pseudomonadota; Betaproteobacteria.
OX NCBI_TaxID=1523424 {ECO:0000313|EMBL:KPF49772.1, ECO:0000313|Proteomes:UP000037849};
RN [1] {ECO:0000313|EMBL:KPF49772.1, ECO:0000313|Proteomes:UP000037849}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AAP65 {ECO:0000313|EMBL:KPF49772.1,
RC ECO:0000313|Proteomes:UP000037849};
RA Zeng Y., Feng F., Liu Y., Koblizek M.;
RT "Novel Diversity of Limnic Aerobic Anoxygenic Phototrophic Bacteria as
RT Revealed by High-throughput Strain Identification and Genome Sequencing.";
RL Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPF49772.1}.
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DR EMBL; LJHW01000006; KPF49772.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0N1ADT2; -.
DR STRING; 1523424.IP80_06560; -.
DR PATRIC; fig|1523424.3.peg.3834; -.
DR OrthoDB; 9800174at2; -.
DR Proteomes; UP000037849; Unassembled WGS sequence.
DR GO; GO:0051060; F:pullulanase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR Gene3D; 2.60.40.1130; Rab geranylgeranyltransferase alpha-subunit, insert domain; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR011839; Pullul_strch.
DR InterPro; IPR024561; Pullul_strch_C.
DR InterPro; IPR040671; Pullulanase_N2.
DR NCBIfam; TIGR02103; pullul_strch; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF11852; Pullul_strch_C; 1.
DR Pfam; PF17967; Pullulanase_N2; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 2.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000037849};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..943
FT /note="pullulanase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5005865577"
FT DOMAIN 414..789
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 943 AA; 101958 MW; C806B9205A6010FF CRC64;
MHPAHRLSAV FTLGVWACAA TAQAAAPTTA ACAAGFETVL QAAPAAAAPL PAQAVWLNRQ
WLRWPGLNAA PAGGGYRLHF SATGQINARP GAPVAGADGA LTLDLRSAAL PAALHQRWRW
VGQGATLAVR AADQHRLREL LRGQLVLTLE DAEGRVLQAT RAQTPGALDD LYARAEQLPD
LGATITRTRV RGGGDADGWR TGFRLWAPTA QAVHLCLHPG ADAPASAVHT LQRDKATGAW
GLQLPGDLSQ AQATHGGHYY TYLVDVALPG GGVVRNRVTD PYSLSLNANS QRSWIGRLDD
PRWLPEGWAQ TKRPATVRHN TDLVIYELHL RDFSVRDTTV PAAHRGSYLA FTHPQSAGMQ
HLRTLAAAGL TDVHLLPVFD LATVPEQGCT SPDEAHLAAL PPDSEEQQAL VAATTAQDCF
NWGYDPLHFT APEGSYATEA NDGGARILEF RRMVMALHRA GLRVGMDMVY NHTSASGQTP
KSVLDRIVPG YYHRLNAQGG VETSTCCDNT ATEHRMMAKL MIDSAVVWAR DHRIDSMRFD
LMGHQPRAAM ERLQRAVNRA AGQTIHLLGE GWNFGEVKDG ARFVQAAQKS LNGSGIATFS
DRGRDAARGG GCCDGPAEVL KHQGWLNGLH YAPNPQAQAA GAGTRAELLR SADLIRAGLA
GTLQGYRLQT ADGSVKRLDQ IDYAGQGAGY ASQPGEVVNY VENHDNPTLW DINLLKLPPD
TPREDRARVQ VLGGALTAFS QGVAYLHAGV ELLRSKNLDR NSYDSGDWFN RIDWTLQDNY
FGGGLPPKRE GEGLWPAARP LLARAAEIKP TPAEMRYSRD AFLDLLRIRA SSTLFRLRTA
EEVQRRLTMF NTGPDQVATV AVGHLNGRGL RGARFAEVLY AINVAPEAAE LRLPTLRGRP
LVLHPVHRGP GAADPRPAAQ AQWDPATATL RVPPRTALVY VRE
//
