ID A0A0T6UTY1_9PSED Unreviewed; 264 AA.
AC A0A0T6UTY1;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 24-JAN-2024, entry version 25.
DE RecName: Full=2-keto-4-pentenoate hydratase {ECO:0000256|HAMAP-Rule:MF_01655};
DE EC=4.2.1.80 {ECO:0000256|HAMAP-Rule:MF_01655};
DE AltName: Full=2-hydroxypentadienoic acid hydratase {ECO:0000256|HAMAP-Rule:MF_01655};
GN Name=mhpD {ECO:0000256|HAMAP-Rule:MF_01655};
GN ORFNames=AO726_08940 {ECO:0000313|EMBL:KRW61440.1};
OS Pseudomonas sp. TTU2014-080ASC.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1729724 {ECO:0000313|EMBL:KRW61440.1, ECO:0000313|Proteomes:UP000051864};
RN [1] {ECO:0000313|EMBL:KRW61440.1, ECO:0000313|Proteomes:UP000051864}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TTU2014-080ASC {ECO:0000313|EMBL:KRW61440.1,
RC ECO:0000313|Proteomes:UP000051864};
RA Webb H.E., Bugarel M., Den Bakker H.C., Nightingale K., Granier S.A.,
RA Loneragan G.H.;
RT "Carbapenem-non-susceptible Bacteria Recovered from the Faeces of Dairy
RT Cattle.";
RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the conversion of 2-hydroxypentadienoic acid
CC (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid.
CC {ECO:0000256|HAMAP-Rule:MF_01655}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-
CC dienoate + H2O; Xref=Rhea:RHEA:22580, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:67152, ChEBI:CHEBI:73143; EC=4.2.1.80;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01655};
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01655};
CC -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC {ECO:0000256|HAMAP-Rule:MF_01655}.
CC -!- SIMILARITY: Belongs to the hydratase/decarboxylase family. MhpD
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_01655}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRW61440.1}.
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DR EMBL; LKKK01000002; KRW61440.1; -; Genomic_DNA.
DR RefSeq; WP_058067716.1; NZ_LKKK01000002.1.
DR AlphaFoldDB; A0A0T6UTY1; -.
DR STRING; 1729724.AO726_08940; -.
DR OrthoDB; 9792137at2; -.
DR UniPathway; UPA00714; -.
DR Proteomes; UP000051864; Unassembled WGS sequence.
DR GO; GO:0008684; F:2-oxopent-4-enoate hydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.90.850.10; Fumarylacetoacetase-like, C-terminal domain; 1.
DR HAMAP; MF_01655; MhpD; 1.
DR InterPro; IPR011234; Fumarylacetoacetase-like_C.
DR InterPro; IPR036663; Fumarylacetoacetase_C_sf.
DR InterPro; IPR023793; Keto_pentenoate-hydratase.
DR PANTHER; PTHR30143:SF0; 2-KETO-4-PENTENOATE HYDRATASE; 1.
DR PANTHER; PTHR30143; ACID HYDRATASE; 1.
DR Pfam; PF01557; FAA_hydrolase; 1.
DR SUPFAM; SSF56529; FAH; 1.
PE 3: Inferred from homology;
KW Aromatic hydrocarbons catabolism {ECO:0000256|ARBA:ARBA00022797,
KW ECO:0000256|HAMAP-Rule:MF_01655}; Lyase {ECO:0000256|HAMAP-Rule:MF_01655};
KW Reference proteome {ECO:0000313|Proteomes:UP000051864}.
FT DOMAIN 62..258
FT /note="Fumarylacetoacetase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF01557"
SQ SEQUENCE 264 AA; 27892 MW; 6639F5691ECFC9E4 CRC64;
MSVSFDTLEQ LAAALRKAER SGEGIEPLRD QIGLDGAEAA YAIQRINVAH AEASGRRVVG
RKIGLTNKKV QAQLGVDQPD FGTLFADMCY GDNDTVPFGR VMQPKIEAEI ALVLEKDLPR
IDTTYLDVLD ATGWVLPSLE IVGSRIKDWN IKFVDTVADN ASSGCFVLGG PARRIADVDL
RKAVMSMTRN GEVVSSGSGA ECLGHPLNAA AWLASTMARL GTPLKAGDVI LTGALGPMVA
VAAGDHFEAV IEGIGQVGVQ FSAE
//
