ID A0A0Q8LI65_9MICO Unreviewed; 455 AA.
AC A0A0Q8LI65;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=Small RNA 2'-O-methyltransferase {ECO:0000256|ARBA:ARBA00021330};
DE EC=2.1.1.386 {ECO:0000256|ARBA:ARBA00035025};
GN ORFNames=ASD93_11125 {ECO:0000313|EMBL:KRB36598.1};
OS Microbacterium sp. Root180.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microbacterium.
OX NCBI_TaxID=1736483 {ECO:0000313|EMBL:KRB36598.1, ECO:0000313|Proteomes:UP000050802};
RN [1] {ECO:0000313|EMBL:KRB36598.1, ECO:0000313|Proteomes:UP000050802}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Root180 {ECO:0000313|EMBL:KRB36598.1,
RC ECO:0000313|Proteomes:UP000050802};
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KRB36598.1, ECO:0000313|Proteomes:UP000050802}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Root180 {ECO:0000313|EMBL:KRB36598.1,
RC ECO:0000313|Proteomes:UP000050802};
RA Schulze-Lefert P.;
RT "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + small RNA 3'-end nucleotide = H(+) +
CC S-adenosyl-L-homocysteine + small RNA 3'-end 2'-O-methylnucleotide;
CC Xref=Rhea:RHEA:37887, Rhea:RHEA-COMP:10415, Rhea:RHEA-COMP:10416,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74896, ChEBI:CHEBI:74898; EC=2.1.1.386;
CC Evidence={ECO:0000256|ARBA:ARBA00034992};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. HEN1 family.
CC {ECO:0000256|ARBA:ARBA00009026}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRB36598.1}.
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DR EMBL; LMHS01000003; KRB36598.1; -; Genomic_DNA.
DR RefSeq; WP_056122294.1; NZ_LMHS01000003.1.
DR AlphaFoldDB; A0A0Q8LI65; -.
DR STRING; 1736483.ASD93_11125; -.
DR OrthoDB; 626362at2; -.
DR Proteomes; UP000050802; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008171; F:O-methyltransferase activity; IEA:InterPro.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0031047; P:regulatory ncRNA-mediated gene silencing; IEA:UniProtKB-KW.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.30.1610.20; Hen1, N-terminal domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR024026; 3'-RNA_MeTfrase_Hen1_bac.
DR InterPro; IPR026610; Hen1.
DR InterPro; IPR024740; Hen1_N.
DR InterPro; IPR038546; Hen1_N_sf.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR NCBIfam; TIGR04074; bacter_Hen1; 1.
DR PANTHER; PTHR21404; HEN1; 1.
DR PANTHER; PTHR21404:SF3; SMALL RNA 2'-O-METHYLTRANSFERASE; 1.
DR Pfam; PF12623; Hen1_L; 1.
DR Pfam; PF13489; Methyltransf_23; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:KRB36598.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000050802};
KW RNA-mediated gene silencing {ECO:0000256|ARBA:ARBA00023158};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:KRB36598.1}.
FT DOMAIN 1..245
FT /note="Hen1 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12623"
SQ SEQUENCE 455 AA; 50392 MW; 1E20CBE609CB9C1E CRC64;
MLVTVTYEPE RDSGVDATVL GYLLHKHPGR VQTFSAPVGD IHVFYPEATA ERCTVAVMVE
VDPVALVRNK RFRGDAGALD HYVNDRPYVA SSMLAVAIGS VFRTAMTGRS DSYPELAASS
LPLRIDIPVV STRGREDDLV RRVFEPCGWT VTEQRIPLDP AFPDWEDSRF SSVTLTGEVR
LSDALRQLYV LLPVLDDAKH YWVGDDEIAK LERAGEGWLT RHPERDLITR RYLLHQRSLL
PQAAEAVDGP GETPLRVHRA EAVLAALHDV SARRVVDMGC GPGALLSRLA KDRSFTRIVG
VDVSARSLET AARVLRMDAA SDAERERIEL LHGSIVYRDD RLAGYDAMVL MEVVEHVDPS
RLGALEDAVF AGAHPASVVV TTPNAEYNAL YPDLAGGALR HDDHRFEWTR TQFRDWAEGV
AERHGYAVKY RPVGVEDAAL GAPTQLALFR REESA
//
