ID   A0A0N7J9B0_9BURK        Unreviewed;       898 AA.
AC   A0A0N7J9B0;
DT   20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT   20-JAN-2016, sequence version 1.
DT   27-MAR-2024, entry version 24.
DE   SubName: Full=Pullulanase {ECO:0000313|EMBL:ALK90488.1};
DE            EC=3.2.1.41 {ECO:0000313|EMBL:ALK90488.1};
GN   Name=pulA {ECO:0000313|EMBL:ALK90488.1};
GN   ORFNames=L103DPR2_00074 {ECO:0000313|EMBL:ALK90488.1};
OS   Limnohabitans sp. 103DPR2.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Limnohabitans.
OX   NCBI_TaxID=1678129 {ECO:0000313|EMBL:ALK90488.1, ECO:0000313|Proteomes:UP000063254};
RN   [1] {ECO:0000313|EMBL:ALK90488.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=103DPR2 {ECO:0000313|EMBL:ALK90488.1};
RA   Ahn J.-H., Kim S.B.;
RT   "Limnohabitans sp. 2 strains.";
RL   Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; CP011834; ALK90488.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0N7J9B0; -.
DR   STRING; 1678129.L103DPR2_00074; -.
DR   KEGG; lim:L103DPR2_00074; -.
DR   PATRIC; fig|1678129.3.peg.65; -.
DR   OrthoDB; 9800174at2; -.
DR   Proteomes; UP000063254; Chromosome.
DR   GO; GO:0051060; F:pullulanase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR   CDD; cd02860; E_set_Pullulanase; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   Gene3D; 2.60.40.1130; Rab geranylgeranyltransferase alpha-subunit, insert domain; 1.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR024561; Pullul_strch_C.
DR   InterPro; IPR040671; Pullulanase_N2.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   Pfam; PF11852; Pullul_strch_C; 1.
DR   Pfam; PF17967; Pullulanase_N2; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 2.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   4: Predicted;
KW   Glycosidase {ECO:0000313|EMBL:ALK90488.1};
KW   Hydrolase {ECO:0000313|EMBL:ALK90488.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000063254};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           29..898
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5006013932"
FT   DOMAIN          58..161
FT                   /note="Pullulanase N2"
FT                   /evidence="ECO:0000259|Pfam:PF17967"
FT   DOMAIN          173..264
FT                   /note="Glycoside hydrolase family 13 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF02922"
FT   DOMAIN          730..892
FT                   /note="Alpha-1,6-glucosidases pullulanase-type C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF11852"
SQ   SEQUENCE   898 AA;  98138 MW;  99776BACB445BAD2 CRC64;
     MPSFASAPAW RAPLCSIPAM LALTSSMAAD IPRDANLSMC DAPLGHQTLQ ANDLGLPAAA
     IWLNATHIQW PQGAGRGTPA SALPWRLLAS RKGTLQIQVG QQAIGHDQSW PLQGNDDVRE
     LSGLSTDELR RLHASQLVLV QEDSEGRVQH ATRIQAAKAL DTLFAPASLN ASLGATITQQ
     PHKHAADQTT FAIWAPTALK VSVCLYPKAR GKANGKLDLL RDDVSGVWSS TTQKAPQGSY
     YRYAVEVFVP QVGRVINHVN DPYAVSLSAN SQRSYVADLN AAHLKPANWD THTSPSSVKH
     AVDMRIYELH VRDFSIQDKT VPVSMRGKYL ALTQKQSQGM RHLKALAQSG MTDLHLLPVF
     DFATVPEKNC QSSLNAKVAL TPTMRTQDCF NWGYDPLHFN APEGSYASDA EDGAKRILEF
     RQMVKSLNEV GLRVGMDMVY NHTSASGQHL HSVLDRIVPG YYQRLNAQGE VERSTCCDNT
     ATEHLMMGKL MSDSVLMWAA HYKLDSFRFD LMGHQPRQVM VDMQERVQQV TGRRIDFVGE
     GWNFGEVANG SRFVQASQLS LNGTGIGTFN DRLRDAIRGG GASDAVETLV KMPGFVSGQE
     KSAHVAAQIR AGLAGSLRTY RMVTADGNVK ALQDLPYGDQ PTGYASEPSE VVNYAENHDN
     LTLFDSLAYK LPLDTPSSER ARVQHLASAL TAFSQGIAYF HAGMDILRSK SLDGNSYDSG
     DAFNVLDWSY KHNGFGRAVP ELNGTASAKA LSTSLLQNQK LQPTPQDIAW TRDAFRDVLK
     IRQSTELLRL KSAQAIQDRL TFLNTEAQAD PRLIVGHVDG RGLKDFAALI YFVNVAPEAK
     HFSAPTLAHQ AFELHPVLAD AKAADERIRH AARYTASTGG FDIPARSAVV FIVKGGQP
//