ID A0A0S8DEB4_9BACT Unreviewed; 929 AA.
AC A0A0S8DEB4;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE Flags: Fragment;
GN ORFNames=AMK70_07125 {ECO:0000313|EMBL:KPK34563.1};
OS Nitrospira bacterium SG8_35_1.
OC Bacteria; Nitrospirota; Nitrospiria.
OX NCBI_TaxID=1704024 {ECO:0000313|EMBL:KPK34563.1, ECO:0000313|Proteomes:UP000052010};
RN [1] {ECO:0000313|EMBL:KPK34563.1, ECO:0000313|Proteomes:UP000052010}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SG8_35_1 {ECO:0000313|EMBL:KPK34563.1};
RX PubMed=25922666; DOI=10.1186/s40168-015-0077-6;
RA Baker B.J., Lazar C.S., Teske A.P., Dick G.J.;
RT "Genomic resolution of linkages in carbon, nitrogen, and sulfur cycling
RT among widespread estuary sediment bacteria.";
RL Microbiome 3:14-14(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00560}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPK34563.1}.
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DR EMBL; LJTK01000100; KPK34563.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S8DEB4; -.
DR Proteomes; UP000052010; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF48; ATP-DEPENDENT HELICASE_NUCLEASE SUBUNIT A; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 1..306
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 318..600
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KPK34563.1"
SQ SEQUENCE 929 AA; 106782 MW; 030CC80D45D7D51B CRC64;
DPNYRVEEAT ESEMSWEETL YQVLMDAGRG LKGHDLLLDT LSEKGFRGLD YLRTTMDYLF
SKTPFSLEAD IAHHVPENFN TTLEELKSWP GLADSIDGYK DYFNADTAPV TEAIEKIFLT
EKKTPRKRPV AALKNIPAYH DWAAAMHHYW KEKKKGALNK QSRGIMDIFM ACIQKYSCKK
NNLGILDFSD LEYLAYKMLT GHPQWANILY AFDEKTDHIL VDEFQDTNSF QWEIINRLTE
EWRSGLGAKR DEGVQPTIFF VGDEKQSIYY FRGANVEIFS GARKNMVKWL GNEFLYDEAK
ENFRSRSSIV EFTNYVFSRI MQAGDRSFPW VTSYGAFKAQ REGDPGNVEL IILEQEVKNT
SAAKEYEADL LARKIDSIVN TMQIQGRSDT EGRPCRYEDI AILLRKRTHL KIFEEALRRE
DIPFVAVKGI GFYQEPEVAM LRALVYFLSN PGDDYSLYIL LKSPLFYFDN NMILSLIRSG
NGSLYSKLLM SSHEKDAQTE QTVTLLKQWL ADLPSMSIAS LIEQALFGTR AWTFLHDPQC
RANVKKFINI MENLEAQGKS IIKIRDYLER TCRRSDEPKA NVNTEGMDAV RIMTIHAAKG
LEFPVVFVPG LEEPFTHSRD TSLLYERSGH FYYKSIPEAS IRRDDQDFLL HMAKEEEEQK
RLFYVAVTRA EEALFLSGRG DGAQDSFMGF LESSLGLEKE GSLYTTQADI PGFSIATDSD
MAETVTQPRL KASATPDLQD IQVLPLSLRK TSPWKTVTES SDIRRGHGGD WPLLGDVLHR
IFEATSKGHL KKDAIHAQAA RMLQAKGLAH DDVLDKLDVI ERQVAFLKEN GIWQDIIVPR
EESFAELPFV IKTAEAVYSG RIDRIIKYNN IYNIYDYKTF PVEEKEMDHY LKGYFFQLTI
YRQAVQEIFQ TEDVKTYIVF THTGKIREL
//
