ID A0A0R2CJ23_9LACO Unreviewed; 568 AA.
AC A0A0R2CJ23;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Rqc2 homolog RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
DE Short=RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN Name=rqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN ORFNames=FD19_GL000375 {ECO:0000313|EMBL:KRM88086.1};
OS Lacticaseibacillus thailandensis DSM 22698 = JCM 13996.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lacticaseibacillus.
OX NCBI_TaxID=1423810 {ECO:0000313|EMBL:KRM88086.1, ECO:0000313|Proteomes:UP000051789};
RN [1] {ECO:0000313|EMBL:KRM88086.1, ECO:0000313|Proteomes:UP000051789}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 22698 {ECO:0000313|EMBL:KRM88086.1,
RC ECO:0000313|Proteomes:UP000051789};
RX PubMed=26415554; DOI=10.1038/ncomms9322;
RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X.,
RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E.,
RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J.,
RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E.,
RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y.,
RA Zhang H., O'Toole P.W.;
RT "Expanding the biotechnology potential of lactobacilli through comparative
RT genomics of 213 strains and associated genera.";
RL Nat. Commun. 6:8322-8322(2015).
CC -!- FUNCTION: Part of the ribosome quality control system (RQC). Recruits
CC Ala-charged tRNA and directs the elongation of stalled nascent chains
CC on 50S ribosomal subunits, leading to non-templated C-terminal Ala
CC extensions (Ala tail). The Ala tail promotes nascent chain degradation.
CC May add between 1 and at least 8 Ala residues. Binds to stalled 50S
CC ribosomal subunits. {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SUBUNIT: Associates with stalled 50S ribosomal subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SIMILARITY: Belongs to the NEMF family. {ECO:0000256|HAMAP-
CC Rule:MF_00844}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRM88086.1}.
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DR EMBL; AYZK01000001; KRM88086.1; -; Genomic_DNA.
DR RefSeq; WP_056968983.1; NZ_AYZK01000001.1.
DR AlphaFoldDB; A0A0R2CJ23; -.
DR STRING; 1423810.FD19_GL000375; -.
DR PATRIC; fig|1423810.4.peg.379; -.
DR Proteomes; UP000051789; Unassembled WGS sequence.
DR GO; GO:0043023; F:ribosomal large subunit binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0072344; P:rescue of stalled ribosome; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.970.40; fibrinogen binding protein from staphylococcus aureus domain like; 1.
DR Gene3D; 2.30.310.10; ibrinogen binding protein from staphylococcus aureus domain; 1.
DR HAMAP; MF_00844_B; RqcH_B; 1.
DR InterPro; IPR008532; NFACT_RNA-bd.
DR InterPro; IPR043682; RqcH_bacterial.
DR PANTHER; PTHR15239; NUCLEAR EXPORT MEDIATOR FACTOR NEMF; 1.
DR PANTHER; PTHR15239:SF6; RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF; 1.
DR Pfam; PF05670; NFACT-R_1; 1.
DR Pfam; PF18297; NFACT-R_2; 1.
DR Pfam; PF05833; NFACT_N; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|HAMAP-Rule:MF_00844};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00844}; Reference proteome {ECO:0000313|Proteomes:UP000051789};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW Rule:MF_00844};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|HAMAP-
KW Rule:MF_00844}.
FT DOMAIN 448..536
FT /note="NFACT RNA-binding"
FT /evidence="ECO:0000259|Pfam:PF05670"
FT COILED 292..319
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00844"
SQ SEQUENCE 568 AA; 64066 MW; 42A8A0258118CA27 CRC64;
MSFDGLFTHA MVRELQAALQ SGRVMKVSQP YPYELMLSIR ANRHNQTLLL SANPELARVQ
ITTIPFANPK VPTPFTMAMR KYLAAATLTA ITQVGADRII YFDFATRDEL GDSVSLRLVV
ELMGRHSNVI LVDQRTHKIL ELIRHVHPDE NRFRILLPGA EYVDPPYQDK LDPFMAQATD
YTSLVEQFGT DTAGLAKAIQ TRYQGFGRDA ALEMATRLQH GLPQAAWTGF MDAFDHPEPV
LIDAAKPQFA ATPLRTISGE QHSYPTLSAL LDAFYAQRAE RARVQEVGSK LIRVLRTNTE
RLSKKLKKLQ HTLDESDRAD ELRIRGEILT TYLRDVPRGA KSVELPNFYD DMRPIKIKLA
PELGPNQNAQ KYFTRYQKLR KAVSHVHEQM AEAESELHYL QGVQAMVEIA APQDLVDIQT
ELTNAGYIRQ QRKAKKQRQP KVAQPERFWA SDGTVILVGK NNLQNEQLTL HTAAKNDIWL
HAKDIPGSHV IIKDADPSDR TLQEAASLAA YFSKARQSAN VPVDYIPVKR IRKPNGSKPG
FVVYTGQRTL AVTPTVELVD KLRHQPTM
//
