ID A0A0T5PFT1_9RHOB Unreviewed; 751 AA.
AC A0A0T5PFT1;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE SubName: Full=Malic enzyme {ECO:0000313|EMBL:KRS19884.1, ECO:0000313|EMBL:QEW29381.1};
DE EC=1.1.1.40 {ECO:0000313|EMBL:KRS19884.1, ECO:0000313|EMBL:QEW29381.1};
GN Name=tme {ECO:0000313|EMBL:QEW29381.1};
GN ORFNames=RIdsm_05225 {ECO:0000313|EMBL:QEW29381.1}, XM52_00240
GN {ECO:0000313|EMBL:KRS19884.1};
OS Roseovarius indicus.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Roseovarius.
OX NCBI_TaxID=540747 {ECO:0000313|EMBL:KRS19884.1, ECO:0000313|Proteomes:UP000051401};
RN [1] {ECO:0000313|EMBL:KRS19884.1, ECO:0000313|Proteomes:UP000051401}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B108 {ECO:0000313|EMBL:KRS19884.1,
RC ECO:0000313|Proteomes:UP000051401};
RA Li G., Lai Q., Shao Z., Yan P.;
RT "The draft genome sequence of Roseovarius indicus B108T.";
RL Submitted (APR-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:QEW29381.1, ECO:0000313|Proteomes:UP000325785}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 26383 {ECO:0000313|EMBL:QEW29381.1,
RC ECO:0000313|Proteomes:UP000325785};
RA Vollmers J., Petersen J.;
RT "Genetic Globetrotter - A new plasmid hitch-hiking vast phylogenetic and
RT geographic distances.";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SIMILARITY: In the N-terminal section; belongs to the malic enzymes
CC family. {ECO:0000256|ARBA:ARBA00007686}.
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DR EMBL; LAXI01000001; KRS19884.1; -; Genomic_DNA.
DR EMBL; CP031598; QEW29381.1; -; Genomic_DNA.
DR RefSeq; WP_057813242.1; NZ_LAXI01000001.1.
DR AlphaFoldDB; A0A0T5PFT1; -.
DR STRING; 540747.SAMN04488031_102276; -.
DR KEGG; rid:RIdsm_05225; -.
DR PATRIC; fig|540747.5.peg.47; -.
DR OrthoDB; 9805787at2; -.
DR Proteomes; UP000051401; Unassembled WGS sequence.
DR Proteomes; UP000325785; Chromosome.
DR GO; GO:0016746; F:acyltransferase activity; IEA:InterPro.
DR GO; GO:0004473; F:malate dehydrogenase (decarboxylating) (NADP+) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0008948; F:oxaloacetate decarboxylase activity; IEA:UniProtKB-EC.
DR GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR CDD; cd05311; NAD_bind_2_malic_enz; 1.
DR Gene3D; 3.40.50.10950; -; 1.
DR Gene3D; 3.40.50.10750; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR015884; Malic_enzyme_CS.
DR InterPro; IPR012301; Malic_N_dom.
DR InterPro; IPR037062; Malic_N_dom_sf.
DR InterPro; IPR012302; Malic_NAD-bd.
DR InterPro; IPR045213; Malic_NAD-bd_bact_type.
DR InterPro; IPR012188; ME_PTA.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR042113; P_AcTrfase_dom1.
DR InterPro; IPR042112; P_AcTrfase_dom2.
DR InterPro; IPR002505; PTA_PTB.
DR PANTHER; PTHR43237; NADP-DEPENDENT MALIC ENZYME; 1.
DR PANTHER; PTHR43237:SF4; NADP-DEPENDENT MALIC ENZYME; 1.
DR Pfam; PF00390; malic; 1.
DR Pfam; PF03949; Malic_M; 1.
DR Pfam; PF01515; PTA_PTB; 1.
DR PIRSF; PIRSF036684; ME_PTA; 1.
DR SMART; SM01274; malic; 1.
DR SMART; SM00919; Malic_M; 1.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00331; MALIC_ENZYMES; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR036684-2};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|PIRSR:PIRSR036684-3};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:KRS19884.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000051401}.
FT DOMAIN 20..153
FT /note="Malic enzyme N-terminal"
FT /evidence="ECO:0000259|SMART:SM01274"
FT DOMAIN 165..401
FT /note="Malic enzyme NAD-binding"
FT /evidence="ECO:0000259|SMART:SM00919"
FT ACT_SITE 96
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-1"
FT BINDING 78..85
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 138
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 139
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 164
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 289
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
SQ SEQUENCE 751 AA; 81577 MW; 524AEC49916DC47A CRC64;
MPNPKFSREE ALAFHLEPTP GKFEVTATVP MTTQRDLSLA YSPGVAVPCE EIARNPELAY
DYTNKGNLVA VISNGTAVLG LGNLGALGSK PVMEGKAVLF KRFADVNSID IELDTEDPED
FIKAVRLMGP TFGGINLEDI KAPECFIIEQ TLKEQMDIPV FHDDQHGTAV ICAAGLINAL
HLSGKKIEDC RIVLNGAGAA GIACIELLKS MGARHENCIA CDTKGVIYQG RQDGMNQWKS
AHAVSTELRT LEEAMKDADV FLGVSVKGAV TQDMVKSMAP DPVIFAMANP DPEITPEDAH
EVRPDAIVAT GRSDYPNQVN NVLGFPYLFR GALDVHARAI NDEMKIACAH ALARLAREDV
PDEVAIAYGR KLSFGRDYII PTPFDPRLIH TIPPAVARAC MDTGAARRPI VDMDAYELAL
KSRLDPTASI MRSINARARN AQARMIFAEG DDPRVLRAAV QYTRTGFGHA VVVGRDEDVK
QKLEAAGLGD AFDEIEITNA AKTEHLEEYK SFLYGKLQRR GFDQQDIHRL AARDRHVFSA
LMLAHGHGDG LVTGATRKSA HVLDRINHVF DADAEHGAVG VTTLLHKGRI VMITDTLVHE
WPDEEDLANI AERAADVARG LGIEPRVAFV SFSTFGYPRS ERAEKMHRAP MVLEARGVDF
EFEGEMTVDV ALNKAAQDYY PFQRLTGPAN ILVVPARHSA SISTKLMQEM AGATVIGPIL
AGIDKSIQIC SSTSTASDIL NMAVLAACKV G
//
