ID A0A0T7RRI6_SALET Unreviewed; 581 AA.
AC A0A0T7RRI6;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 24-JAN-2024, entry version 56.
DE RecName: Full=DNA primase {ECO:0000256|HAMAP-Rule:MF_00974};
DE EC=2.7.7.101 {ECO:0000256|HAMAP-Rule:MF_00974};
GN Name=dnaG {ECO:0000256|HAMAP-Rule:MF_00974,
GN ECO:0000313|EMBL:SUE45948.1};
GN ORFNames=B7N00_06690 {ECO:0000313|EMBL:EDG5016647.1}, B7N01_17085
GN {ECO:0000313|EMBL:EDG4996137.1}, B7N35_07785
GN {ECO:0000313|EMBL:EDG5125932.1}, B7N72_18700
GN {ECO:0000313|EMBL:EDG5372129.1}, B7N78_00920
GN {ECO:0000313|EMBL:EDG5278356.1}, B7N80_13255
GN {ECO:0000313|EMBL:EDG5289347.1}, B7N84_04535
GN {ECO:0000313|EMBL:EDG5265616.1}, DRU31_00205
GN {ECO:0000313|EMBL:EBS2450774.1}, E0916_02775
GN {ECO:0000313|EMBL:ECF1444899.1}, EJV93_00710
GN {ECO:0000313|EMBL:ECA2719767.1}, ERS008198_02190
GN {ECO:0000313|EMBL:CNU20781.1}, ERS008202_04515
GN {ECO:0000313|EMBL:CNV17375.1}, ERS008207_01168
GN {ECO:0000313|EMBL:CNT85717.1}, EWC73_14750
GN {ECO:0000313|EMBL:ECE8537449.1}, G0B02_20550
GN {ECO:0000313|EMBL:HAC6381460.1}, G0D18_02145
GN {ECO:0000313|EMBL:HAC6679488.1}, G2206_03825
GN {ECO:0000313|EMBL:HAE0433910.1}, NCTC5754_00751
GN {ECO:0000313|EMBL:SUE45948.1};
OS Salmonella enterica subsp. enterica serovar Bovismorbificans.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=58097 {ECO:0000313|EMBL:SUE45948.1, ECO:0000313|Proteomes:UP000254190};
RN [1] {ECO:0000313|Proteomes:UP000039541, ECO:0000313|Proteomes:UP000041314}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3476 {ECO:0000313|EMBL:CNV17375.1,
RC ECO:0000313|Proteomes:UP000039541}, A1104
RC {ECO:0000313|EMBL:CNU20781.1, ECO:0000313|Proteomes:UP000041314}, and
RC D4891 {ECO:0000313|EMBL:CNT85717.1,
RC ECO:0000313|Proteomes:UP000042394};
RG Pathogen Informatics;
RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:HAC6381460.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=BCW_3301 {ECO:0000313|EMBL:HAC6381460.1}, M123
RC {ECO:0000313|EMBL:HAC6679488.1}, and MS140073
RC {ECO:0000313|EMBL:HAE0433910.1};
RX PubMed=30286803;
RA Souvorov A., Agarwala R., Lipman D.J.;
RT "SKESA: strategic k-mer extension for scrupulous assemblies.";
RL Genome Biol. 19:153-153(2018).
RN [3] {ECO:0000313|EMBL:SUE45948.1, ECO:0000313|Proteomes:UP000254190}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC5754 {ECO:0000313|EMBL:SUE45948.1,
RC ECO:0000313|Proteomes:UP000254190};
RG Pathogen Informatics;
RA Doyle S.;
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:HAE0433910.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=BCW_3301 {ECO:0000313|EMBL:HAC6381460.1}, M123
RC {ECO:0000313|EMBL:HAC6679488.1}, and MS140073
RC {ECO:0000313|EMBL:HAE0433910.1};
RG NCBI Pathogen Detection Project;
RL Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:ECE8537449.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=136768 {ECO:0000313|EMBL:EDG4996137.1}, 138330
RC {ECO:0000313|EMBL:EDG5289347.1}, 143652
RC {ECO:0000313|EMBL:EDG5372129.1}, 273631
RC {ECO:0000313|EMBL:EDG5016647.1}, 330535
RC {ECO:0000313|EMBL:EDG5265616.1}, 333944
RC {ECO:0000313|EMBL:EDG5278356.1}, 337042
RC {ECO:0000313|EMBL:EDG5125932.1}, 387147
RC {ECO:0000313|EMBL:EBS2450774.1}, 470200
RC {ECO:0000313|EMBL:ECE8537449.1}, 598023
RC {ECO:0000313|EMBL:ECA2719767.1}, and 692616
RC {ECO:0000313|EMBL:ECF1444899.1};
RA Ashton P.M., Dallman T., Nair S., De Pinna E., Peters T., Grant K.;
RL Submitted (FEB-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA
CC molecules used as primers for DNA polymerase during DNA replication.
CC {ECO:0000256|HAMAP-Rule:MF_00974}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.;
CC EC=2.7.7.101; Evidence={ECO:0000256|HAMAP-Rule:MF_00974};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00974};
CC Note=Binds 1 zinc ion per monomer. {ECO:0000256|HAMAP-Rule:MF_00974};
CC -!- SUBUNIT: Monomer. Interacts with DnaB. {ECO:0000256|HAMAP-
CC Rule:MF_00974}.
CC -!- DOMAIN: Contains an N-terminal zinc-binding domain, a central core
CC domain that contains the primase activity, and a C-terminal DnaB-
CC binding domain. {ECO:0000256|HAMAP-Rule:MF_00974}.
CC -!- SIMILARITY: Belongs to the DnaG primase family. {ECO:0000256|HAMAP-
CC Rule:MF_00974}.
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DR EMBL; CQPD01000009; CNT85717.1; -; Genomic_DNA.
DR EMBL; CQPA01000014; CNU20781.1; -; Genomic_DNA.
DR EMBL; CQPC01000096; CNV17375.1; -; Genomic_DNA.
DR EMBL; AAGUWJ010000001; EBS2450774.1; -; Genomic_DNA.
DR EMBL; AAHTVM010000001; ECA2719767.1; -; Genomic_DNA.
DR EMBL; AAIJHK010000013; ECE8537449.1; -; Genomic_DNA.
DR EMBL; AAIKGE010000002; ECF1444899.1; -; Genomic_DNA.
DR EMBL; AAMEJW010000019; EDG4996137.1; -; Genomic_DNA.
DR EMBL; AAMEJY010000003; EDG5016647.1; -; Genomic_DNA.
DR EMBL; AAMEKY010000005; EDG5125932.1; -; Genomic_DNA.
DR EMBL; AAMEMC010000002; EDG5265616.1; -; Genomic_DNA.
DR EMBL; AAMEMH010000001; EDG5278356.1; -; Genomic_DNA.
DR EMBL; AAMEML010000013; EDG5289347.1; -; Genomic_DNA.
DR EMBL; AAMEMP010000026; EDG5372129.1; -; Genomic_DNA.
DR EMBL; DAAMEZ010000018; HAC6381460.1; -; Genomic_DNA.
DR EMBL; DAAMHM010000001; HAC6679488.1; -; Genomic_DNA.
DR EMBL; DAAQQK010000003; HAE0433910.1; -; Genomic_DNA.
DR EMBL; UGVQ01000002; SUE45948.1; -; Genomic_DNA.
DR RefSeq; WP_000918865.1; NZ_WFIN01000002.1.
DR AlphaFoldDB; A0A0T7RRI6; -.
DR SMR; A0A0T7RRI6; -.
DR Proteomes; UP000039541; Unassembled WGS sequence.
DR Proteomes; UP000041314; Unassembled WGS sequence.
DR Proteomes; UP000042394; Unassembled WGS sequence.
DR Proteomes; UP000254190; Unassembled WGS sequence.
DR Proteomes; UP000839929; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR CDD; cd03364; TOPRIM_DnaG_primases; 1.
DR Gene3D; 3.40.1360.10; -; 1.
DR Gene3D; 3.90.980.10; DNA primase, catalytic core, N-terminal domain; 1.
DR Gene3D; 1.20.50.20; DnaG, RNA polymerase domain, helical bundle; 1.
DR Gene3D; 3.90.580.10; Zinc finger, CHC2-type domain; 1.
DR HAMAP; MF_00974; DNA_primase_DnaG; 1.
DR InterPro; IPR016136; DNA_helicase_N/primase_C.
DR InterPro; IPR037068; DNA_primase_core_N_sf.
DR InterPro; IPR019475; DNA_primase_DnaB-bd.
DR InterPro; IPR006295; DNA_primase_DnaG.
DR InterPro; IPR013173; DNA_primase_DnaG_DnaB-bd_dom.
DR InterPro; IPR036977; DNA_primase_Znf_CHC2.
DR InterPro; IPR030846; DnaG_bac.
DR InterPro; IPR013264; DNAG_N.
DR InterPro; IPR034151; TOPRIM_DnaG_bac.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR002694; Znf_CHC2.
DR NCBIfam; TIGR01391; dnaG; 1.
DR PANTHER; PTHR30313; DNA PRIMASE; 1.
DR PANTHER; PTHR30313:SF2; DNA PRIMASE; 1.
DR Pfam; PF10410; DnaB_bind; 1.
DR Pfam; PF08278; DnaG_DnaB_bind; 1.
DR Pfam; PF08275; DNAG_N; 1.
DR Pfam; PF13155; Toprim_2; 1.
DR Pfam; PF01807; zf-CHC2; 1.
DR SMART; SM00766; DnaG_DnaB_bind; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SMART; SM00400; ZnF_CHCC; 1.
DR SUPFAM; SSF56731; DNA primase core; 1.
DR SUPFAM; SSF117023; DNA primase DnaG, C-terminal domain; 1.
DR SUPFAM; SSF57783; Zinc beta-ribbon; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW Rule:MF_00974};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00974};
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|HAMAP-Rule:MF_00974};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_00974};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_00974};
KW Primosome {ECO:0000256|ARBA:ARBA00022515, ECO:0000256|HAMAP-Rule:MF_00974};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00974};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00974};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|HAMAP-Rule:MF_00974};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|HAMAP-
KW Rule:MF_00974}.
FT DOMAIN 259..341
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT ZN_FING 40..64
FT /note="CHC2-type"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00974"
SQ SEQUENCE 581 AA; 65363 MW; 011B6E10AA55632F CRC64;
MAGRIPRVFI NDLLARTDIV DLIDVRVKLK KQGKNYHACC PFHNEKTPSF TVNGEKQFYH
CFGCGAHGNA IDFLMNYDKL EFVETVEELA AMHNLEIPYE AGTGLSQIER HQRQNLYQLM
NGLNDFYQQS LTHPAAKPAR DYLQKRGLSA EIIQRFAIGF APPGWDNALK RFGNNSDNKA
LLLDAGMLVN NEQGSTYDRF RNRVMFPIRD KRGRVIGFGG RVLGNDTPKY LNSPETDIFH
KGRQLYGLYE AQQYSAEPQR LLVVEGYMDV VALAQYDINY AVASLGTSTT ADHMHMLFRA
TNNVICCYDG DRAGRDAAWR ALETAMPYMT DGRQVRFMFL PDGEDPDTLV RKEGKAAFEA
RMEQAQPLST FLFNSLLPQV DLSSPDGSTQ LAALALPLIN QVPGDAHRIQ LRQTLGLKLG
IFDDSQLDRL VPKQAESGVS RPAPQLKRTT MRILIGLLVQ NPDLAPLVPP LDALDQNKLP
GLGLFKELVK TCLAQPGLTT GQLLELYRGT NDAATLEKLS MWDDIADKAI AEKTFTDSLN
HMFDSLLQLR QEELIARDRT HGLSSEERRE LWTLNQELAR K
//
