ID A0A0S6VSD7_9BACT Unreviewed; 693 AA.
AC A0A0S6VSD7;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=Isoamylase {ECO:0000313|EMBL:GAK50360.1};
GN ORFNames=U14_01589 {ECO:0000313|EMBL:GAK50360.1};
OS Candidatus Moduliflexus flocculans.
OC Bacteria; Candidatus Moduliflexota; Candidatus Moduliflexia;
OC Candidatus Moduliflexales; Candidatus Moduliflexaceae.
OX NCBI_TaxID=1499966 {ECO:0000313|EMBL:GAK50360.1};
RN [1] {ECO:0000313|EMBL:GAK50360.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Sekiguchi Y., Ohashi A., Parks D.H., Yamauchi T., Tyson G.W.,
RA Hugenholtz P.;
RT "First genomic representation of candidate bacterial phylum KSB3 points to
RT enhanced environmental sensing as a trigger of wastewater bulking.";
RL PeerJ 3:e740-e740(2015).
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000256|ARBA:ARBA00004229}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
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DR EMBL; DF820456; GAK50360.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S6VSD7; -.
DR STRING; 1499966.U14_01589; -.
DR HOGENOM; CLU_011725_1_1_0; -.
DR Proteomes; UP000030700; Unassembled WGS sequence.
DR GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR GO; GO:0019156; F:isoamylase activity; IEA:UniProt.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11326; AmyAc_Glg_debranch; 1.
DR CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR011837; Glycogen_debranch_GlgX.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR048650; ISOA1-3-like_C.
DR NCBIfam; TIGR02100; glgX_debranch; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF21156; ISOA1-3_C; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Chloroplast {ECO:0000256|ARBA:ARBA00022528};
KW Plastid {ECO:0000256|ARBA:ARBA00022640};
KW Reference proteome {ECO:0000313|Proteomes:UP000030700};
KW Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT DOMAIN 169..575
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 693 AA; 77566 MW; BD7A90BBE366BA90 CRC64;
MPNKTTSEPR SKAPAVSQGN SFPLGASATP QGVNFSIYSK HATGIELLFF NNVADGKPAQ
TFGFDQITNR TGNYWHLFVP GVKAGQIYGY RVSGEFAPEQ GLRFDAEKIL LDPYGLGVAI
PSNYSRDAAK KPGDNTAVAM KSVVVDPKAY DWEGDAPLRN PSSRTIVYEM HVRGFTRHSN
SGVAEKRRGT YAGLIEKIPY LKALGITAVE LLPVFQFDPQ DSSPGHINYW GYAPISYFAP
HQAYSSRQDP LGPVDEFRDM VKALHRAGIE VILDVVYNHT AEGDETGPTL CFRGLENEAY
YILKPDKSGY ENYTGCGNTL NTNEPICRRI VLDSLRYWVQ EMHVDGFRFD LASILSRDAS
GQPLANPPVL WDIDSDPVLA GTKIIAEAWD AAGLYQVGHF IGDNWKEWNG RFRDDVRRFL
RGDEQTVPKL ADRLLGSPEI YGHEGREAEQ SVNFVTCHDG FTLYDLVSYN QKHNEANGEE
NRDGSNDNCS WNCGVEGPTD DPAIKALRLR QMKNFHAINM LSLGLPMMLM GDEVGHSQQG
NNNTYCQDNE LNWFNWELVK QNAELHRFVS LLAARRVQRD IQAELHRLNL IEVVRRSKIE
WHGVKLSQPD WGDNSHALAL SAHSPGLTFY WILNAYWEPL TFELPGQPGQ WKRWVDTSLN
SPDDIVEWEA APAVSGETYT AGARSVVVLF TQA
//
