ID   A0A0X3V5A8_9ACTN        Unreviewed;       478 AA.
AC   A0A0X3V5A8;
DT   13-APR-2016, integrated into UniProtKB/TrEMBL.
DT   13-APR-2016, sequence version 1.
DT   24-JAN-2024, entry version 34.
DE   RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE            EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
DE   Flags: Fragment;
GN   ORFNames=ADL12_15020 {ECO:0000313|EMBL:KUL39617.1};
OS   Streptomyces regalis.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=68262 {ECO:0000313|EMBL:KUL39617.1, ECO:0000313|Proteomes:UP000053923};
RN   [1] {ECO:0000313|Proteomes:UP000053923}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NRRL 3151 {ECO:0000313|Proteomes:UP000053923};
RA   Ju K.-S., Doroghazi J.R., Metcalf W.W.;
RL   Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Poorly processive, error-prone DNA polymerase involved in
CC       untargeted mutagenesis. Copies undamaged DNA at stalled replication
CC       forks, which arise in vivo from mismatched or misaligned primer ends.
CC       These misaligned primers can be extended by PolIV. Exhibits no 3'-5'
CC       exonuclease (proofreading) activity. May be involved in translesional
CC       synthesis, in conjunction with the beta clamp from PolIII.
CC       {ECO:0000256|ARBA:ARBA00025589}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC         Evidence={ECO:0000256|ARBA:ARBA00024632};
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
CC       {ECO:0000256|ARBA:ARBA00010945}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KUL39617.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; LLZG01000094; KUL39617.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0X3V5A8; -.
DR   Proteomes; UP000053923; Unassembled WGS sequence.
DR   GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR   GO; GO:0009432; P:SOS response; IEA:UniProt.
DR   CDD; cd03586; PolY_Pol_IV_kappa; 1.
DR   Gene3D; 3.30.70.270; -; 1.
DR   Gene3D; 3.40.1170.60; -; 1.
DR   Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR   Gene3D; 3.30.1490.100; DNA polymerase, Y-family, little finger domain; 1.
DR   HAMAP; MF_01113; DNApol_IV; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR036775; DNA_pol_Y-fam_lit_finger_sf.
DR   InterPro; IPR017961; DNA_pol_Y-fam_little_finger.
DR   InterPro; IPR022880; DNApol_IV.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR001126; UmuC.
DR   PANTHER; PTHR11076:SF33; DNA POLYMERASE KAPPA; 1.
DR   PANTHER; PTHR11076; DNA REPAIR POLYMERASE UMUC / TRANSFERASE FAMILY MEMBER; 1.
DR   Pfam; PF14520; HHH_5; 1.
DR   Pfam; PF00817; IMS; 1.
DR   Pfam; PF11799; IMS_C; 1.
DR   SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR   SUPFAM; SSF100879; Lesion bypass DNA polymerase (Y-family), little finger domain; 1.
DR   PROSITE; PS50173; UMUC; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000053923}.
FT   DOMAIN          7..189
FT                   /note="UmuC"
FT                   /evidence="ECO:0000259|PROSITE:PS50173"
FT   REGION          360..381
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          436..478
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_TER         478
FT                   /evidence="ECO:0000313|EMBL:KUL39617.1"
SQ   SEQUENCE   478 AA;  50634 MW;  F2D883DCDC4496D7 CRC64;
     MRNAPTILHL DMDAFYASVE QASKPSLRGK AVIVGGLGPR GVVATASYEA RVFGVHSAMP
     MAQARRLAPN AAYLVPRFGI YRDISEQVMA LLRELSPLVE PLSLDEAFVD LEAGGAAWDE
     GSARLVGTKL RADIRAATGL AGSVGLAASK MLAKIASEQA KPDGLVVIEP GTERAMLGPM
     SVRTLPGVGP ATGDHLRRAG IHTVEEIAEA GEDELVRLLG KAHGHALYAM ALAHDERPVV
     AEREAKSVSV EDTYDVDIHD RVRVGLEVQR LADRCVRRLR GAGLSGRTIV LKVRRYDFST
     LTRSETLRGP TDDPAVVREA AARLLDSVDT TGGVRLLGVG VSGLADYTQE DLFAQTAGEQ
     VEHPEAEGGQ EEPAEEPQAA PVERRWSAGH DVRHAEYGHG WVQGSGLGRV TVRFETPESS
     EPGRVRTFRV DDPGLEAADP LPLVRTRPGV AGGIGEPRGS GTAGALGTGD AGLPEARG
//