ID A0A0Q9F105_9GAMM Unreviewed; 2787 AA.
AC A0A0Q9F105;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=Non-ribosomal peptide synthetase {ECO:0008006|Google:ProtNLM};
GN ORFNames=ASE45_10945 {ECO:0000313|EMBL:KRD69624.1};
OS Lysobacter sp. Root96.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Lysobacter.
OX NCBI_TaxID=1736612 {ECO:0000313|EMBL:KRD69624.1, ECO:0000313|Proteomes:UP000050805};
RN [1] {ECO:0000313|EMBL:KRD69624.1, ECO:0000313|Proteomes:UP000050805}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Root96 {ECO:0000313|EMBL:KRD69624.1,
RC ECO:0000313|Proteomes:UP000050805};
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KRD69624.1, ECO:0000313|Proteomes:UP000050805}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Root96 {ECO:0000313|EMBL:KRD69624.1,
RC ECO:0000313|Proteomes:UP000050805};
RA Schulze-Lefert P.;
RT "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194}.
CC -!- PATHWAY: Siderophore biosynthesis. {ECO:0000256|ARBA:ARBA00004924}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP
CC synthases family. {ECO:0000256|ARBA:ARBA00008467}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the NRP synthetase
CC family. {ECO:0000256|ARBA:ARBA00029443}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRD69624.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LMJN01000002; KRD69624.1; -; Genomic_DNA.
DR RefSeq; WP_056305609.1; NZ_LMJN01000002.1.
DR STRING; 1736612.ASE45_10945; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000050805; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR CDD; cd17643; A_NRPS_Cytc1-like; 1.
DR CDD; cd12116; A_NRPS_Ta1_like; 1.
DR CDD; cd19531; LCL_NRPS-like; 1.
DR CDD; cd00833; PKS; 1.
DR Gene3D; 3.30.300.30; -; 2.
DR Gene3D; 3.30.70.3290; -; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR Gene3D; 3.40.50.980; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 1.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 2.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF00501; AMP-binding; 2.
DR Pfam; PF13193; AMP-binding_C; 2.
DR Pfam; PF00668; Condensation; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF00550; PP-binding; 3.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SMART; SM00823; PKS_PP; 3.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 2.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 2.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 1.
DR SUPFAM; SSF53901; Thiolase-like; 1.
DR PROSITE; PS00455; AMP_BINDING; 2.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 1.
DR PROSITE; PS52004; KS3_2; 1.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 3: Inferred from homology;
KW Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023160};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000050805};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 534..611
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 633..1059
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1544..1622
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2685..2762
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 1624..1649
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2787 AA; 299108 MW; 8D5BEDF865B13648 CRC64;
MNKLSDGSSQ YRPLACIPQL VEQQASTRPD AIAVTCEGAS LTYRELNSRA NQLAHRLRAL
GVGPETLVAI GLERSLEMVV GLLGILKSGG AYLPVDASYP RDRVQFMFED ARPAVLLTST
GQRGALPATD VAILLLDGDW AEFAAEPVHD PEPLAQLDNI AYVIYTSGST GKPKGCQVTH
ANVARLFVAT QPWFEFGPDD VWTFFHSHAF DFSVWEIWGA LIYGGRVVVV PFLVSRSPDA
FHALLVRERV TVLNQTPSAF RGLIHADLAS ASEPSALALR YVVFGGEALE LQMLRPWFER
HGDRYPRLIN MYGITETTVH VTYRPVARTD LERNAGSVIG EPIPDLRLYV LDPELVPVAA
GEVGEIHVAG AGVSRGYLNR PDLTAERFFD WQSPQGERVR LYKTGDLARS LADGDLEYLG
RSDHQVKIRG FRIETGEIES VLARHESVRA CAVIALDDRA GGEARLVAYV VPADVHASQA
SLRAHLATVL PDYMVPGAFV ELQALPLTEN GKLDRRALPA PARQRPELAH AFEPAIGALE
ASLCAAFGET LAVDNVGRHD NFFELGGDSL SAARLLQRLK QTGAHASAIP TAQLFRHPTP
ATLAGSLEGR GAAEIDPARY ASARRRESGG IAVEPIAIVA MAGRFPGAAD VEAFWRNLCE
GRDSITVFDP QDLDPAVSAQ DRDDPAYVPA RGVIDGVEQF DAAFFGIGPR EAELMDPQQR
IFLELAWECL ERAGHVPDAT SAPVGVFGGM FNASYFQRHV SAHPDLVDKV GAFQVMLNNE
KDFIATRVAH KLNLTGPAVS VHTACSTSLV AICQAVDSLT LGHCDMALAG GITVTCPPRS
GYFYQEGSML SPDGHTRPFD ASAQGTVFSD GAAIVLLKRL SDAVADGNQV FAVIRGGAVN
NDGGGKASFT APSSEGQAAV ISMAHDRAQV DPRSIGYVET HGTATPVGDP IEIEGLTRAF
RRGTEERGFC RIGSVKSNIG HLVIAAGATG VIKTALALAE QRIPATAHFK SPNPAIDFSG
SPFVVNDALT DWPVTDVPRR AGVSSFGVGG TNAHVVLEEA PKLPASDAAQ GPQLLMLSAR
TPTALSRAAT QLADRLDADP DINLADVAWT LAVGRKAFAH RIAVVADDVA GAAAQLRSPE
VAAAAARGKP VRQGDVVFLF PGQGATYPGM GRGLYDTEPA FRAAFDACAD ALLAELGFDL
RERVFSSDPE ALLPTAIMQP ATFAIEYSVA QLWISRGIVP AAMIGHSIGE FVAATLAGVF
ALPDALKLVA RRGALMQAQP AGGMLSVRLA WDELLARLPA DLSPAAENAP GSVVVSGTLE
AIARFQAELE ADGVACRALR TSHAFHSQMM EPVVAPFRAE VAALQLSAPL IPIVSTATGD
WLEEAAARSP DYWASHLRDP VRFATAVGKV LETPSRVLLE VGPRATLSGL SRQHPALLKQ
QATAVSSLAD DPATEASSFL SAAGQLWCRG TPIDIAAFDR RHSRKRVLLP TYPFERQRFW
VEAAASSNVV QLPSASAVHP VAIVPGPASA AEDATVPATS GAPDRHARLV SQLSDIFEDV
VGLDLSEVDD EANFMELGLD SLMLTQVALR LQKIFSVKLT FRQLMGECSS LQRLATMLDA
QLPPEPEAMP ASAAESAAPA GASESESGEN LARRLLEQQM QLLQQQLSEL DTSQGATAVA
MPGDEAAQPM HTGHDPFAGG APALLAPTTE PQREIWLADQ LGPEASLAFN LSVSLQFHGD
LNLEFMRDAL QELVDRHESL RASIGADGET FCVLERSALL LPIADLSSLN PIDRQMAVDE
RIRLTVETPF ALDQGPLFRA ELLRLAEDEH LLLLTAHHIV CDGWSWWTIV RELGALYGKR
FEMTAQPLPD AASFVGYALA EAGRQGDESQ AADEAYWLSR FATGTPVLEL PTDRPRPPNR
SFASARLDHV LDSGLLEALR RTGAARGVGM FATLLAGFSA LLSRLSSQSD VVVGIPAAGQ
AVCGADHLVG HCVNLLPLRF ELDSTKSFAH AIDDAQAMLL DALEHQRYTF GTLLRKLRLR
RDPARIPLVS VLFNLDQAMD QECTAFRGVS MEFDSNPRSF ETFELFVNAV QAHGQLRLEC
QYNRDLFDAA TIRRWLRAYE ALLRAAIERE TADFASLPLV DAVARTELSS LQPAAVAYDR
ECRMHEYFEA QCDRTPDRVA LRAGPTSLSY AELEARSNRV AHVLREHGVR RGALVGLALD
RGPDMLAALL GILKAGAGYV PLDPQFPTER LAYMAADAGL AMLLTTREHA AHFDLRGRPV
LLFDEHAPQL ATAAATRLGR DEYAAQPEDP AYVIYTSGST GRPKGVIVPH RAVSNFLDSM
RREPGLAAQD RLMAVTTLSF DIAVLELLLP LAVGAQIVLA DRETAGDGAA LRRLLESSGA
TVMQGTPSSW RLLIDAGWNG NDGFKALCGG EPMAVDLATS LLSRCGSLWN VYGPTETTVW
STCARIAADP DGQAPDIHIG RPIGNTSVWI LDAHGELCPF GVPGEICIGG EGVTKGYLER
PDLTAERFVA DRYTATAAGA PGHDGVALLY RTGDRGSWRA DGNLEHLGRL DHQVKVRGYR
IELGEIEANL ATREEIARAL VIVREDRAND QRLVAYVVAT PGATIEEAAL RAHLRELLPA
YMLPQHFVVL DEIPLLPNGK IDRNALLPPV AKEEAVSPPP SATGAAADPR VRYLSEIWSE
LLGLPAGPDD NFFELGGHSM LAVQMANRVE RDTGRRIKLI RLGAETLAQV AAGLPAATED
AVPAGGVGGR ISSGLRRLFG LNQGSSP
//
