ID   A0A0S8IH77_9BACT        Unreviewed;       771 AA.
AC   A0A0S8IH77;
DT   17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT   17-FEB-2016, sequence version 1.
DT   27-MAR-2024, entry version 30.
DE   SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:KPK96880.1};
GN   ORFNames=AMJ95_11890 {ECO:0000313|EMBL:KPK96880.1};
OS   Omnitrophica WOR_2 bacterium SM23_72.
OC   Bacteria; Candidatus Omnitrophota.
OX   NCBI_TaxID=1703777 {ECO:0000313|EMBL:KPK96880.1, ECO:0000313|Proteomes:UP000051899};
RN   [1] {ECO:0000313|EMBL:KPK96880.1, ECO:0000313|Proteomes:UP000051899}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SM23_72 {ECO:0000313|EMBL:KPK96880.1};
RX   PubMed=25922666; DOI=10.1186/s40168-015-0077-6;
RA   Baker B.J., Lazar C.S., Teske A.P., Dick G.J.;
RT   "Genomic resolution of linkages in carbon, nitrogen, and sulfur cycling
RT   among widespread estuary sediment bacteria.";
RL   Microbiome 3:14-14(2015).
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KPK96880.1}.
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DR   EMBL; LJUU01000071; KPK96880.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0S8IH77; -.
DR   PATRIC; fig|1703777.3.peg.516; -.
DR   Proteomes; UP000051899; Unassembled WGS sequence.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01335; Radical_SAM; 1.
DR   CDD; cd02068; radical_SAM_B12_BD; 1.
DR   Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR   Gene3D; 3.20.20.370; Glycoside hydrolase/deacetylase; 1.
DR   Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR   InterPro; IPR006158; Cobalamin-bd.
DR   InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR   InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR   InterPro; IPR034466; Methyltransferase_Class_B.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR006879; YdjC-like.
DR   PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR   PANTHER; PTHR43409:SF17; MG-PROTOPORPHYRIN IX MONOMETHYL ESTER OXIDATIVE CYCLASE 66KD SUBUNIT; 1.
DR   Pfam; PF02310; B12-binding; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF04794; YdjC; 1.
DR   SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SMART; SM00729; Elp3; 1.
DR   SUPFAM; SSF88713; Glycoside hydrolase/deacetylase; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   PROSITE; PS51332; B12_BINDING; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   4: Predicted;
KW   4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW   Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW   Iron {ECO:0000256|ARBA:ARBA00022485};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT   DOMAIN          15..149
FT                   /note="B12-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51332"
FT   DOMAIN          199..434
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|PROSITE:PS51918"
SQ   SEQUENCE   771 AA;  88628 MW;  106554E28ECE6CBB CRC64;
     MAPKIIFINP PLTTQQRYGA LSSAGASEPP LGLAYLGAVT REMGIRTILL DACALRLSLE
     ESLKSVIKEQ PDFVGLTLST MSVGVASKLA EGIKNVLKRC TIIVGGPHLS SLAQQTLQDN
     PSFDVGVVGE GERTLEELLR ALECGRELSG IRGIVFRKEN RVFVNSPREQ IQDLDSLPFP
     AFDLLPQPNR YYRPTTQSIL RLPSVSLVTS RGCMGKCLFC DRKTFGNAIR LHSASYIVEM
     IEYLQKDFGI KGIMFEEDNF MLFHKRLVEF AELIRKRRIK IAWSAQSRVD TINDEKLRLA
     RSCGCWQILY GIESGSQKIL DFLKKQVTND QIEEAVHLTK KRGIYTKGFF MIGNPQESLK
     TLRETQEMIM RLPLDDVSLT FFTPYPGADI WPSILQHGRL EGGWEYYTCF HPVFIPFGLT
     KEILVKAQKG IWQSFYSRPR VIASYVRRLR SFSLLREFYR SWRLLSEYVR RSAIRKTLIV
     TAEDFGLNSG INQGIERLVM KGMIRGVSLV STGEAFFEAA KIAKNTPMLE VGVHLCLLET
     EPVLSRPEVS TLLGKSGRFE NKFIRFLLRY MGGRIRAEHI HRELVAQIEK VRQQGFEIIY
     LNGHQHIHML PGIFQMTIEL AKDYDIPFVR LPLVPLDSRF FFKKVPWRRK LGQLALNLLS
     SIYRKKLEKN GLFYSDYSYG FMESGLLSLE HLKDIFLSLR QGQSELFCHP AEENKSLQKR
     FGHWKYHWQE ESRALDSQEV KEMMDSLKIR LSTFRQKEQQ KQNQDMILMD V
//