ID A0A0X3S0M0_9ACTN Unreviewed; 469 AA.
AC A0A0X3S0M0;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=L-lysine N6-monooxygenase MbtG {ECO:0000256|ARBA:ARBA00016406};
DE EC=1.14.13.59 {ECO:0000256|ARBA:ARBA00013076};
DE AltName: Full=Lysine 6-N-hydroxylase {ECO:0000256|ARBA:ARBA00032738};
DE AltName: Full=Lysine N6-hydroxylase {ECO:0000256|ARBA:ARBA00032493};
DE AltName: Full=Lysine-N-oxygenase {ECO:0000256|ARBA:ARBA00029939};
DE AltName: Full=Mycobactin synthase protein G {ECO:0000256|ARBA:ARBA00031158};
GN ORFNames=ADL25_41735 {ECO:0000313|EMBL:KUJ34295.1};
OS Streptomyces sp. NRRL F-5122.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1609098 {ECO:0000313|EMBL:KUJ34295.1, ECO:0000313|Proteomes:UP000054048};
RN [1] {ECO:0000313|Proteomes:UP000054048}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL F-5122 {ECO:0000313|Proteomes:UP000054048};
RA Ju K.-S., Doroghazi J.R., Metcalf W.W.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-lysine + NADPH + O2 = H2O + N(6)-hydroxy-L-lysine + NADP(+);
CC Xref=Rhea:RHEA:23228, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32551, ChEBI:CHEBI:57783, ChEBI:CHEBI:57820,
CC ChEBI:CHEBI:58349; EC=1.14.13.59;
CC Evidence={ECO:0000256|ARBA:ARBA00000168};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- PATHWAY: Siderophore biosynthesis. {ECO:0000256|ARBA:ARBA00004924}.
CC -!- SIMILARITY: Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-
CC oxygenase family. {ECO:0000256|ARBA:ARBA00007588}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUJ34295.1}.
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DR EMBL; LMWH01000290; KUJ34295.1; -; Genomic_DNA.
DR RefSeq; WP_059133162.1; NZ_LMWH01000290.1.
DR AlphaFoldDB; A0A0X3S0M0; -.
DR OrthoDB; 7527071at2; -.
DR Proteomes; UP000054048; Unassembled WGS sequence.
DR GO; GO:0047091; F:L-lysine 6-monooxygenase (NADPH) activity; IEA:UniProtKB-EC.
DR GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR025700; Lys/Orn_oxygenase.
DR PANTHER; PTHR42802:SF1; L-ORNITHINE N(5)-MONOOXYGENASE; 1.
DR PANTHER; PTHR42802; MONOOXYGENASE; 1.
DR Pfam; PF13434; Lys_Orn_oxgnase; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 2.
PE 3: Inferred from homology;
KW Monooxygenase {ECO:0000313|EMBL:KUJ34295.1};
KW Oxidoreductase {ECO:0000313|EMBL:KUJ34295.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000054048}.
SQ SEQUENCE 469 AA; 52199 MW; 3AA01357121DDF4D CRC64;
MTTPPHHEPP APRDLVGIGI GPFNLSLAAL AHPLAELDTA FYEQRPGFDW HPGLLIDGAT
LQVPFLADLV TLADPASPWS FLNYLKSCDR LFPFYFAERF HIQRAEYDAY CRWVSGNLPE
LHFGHQVDAV RWNGADKVFE VDYTRLGKDG AGEALGRTRT RNIVLGVGTE PYVPEPLRPL
VEAPGTPVIH AAEYLRHRTA FLAADHITVV GAGQSGAEVF LDLLRHRPVG RERIHWLART
EAFAPMEYSK LGLEHFTPDY TRYFHALAEP VRDGLVSAQW QLHKGIDADT IAAIHDELYR
RTLQGGWPDA VLTPGVHVRT AGRAAPSRIE LHLEHVQQNT RSRLATDAVV LATGYRERPL
GRILAGLEHR LSRDGSGRPR IDDRFRLVLD RAVTGSVYVQ NAELHTHGVG TPDLGLAAWR
SATILNSLTG REPYPLPRRT AFTTFGLRQP GLIPPTRRPM DLTPLGDGR
//
