ID A0A0Q5NA61_9MICO Unreviewed; 685 AA.
AC A0A0Q5NA61;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN ORFNames=ASF89_02425 {ECO:0000313|EMBL:KQR66036.1};
OS Frigoribacterium sp. Leaf172.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Frigoribacterium.
OX NCBI_TaxID=1736285 {ECO:0000313|EMBL:KQR66036.1, ECO:0000313|Proteomes:UP000051720};
RN [1] {ECO:0000313|EMBL:KQR66036.1, ECO:0000313|Proteomes:UP000051720}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf172 {ECO:0000313|EMBL:KQR66036.1,
RC ECO:0000313|Proteomes:UP000051720};
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KQR66036.1, ECO:0000313|Proteomes:UP000051720}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf172 {ECO:0000313|EMBL:KQR66036.1,
RC ECO:0000313|Proteomes:UP000051720};
RA Schulze-Lefert P.;
RT "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KQR66036.1}.
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DR EMBL; LMPB01000001; KQR66036.1; -; Genomic_DNA.
DR RefSeq; WP_055811805.1; NZ_LMPB01000001.1.
DR AlphaFoldDB; A0A0Q5NA61; -.
DR STRING; 1736285.ASF89_02425; -.
DR OrthoDB; 9800974at2; -.
DR Proteomes; UP000051720; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF1; BETA-GALACTOSIDASE GANA; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084};
KW Reference proteome {ECO:0000313|Proteomes:UP000051720}.
FT DOMAIN 15..380
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 401..604
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT ACT_SITE 151
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 305
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 112
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 150
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 685 AA; 74526 MW; 0AFFDB4B930C8407 CRC64;
MSVVRGVDRV LFGGDYNPEQ WPEHVWHDDA RLMAIAGVNT VTLGVFSWSS IEPREGEYDF
GWLDRVIDLM HAHGIGVVLA TPTASPPPWF TKAHPEALNR TADGVQLVHG SRDVYDPSAD
AYREAARRVA TALGERYGRH EAVVAWHVHN EYGSVSYGPA TDRRFREWLR ARYGDLGRLN
EAWWSAFWSQ RYAEWDEILA PQATQYLPNP TQMLDFRRFS AEVLLECYRE QAEILRALSP
DVPLTTNFML PTWLHFDHWD FGAEVDFVSI DHYSDSPGVE GAAHAAFGAD QARSFARGRP
WLLMEQGANV TQVPGRMLVK EPGEVIRTSL QHVARGSDGA LFFQWRAPLA GAEVFHSSMV
PHSGPDSRAF REMTELGRVL GRLTDVVAPP EGSDARVVTP RVAIAWEPDS WWALESASLP
SDHFSLLASV RRAHRELWFA GVSADLVRLD DDPAALDAYD LLLVPSQLLA TDEQAAGLDA
WVRRGGHAVV WCFSGTVDAD LHVRPGGYSG AFAETLGIRV EEPHPLPKGV AIAIEGGGRA
HDWTEHVELR GASVVTRYAE GVLSGEPAIT ENRRGDGVAT YVSAELDDAT FTRVVTSALA
AAGVRAEAPG AGGGVEVVRR HAGSGVVVAA INHTAETRTV TLRGTPIDLE GGADGADGAD
GPVVDGPVDL APGAFVFLHE APSPS
//
