ID A0A0R2D4G1_9LACO Unreviewed; 571 AA.
AC A0A0R2D4G1;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Rqc2 homolog RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
DE Short=RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN Name=rqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN ORFNames=FC24_GL000790 {ECO:0000313|EMBL:KRM98943.1};
OS Loigolactobacillus rennini DSM 20253.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Loigolactobacillus.
OX NCBI_TaxID=1423796 {ECO:0000313|EMBL:KRM98943.1, ECO:0000313|Proteomes:UP000051638};
RN [1] {ECO:0000313|EMBL:KRM98943.1, ECO:0000313|Proteomes:UP000051638}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 20253 {ECO:0000313|EMBL:KRM98943.1,
RC ECO:0000313|Proteomes:UP000051638};
RX PubMed=26415554; DOI=10.1038/ncomms9322;
RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X.,
RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E.,
RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J.,
RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E.,
RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y.,
RA Zhang H., O'Toole P.W.;
RT "Expanding the biotechnology potential of lactobacilli through comparative
RT genomics of 213 strains and associated genera.";
RL Nat. Commun. 6:8322-8322(2015).
CC -!- FUNCTION: Part of the ribosome quality control system (RQC). Recruits
CC Ala-charged tRNA and directs the elongation of stalled nascent chains
CC on 50S ribosomal subunits, leading to non-templated C-terminal Ala
CC extensions (Ala tail). The Ala tail promotes nascent chain degradation.
CC May add between 1 and at least 8 Ala residues. Binds to stalled 50S
CC ribosomal subunits. {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SUBUNIT: Associates with stalled 50S ribosomal subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SIMILARITY: Belongs to the NEMF family. {ECO:0000256|HAMAP-
CC Rule:MF_00844}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRM98943.1}.
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DR EMBL; AYYI01000022; KRM98943.1; -; Genomic_DNA.
DR RefSeq; WP_057873531.1; NZ_AYYI01000022.1.
DR AlphaFoldDB; A0A0R2D4G1; -.
DR STRING; 1423796.FC24_GL000790; -.
DR PATRIC; fig|1423796.3.peg.807; -.
DR OrthoDB; 9766163at2; -.
DR Proteomes; UP000051638; Unassembled WGS sequence.
DR GO; GO:0043023; F:ribosomal large subunit binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0072344; P:rescue of stalled ribosome; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.970.40; fibrinogen binding protein from staphylococcus aureus domain like; 1.
DR Gene3D; 2.30.310.10; ibrinogen binding protein from staphylococcus aureus domain; 1.
DR HAMAP; MF_00844_B; RqcH_B; 1.
DR InterPro; IPR008532; NFACT_RNA-bd.
DR InterPro; IPR043682; RqcH_bacterial.
DR PANTHER; PTHR15239; NUCLEAR EXPORT MEDIATOR FACTOR NEMF; 1.
DR PANTHER; PTHR15239:SF6; RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF; 1.
DR Pfam; PF05670; NFACT-R_1; 1.
DR Pfam; PF18297; NFACT-R_2; 1.
DR Pfam; PF05833; NFACT_N; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|HAMAP-Rule:MF_00844};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00844}; Reference proteome {ECO:0000313|Proteomes:UP000051638};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW Rule:MF_00844};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|HAMAP-
KW Rule:MF_00844}.
FT DOMAIN 450..538
FT /note="NFACT RNA-binding"
FT /evidence="ECO:0000259|Pfam:PF05670"
FT COILED 295..322
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00844"
SQ SEQUENCE 571 AA; 65156 MW; 1B7B625346CC7E4F CRC64;
MSFDGVFTHA MTNELAPLLT GGRLSRISQP YPNELILTLR SKRHNYPLLL SAHPSYARLQ
VTEIPFVNPE KPTNFTMTLR KYLDGAILKT VQQMQNDRVV HLTFTARNEI GDLEDLVLVI
EMMGRHSNII LVNQANHKII DTIRHVGHDQ NRYRLLLPGA EYIAPPKQSL ANPFQENKPF
YLSLPKTADI FAQAKQLQKT YQGFGFDTAV ELATRLNQAT DKQSTWQNFF ATIAQKPEPT
ITEDTNKKKR QFSPCKFVSE SGKQTTYPSL SLMLDDFYRN KAQLDRVRQQ GSELIRLVHN
EIKKNKKKYR KLEKTLANSE KADTYRIKGE LLTTYLNHVK RGMTTITLPN FYAAEKPTKI
SLSNQLSPSQ NAQKYFTKYQ KLKHAVHFVK QQLGETQAEI DYFQGILTQI EIAAPEDLQD
IQTELRQQGY LKSKQKQKKR RHKLSQPEAF YASDGTKILV GKNNLQNDRL TLKKARKTDI
WLHTKNIPGS HVVIMAADPS ETTKLEAANL AAYYSKARLS ASVPVDVVAI KKVHKPKGAK
PGFVIYTGQK TLSVTPDEKL VQKLRQPPMK K
//
