ID A0A0S6VWD0_9BACT Unreviewed; 487 AA.
AC A0A0S6VWD0;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=Uncharacterized methyltransferase PH0819 {ECO:0000313|EMBL:GAK50516.1};
GN ORFNames=U14_01747 {ECO:0000313|EMBL:GAK50516.1};
OS Candidatus Moduliflexus flocculans.
OC Bacteria; Candidatus Moduliflexota; Candidatus Moduliflexia;
OC Candidatus Moduliflexales; Candidatus Moduliflexaceae.
OX NCBI_TaxID=1499966 {ECO:0000313|EMBL:GAK50516.1};
RN [1] {ECO:0000313|EMBL:GAK50516.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Sekiguchi Y., Ohashi A., Parks D.H., Yamauchi T., Tyson G.W.,
RA Hugenholtz P.;
RT "First genomic representation of candidate bacterial phylum KSB3 points to
RT enhanced environmental sensing as a trigger of wastewater bulking.";
RL PeerJ 3:e740-e740(2015).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; DF820456; GAK50516.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S6VWD0; -.
DR STRING; 1499966.U14_01747; -.
DR HOGENOM; CLU_021572_4_3_0; -.
DR Proteomes; UP000030700; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF12; BLL1977 PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS01278; MTTASE_RADICAL; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Methyltransferase {ECO:0000313|EMBL:GAK50516.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000030700};
KW Transferase {ECO:0000313|EMBL:GAK50516.1}.
FT DOMAIN 1..144
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 194..425
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 487 AA; 55676 MW; 356190A7AC30C3A6 CRC64;
MKVMLCLPPG GYFAERWSQG SFMPSLGVLY LAAVLEQQKI DVEVVPADVL RMTWDELGRK
FETDKPDIIG ITTTTENRFQ SFRLAQVAKR ARPQAFVVVG GPHFTGTARD TLSHLPYIDG
VVSGEGEVTI VELAQALQAR DSLRKVDGLS FYEDGQIIEN TPRQRIPDLN MLPLPARHLI
PYDKYNFKLD VPGKGLQPAA NIMTSRGCPF HCTFCATPNN WGRRVRGLSP ENVLKEVEYV
IEHYGAKVIW FYDDTFNYNP KRTTEICDMI IERKLDIKWY CELRVDLMTK ELTEKMAQAG
MFYAGFGIES GNHRVAQEIV KKVATLDQAR QFINWALECG VTPNPFFMFS HPTETWAEAQ
ETMAIIEEMK DRCDISVAIA HVYPGTELER RAREEGKIPA DFSWTNPNDH RVVLLPAAQG
HVPLYMDKLT WWQISELMFH FAGAKKKFSL LRKIPTVIQN IKSFADFQRY AIMFVAMLTF
KLKKMLK
//
