ID A0A0R2HLL7_9FIRM Unreviewed; 694 AA.
AC A0A0R2HLL7;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN ORFNames=IV49_GL000302 {ECO:0000313|EMBL:KRN50292.1};
OS Kandleria vitulina DSM 20405.
OC Bacteria; Bacillota; Erysipelotrichia; Erysipelotrichales;
OC Coprobacillaceae; Kandleria.
OX NCBI_TaxID=1410657 {ECO:0000313|EMBL:KRN50292.1, ECO:0000313|Proteomes:UP000051841};
RN [1] {ECO:0000313|EMBL:KRN50292.1, ECO:0000313|Proteomes:UP000051841}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 20405 {ECO:0000313|EMBL:KRN50292.1,
RC ECO:0000313|Proteomes:UP000051841};
RX PubMed=26415554; DOI=10.1038/ncomms9322;
RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X.,
RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E.,
RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J.,
RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E.,
RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y.,
RA Zhang H., O'Toole P.W.;
RT "Expanding the biotechnology potential of lactobacilli through comparative
RT genomics of 213 strains and associated genera.";
RL Nat. Commun. 6:8322-8322(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRN50292.1}.
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DR EMBL; JQBL01000011; KRN50292.1; -; Genomic_DNA.
DR RefSeq; WP_031589139.1; NZ_JQBL01000011.1.
DR AlphaFoldDB; A0A0R2HLL7; -.
DR PATRIC; fig|1410657.5.peg.316; -.
DR Proteomes; UP000051841; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 2.60.40.2320; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 2.
DR Pfam; PF02922; CBM_48; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000051841}.
FT DOMAIN 220..605
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 694 AA; 80785 MW; 3EE61BB1EE1757C0 CRC64;
MQQESARMYA YAISFDTIKV YLSKNYYNGI SSKFYLKDLE TSEHELLHGD TAGEEDGYNT
YLFHTSFKIG KSYRVSDAYG LTCFLDFSRL PLCKEFDDHF YYDGDDLGPA YTPYATSFKV
WAPTATSVIV KLKHYDDIKL YEMKRLDKGV FALRIFKDLE NYEYTYLIGN NDQVIESCDP
YAYASTSNQR SSIIVDLNKI RKKTYVLPPL EHKTDAVIYE LHVRNFSVDS YGNLRHKGKF
LAFAEEGNKT MMNEKSGVDY LEDLGVTHIQ LMPINDYATV DEDHPYELYN WGYDPAQYNV
TEGSYVTIPN DGYRRIQECR DMIDAIHKHG MRVILDVVYN HMHDIFDNAL AKTVPHYFFR
TDENGYLTNG TWCGNDMNST AKMCRKYILD MCKRWQTLYG VDGYRMDLMG ILDVETVNAI
EEQGRSIDPS FMLYGEGWNM GTLPIEEKAI IENNGKMPQV GFFNDFFRDT MRGNNQMEIK
GYTSGDTYKT NEAIFAMCDY NKFSSIEQSI NYVECHDNAT VYDKFVVCNR DEGEDYIRRR
CDLALVMTLL SQGVPFLHAG EEFYDTKKGN ANSYNAGDEV NQFDWGRRDQ NMKTVHLVKD
LIRLRKENKC FRYYDFEDMR NHISINNINH RMIEYILTQD EGSYHEFRIY VNPSLDNIGI
SIEDSFKILQ KADHEEIQNG YMNVAPCSFV IAAR
//
