ID A0A0X3VAR4_9ACTN Unreviewed; 472 AA.
AC A0A0X3VAR4;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=L-lysine N6-monooxygenase MbtG {ECO:0000256|ARBA:ARBA00016406};
DE EC=1.14.13.59 {ECO:0000256|ARBA:ARBA00013076};
DE AltName: Full=Lysine 6-N-hydroxylase {ECO:0000256|ARBA:ARBA00032738};
DE AltName: Full=Lysine N6-hydroxylase {ECO:0000256|ARBA:ARBA00032493};
DE AltName: Full=Lysine-N-oxygenase {ECO:0000256|ARBA:ARBA00029939};
DE AltName: Full=Mycobactin synthase protein G {ECO:0000256|ARBA:ARBA00031158};
DE Flags: Fragment;
GN ORFNames=ADL12_11070 {ECO:0000313|EMBL:KUL41704.1};
OS Streptomyces regalis.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=68262 {ECO:0000313|EMBL:KUL41704.1, ECO:0000313|Proteomes:UP000053923};
RN [1] {ECO:0000313|Proteomes:UP000053923}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL 3151 {ECO:0000313|Proteomes:UP000053923};
RA Ju K.-S., Doroghazi J.R., Metcalf W.W.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-lysine + NADPH + O2 = H2O + N(6)-hydroxy-L-lysine + NADP(+);
CC Xref=Rhea:RHEA:23228, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32551, ChEBI:CHEBI:57783, ChEBI:CHEBI:57820,
CC ChEBI:CHEBI:58349; EC=1.14.13.59;
CC Evidence={ECO:0000256|ARBA:ARBA00000168};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- PATHWAY: Siderophore biosynthesis. {ECO:0000256|ARBA:ARBA00004924}.
CC -!- SIMILARITY: Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-
CC oxygenase family. {ECO:0000256|ARBA:ARBA00007588}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUL41704.1}.
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DR EMBL; LLZG01000060; KUL41704.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0X3VAR4; -.
DR OrthoDB; 7527071at2; -.
DR Proteomes; UP000053923; Unassembled WGS sequence.
DR GO; GO:0047091; F:L-lysine 6-monooxygenase (NADPH) activity; IEA:UniProtKB-EC.
DR GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR025700; Lys/Orn_oxygenase.
DR PANTHER; PTHR42802:SF1; L-ORNITHINE N(5)-MONOOXYGENASE; 1.
DR PANTHER; PTHR42802; MONOOXYGENASE; 1.
DR Pfam; PF13434; Lys_Orn_oxgnase; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 2.
PE 3: Inferred from homology;
KW Monooxygenase {ECO:0000313|EMBL:KUL41704.1};
KW Oxidoreductase {ECO:0000313|EMBL:KUL41704.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000053923}.
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KUL41704.1"
SQ SEQUENCE 472 AA; 52468 MW; 33A1140D6AA0225B CRC64;
PPGHEPDTPR DLVGIGIGPF NLSLAALAHP LTELDAVFYE QRSAFAWHPG LLIDGARLQV
PFLADLVTLA DPASPWSFLS YLRTRDRLFP FYFAERFHIQ RAEYDAYCRW VCENLPGLGF
GHQVDAVRFN PEHDVFEVDF TQLGPDGEAE ALGRTYTKNV VLGIGTEPDV PDPLKPLVEA
PGVPVIHAAD YLDHRETLLA ADHVTVIGSG QSGAEVFLDL LRRRPVGREG LHWLGRTDAF
APMEYSKLGL EHFTPDYTRY FHALAEPVRD KLVASQWQLH KGIDAGTLSA IHDELYRRTL
NGGWPDTVLT PGVRVRTAGR LATTKVELHL EHIHQNTRSR LTTDAVVLAT GYRERPLSRI
LAGLDPYLRL DSRERPRIDD QYRLVMDPSV TGSGCNIYVQ NAERHTHGIG TPDLGLAAWR
SAAILNSVTG REAYPLPTRT AFTTFGLDPA LPRQVRSRTI GAQRGTVVQL KG
//
