ID A0A0U4FGL4_9BACI Unreviewed; 515 AA.
AC A0A0U4FGL4;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=AOX59_03720 {ECO:0000313|EMBL:ALX47790.1};
OS Lentibacillus amyloliquefaciens.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Lentibacillus.
OX NCBI_TaxID=1472767 {ECO:0000313|EMBL:ALX47790.1, ECO:0000313|Proteomes:UP000050331};
RN [1] {ECO:0000313|EMBL:ALX47790.1, ECO:0000313|Proteomes:UP000050331}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LAM0015 {ECO:0000313|EMBL:ALX47790.1,
RC ECO:0000313|Proteomes:UP000050331};
RA Wang J.-L., He M.-X.;
RT "Complete genome sequence of strain Lentibacillus amyloliquefaciens
RT LAM0015T isolated from saline sediment.";
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CP013862; ALX47790.1; -; Genomic_DNA.
DR RefSeq; WP_068442020.1; NZ_CP013862.1.
DR AlphaFoldDB; A0A0U4FGL4; -.
DR STRING; 1472767.AOX59_03720; -.
DR REBASE; 137069; M.Lam15ORF3720P.
DR KEGG; lao:AOX59_03720; -.
DR OrthoDB; 9814572at2; -.
DR Proteomes; UP000050331; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Endonuclease {ECO:0000313|EMBL:ALX47790.1};
KW Hydrolase {ECO:0000313|EMBL:ALX47790.1};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Nuclease {ECO:0000313|EMBL:ALX47790.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000050331};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 14..163
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 178..480
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 484..511
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 515 AA; 59145 MW; B7D1FC5DC3283B43 CRC64;
MTTTEKQRQQ QADLQKKLWD IANDLRGNMD ASEFRNYILG LIFYRFLSEN TEQQVEQLLA
EDNITYEEAW QNDEYQDVLK QELIARIGYV IEPQDLFSHL VKKIENQTFE IEDLHRAINH
IEASTRGEDS EDEFERLFED MDLNSSRLGN TNAERTKLIS KVMMNLAALP FVQGDMEIDM
LGDAYEYLIG QFAATAGKKA GEFYTPQQVS KILAKIVTTG KKDLKNVYDP TCGSGSLLLR
VGREANVRHY YGQEYNNTTF NLARMNMLLH DVNYHNFSLA NGDTLEHPAV VDGQFEAVVA
NPPYSAKWSA DPSFLDDERF SNYGKLAPKS KADFAFVQHM IYHLDDNGKM AVVLPHGVLF
RGAAEETIRK YLIEEKNYLD AVIGLPSNLF FGTGIPTCIL VFKKCREQDE DVLFIDASQS
FEKGRNQNHL ADEDVNKIVE TFRNRETLDK FSYVSTLDEI RKNDYNLNIP RYVDTFEEEE
PVDLDKVQQD IQDIDQKIVG LEEEINGYLK ELGVL
//
