ID A0A0R2AEE7_9LACO Unreviewed; 807 AA.
AC A0A0R2AEE7;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=Urocanate reductase {ECO:0000256|RuleBase:RU366062};
DE EC=1.3.99.33 {ECO:0000256|RuleBase:RU366062};
GN ORFNames=FC26_GL002286 {ECO:0000313|EMBL:KRM61068.1};
OS Paucilactobacillus vaccinostercus DSM 20634.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Paucilactobacillus.
OX NCBI_TaxID=1423813 {ECO:0000313|EMBL:KRM61068.1, ECO:0000313|Proteomes:UP000051733};
RN [1] {ECO:0000313|EMBL:KRM61068.1, ECO:0000313|Proteomes:UP000051733}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 20634 {ECO:0000313|EMBL:KRM61068.1,
RC ECO:0000313|Proteomes:UP000051733};
RX PubMed=26415554; DOI=10.1038/ncomms9322;
RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X.,
RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E.,
RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J.,
RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E.,
RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y.,
RA Zhang H., O'Toole P.W.;
RT "Expanding the biotechnology potential of lactobacilli through comparative
RT genomics of 213 strains and associated genera.";
RL Nat. Commun. 6:8322-8322(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=A + dihydrourocanate = AH2 + urocanate; Xref=Rhea:RHEA:36059,
CC ChEBI:CHEBI:13193, ChEBI:CHEBI:17499, ChEBI:CHEBI:27247,
CC ChEBI:CHEBI:72991; EC=1.3.99.33;
CC Evidence={ECO:0000256|RuleBase:RU366062};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|RuleBase:RU366062};
CC Note=Binds 1 FAD per subunit. {ECO:0000256|RuleBase:RU366062};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|RuleBase:RU366062};
CC Note=Binds 1 FMN covalently per subunit.
CC {ECO:0000256|RuleBase:RU366062};
CC -!- SIMILARITY: Belongs to the FAD-dependent oxidoreductase 2 family.
CC FRD/SDH subfamily. {ECO:0000256|RuleBase:RU366062}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRM61068.1}.
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DR EMBL; AYYY01000043; KRM61068.1; -; Genomic_DNA.
DR RefSeq; WP_057779521.1; NZ_AYYY01000043.1.
DR AlphaFoldDB; A0A0R2AEE7; -.
DR STRING; 1423813.FC26_GL002286; -.
DR PATRIC; fig|1423813.3.peg.2330; -.
DR OrthoDB; 9806724at2; -.
DR Proteomes; UP000051733; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.360; -; 1.
DR Gene3D; 3.90.1010.20; -; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR Gene3D; 3.90.700.10; Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain; 1.
DR InterPro; IPR003953; FAD-binding_2.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR010960; Flavocytochrome_c.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR InterPro; IPR007329; FMN-bd.
DR InterPro; IPR005025; FMN_Rdtase-like.
DR InterPro; IPR027477; Succ_DH/fumarate_Rdtase_cat_sf.
DR NCBIfam; TIGR01813; flavo_cyto_c; 1.
DR PANTHER; PTHR43400:SF7; FAD_BINDING_2 DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR43400; FUMARATE REDUCTASE; 1.
DR Pfam; PF00890; FAD_binding_2; 2.
DR Pfam; PF04205; FMN_bind; 1.
DR Pfam; PF03358; FMN_red; 1.
DR SMART; SM00900; FMN_bind; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR SUPFAM; SSF52218; Flavoproteins; 1.
DR SUPFAM; SSF56425; Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|RuleBase:RU366062};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|RuleBase:RU366062};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU366062};
KW Reference proteome {ECO:0000313|Proteomes:UP000051733}.
FT DOMAIN 200..274
FT /note="FMN-binding"
FT /evidence="ECO:0000259|SMART:SM00900"
SQ SEQUENCE 807 AA; 87704 MW; EE69A79572DB928A CRC64;
MKLIGIVGTN AEKSYNRQLL TFMQRHFADQ AEIEILEIKD VPMFNESADQ NLPIIQTFDQ
KIIDADGVII STPEHNHSIP SALKSTLEWL SNDVHSFEGK PVMIVGASYH DQGSSRAQLH
LRQILDAPGI DAIVMPGNEF LLGHAQQAFD EHGDLNNEGT VSFLASCFKK FLRFTNMVKM
LNVPEDLKVN PGTYDVTAMG HNGKLPMSVE FSEDKIENIE IDTSAETAGI ADVVFEKIPA
QILSGQTLNV DTVSGASVTS KGVIDGVAEA VKLAGGDPDV LKNRPRPSAK RQVPPVEYET
DVVVIGAGGA GLTAAASVLQ RGQRVIVAEK FPSVGGNTVR TGGPMNAADP TWQNEFSANP
GERTTLQGIL NTDEATIDPA YVADFRLLKT QITKYLETTD DDNEYLFDST LFHRIQTYLG
GKRVDLAGHE IHGQYDLVKI LTDHALESVK WLESIGVEFD QTEVSMPVGA KWRRGHKPLK
NQGYAYVSAL KTFVEQRDGQ ILTETPAKKL IIEDGEVRGI IAQGPDDQKV IIHASAVILA
SGGFGANTKM VQQYNTYWQQ IDDDIQTSNS PAITGDGIKL GTSAGADLVG MGFSQMMPVS
DPETGELFTG LQVPPANFVM VNQQGKRFVN EYEGRDVLSK AAIANGSLFY LIADDKIKET
AYNTSQEKID QQVEDGTLYR ANTLAELAQQ IHVDPAVLTT TIAHYNSYVD AGTDPEFGKN
VFDLKVEQAP FYATPRKPAI HHTMGGLRID TQAHVLNEQG TVIPHLFAAG EVAGGIHAGN
RLGGNSLTDI FTFGRVAGKT AVSENQK
//
